LOCUS       sequence-417848-       11528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal tagged NRF3 for miniTurbo proximity experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   LaPak KM, Saeidi S, Bok I, Wamsley NT, Plutzer IB, Bhatt DP, Luo J, 
            Ashrafi G, Ben Major M
  TITLE     Proximity proteomic analysis of the NRF family reveals the 
            Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor
            of NRF2.
  JOURNAL   Sci Signal. 2023 Dec 12;16(815):eadi9018. doi: 
            10.1126/scisignal.adi9018. Epub 2023 Dec 12.
  PUBMED    38085818
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-12-12"; volume: "16(815):eadi9018. doi"; 
            pages: " 10.1126/scisignal.adi9018. Epub 2023 Dec 12"
COMMENT     Sequence Label: p667-UBC-NRF3-V5-miniTurbo
FEATURES             Location/Qualifiers
     source          1..11528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7507..7531)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7533..7574
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7581..8351
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8357..8377)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8406..8994
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8459..8479)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8877..8888)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9008..9507
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9043..9066)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9440..9459
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9528..10127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9528..9547)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10024..10044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10265..10498
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10570..10691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10607..10626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10661..10680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10731..10866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10886..10905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10887..10905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10915..10932)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10915..10931)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10924..10946)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11073..11501
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11160..11179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11370..11391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 caactttgta caaaaaagtt ggcatgaagc acctgaagcg gtggtggtcg gccggcggcg
     5461 gcctcctgca cctcaccctc ctgctgagct tggcggggct ccgcgtagac ctagatcttt
     5521 acctgctgct gccgccaccc accctgctgc aggacgagct gctgttcctg ggcggcccgg
     5581 ccagctccgc ctacgcgctc agccccttct cggcctcggg agggtggggg cgcgcgggcc
     5641 acttgcaccc caagggccgg gagctggacc ctgccgcgcc gcccgagggc cagctgctcc
     5701 gggaggtgcg cgcgctcggg gtccccttcg tccctcgcac cagcgtggat gcatggctgg
     5761 tgcacagcgt ggctgccggg agcgcggacg aggcccacgg gctgctcggc gccgccgccg
     5821 cctcgtccac cggaggagcc ggcgccagcg tggacggcgg cagccaggct gtgcaggggg
     5881 gcggcgggga cccccgagcg gctcggagtg gccccttgga cgccggggaa gaggagaagg
     5941 cacccgcgga accgacggct caggtgccgg acgctggcgg atgtgcgagc gaggagaatg
     6001 gggtactaag agaaaagcac gaagctgtgg atcatagttc ccagcatgag gaaaatgaag
     6061 aaagggtgtc agcccagaag gagaactcac ttcagcagaa tgatgatgat gaaaacaaaa
     6121 tagcagagaa acctgactgg gaggcagaaa agaccactga atctagaaat gagagacatc
     6181 tgaatgggac agatacttct ttctctctgg aagacttatt ccagttgctt tcatcacagc
     6241 ctgaaaattc actggagggc atctcattgg gagatattcc tcttccaggc agtatcagtg
     6301 atggcatgaa ttcttcagca cattatcatg taaacttcag ccaggctata agtcaggatg
     6361 tgaatcttca tgaggccatc ttgctttgtc ccaacaatac atttagaaga gatccaacag
     6421 caaggacttc acagtcacaa gaaccatttc tgcagttaaa ttctcatacc accaatcctg
     6481 agcaaaccct tcctggaact aatttgacag gatttctttc accggttgac aatcatatga
     6541 ggaatctaac aagccaagac ctactgtatg accttgacat aaatatattt gatgagataa
     6601 acttaatgtc attggccaca gaagacaact ttgatccaat cgatgtttct cagctttttg
     6661 atgaaccaga ttctgattct ggcctttctt tagattcaag tcacaataat acctctgtca
     6721 tcaagtctaa ttcctctcac tctgtgtgtg atgaaggtgc tataggttat tgcactgacc
     6781 atgaatctag ttcccatcat gacttagaag gtgctgtagg tggctactac ccagaaccca
     6841 gtaagctttg tcacttggat caaagtgatt ctgatttcca tggagatctt acatttcaac
     6901 acgtatttca taaccacact taccacttac agccaactgc accagaatct acttctgaac
     6961 cttttccgtg gcctgggaag tcacagaaga taaggagtag ataccttgaa gacacagata
     7021 gaaacttgag ccgtgatgaa cagcgtgcta aagctttgca tatccctttt tctgtagatg
     7081 aaattgtcgg catgcctgtt gattctttca atagcatgtt aagtagatat tatctgacag
     7141 acctacaagt ctcacttatc cgtgacatca gacgaagagg gaaaaataaa gttgctgcgc
     7201 agaactgtcg taaacgcaaa ttggacataa ttttgaattt agaagatgat gtatgtaact
     7261 tgcaagcaaa gaaggaaact cttaagagag agcaagcaca atgtaacaaa gctattaaca
     7321 taatgaaaca gaaactgcat gacctttatc atgatatttt tagtagatta agagatgacc
     7381 aaggtaggcc agtcaatccc aaccactatg ctctccagtg tacccatgat ggaagtatct
     7441 tgatagtacc caaagaactg gtggcctcag gccacaaaaa ggaaacccaa aagggaaaga
     7501 gaaaggcacc aactttcttg tacaaagtgg gcggcaagcc catccccaac cccctgctgg
     7561 gcctggacag caccgctagc atcccgctgc tgaacgctaa acagattctg ggacagctgg
     7621 acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc
     7681 gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag
     7741 gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt
     7801 ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg
     7861 cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt
     7921 atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg
     7981 ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg
     8041 tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac aggaatactc
     8101 tggccgctat gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc
     8161 tggctccata tctgtcacgg tgggagaagc tggataactt catcaataga cccgtgaagc
     8221 tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc
     8281 tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt
     8341 ctgccgaaaa gtaagccaac tttattatac atagttgata tccagcacag tggcggccgc
     8401 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8461 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8521 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8581 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8641 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8701 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8761 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     8821 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     8881 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8941 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     9001 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     9061 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     9121 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     9181 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     9241 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     9301 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     9361 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     9421 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     9481 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     9541 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     9601 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     9661 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     9721 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     9781 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     9841 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     9901 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     9961 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
    10021 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
    10081 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
    10141 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct
    10201 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    10261 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
    10321 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10381 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10441 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10501 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
    10561 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10681 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
    10741 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10801 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10861 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
    10921 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    10981 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    11041 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
    11101 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    11161 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    11221 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    11281 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    11341 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    11401 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    11461 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    11521 tacaattt
//
