LOCUS       sequence-417849-       10027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To block the formation of the initial Schiff base with FBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng
            JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, 
            Lin SC
  TITLE     Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by 
            AMPK.
  JOURNAL   Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub
            2017 Jul 19.
  PUBMED    28723898
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature23275"; journalName: "Nature"; date: "2017-08-3- 3"; 
            volume: "548"; issue: "7665"; pages: "112-116"
COMMENT     Sequence Label: pBoBi-hALDOA K230A
FEATURES             Location/Qualifiers
     source          1..10027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2157..2273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2296..2599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2600..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2800..2824
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2902..2928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2935..2961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2965..2991
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4194..4423
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4406..4425
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4600..5188
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4653..4673)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5071..5082)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5595..5775
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5801..5818)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5807..6031
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6077..6505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6164..6183)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6374..6395
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6514..6534)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6519..6848
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6699..6834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6761..6780
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6896..6943
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6962..7336
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7466..7587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7503..7522)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7557..7576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7636..7652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7636..7652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7649..7671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7660..7676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7684..7690,7691..7708,7709..7714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7729..7750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7867..7884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8038..8626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8118..8137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8797..9588,9589..9657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9420..9439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9658..9762)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9837..9856)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcaactttt
     2161 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2221 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata
     2281 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2341 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2401 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2461 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2521 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2581 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2641 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2701 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2761 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2821 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactgagctc
     2881 cttaaggtta acgccaccat gtacccttat gacgtacctg actatgctgg cgcctaccct
     2941 tatgacgtac ctgactatgc tggataccct tatgacgtac ctgactatgc tggatctaga
     3001 gaattcggat ccatgcccta ccaatatcca gcactgaccc cggagcagaa gaaggagctg
     3061 tctgacatcg ctcaccgcat cgtggcacct ggcaagggca tcctggctgc agatgagtcc
     3121 actgggagca ttgccaagcg gctgcagtcc attggcaccg agaacaccga ggagaaccgg
     3181 cgcttctacc gccagctgct gctgacagct gacgaccgcg tgaacccctg cattgggggt
     3241 gtcatcctct tccatgagac actctaccag aaggcggatg atgggcgtcc cttcccccaa
     3301 gttatcaaat ccaagggcgg tgttgtgggc atcaaggtag acaagggcgt ggtccccctg
     3361 gcagggacaa atggcgagac taccacccaa gggttggatg ggctgtctga gcgctgtgcc
     3421 cagtacaaga aggacggagc tgacttcgcc aagtggcgtt gtgtgctgaa gattggggaa
     3481 cacaccccct cagccctcgc catcatggaa aatgccaatg ttctggcccg ttatgccagt
     3541 atctgccagc agaatggcat tgtgcccatc gtggagcctg agatcctccc tgatggggac
     3601 catgacttga agcgctgcca gtatgtgacc gagaaggtgc tggctgctgt ctacaaggct
     3661 ctgagtgacc accacatcta cctggaaggc accttgctgg cgcccaacat ggtcacccca
     3721 ggccatgctt gcactcagaa gttttctcat gaggagattg ccatggcgac cgtcacagcg
     3781 ctgcgccgca cagtgccccc cgctgtcact gggatcacct tcctgtctgg aggccagagt
     3841 gaggaggagg cgtccatcaa cctcaatgcc attaacaagt gccccctgct gaagccctgg
     3901 gccctgacct tctcctacgg ccgagccctg caggcctctg ccctgaaggc ctggggcggg
     3961 aagaaggaga acctgaaggc tgcgcaggag gagtatgtca agcgagccct ggccaacagc
     4021 cttgcctgtc aaggaaagta cactccgagc ggtcaggctg gggctgctgc cagcgagtcc
     4081 ctcttcgtct ctaaccacgc ctattaactc gagccatgga agcttgatat ctaactgact
     4141 gaaccggtgg taccgaggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta
     4201 ctccctctca aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga
     4261 ggatttgata ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc
     4321 agaaaagaca atctttttgt tgtcaagctt gaggtgtggc aggcttgaga tctggccata
     4381 cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc
     4441 caactgcagg tcgagcatgc atctagggcg gccaattccg ccgatcctgg aaaaacatgg
     4501 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4561 agaggaggag gaggtgggtt ttccagtcac acctcagaca atcaacctct ggattacaaa
     4621 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4681 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4741 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4801 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4861 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4921 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4981 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     5041 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5101 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5161 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa
     5221 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5281 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     5341 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     5401 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     5461 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     5521 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     5581 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5641 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5701 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5761 ggaaaatctc tagcagggcc gctttaaacc cgctgatcag cctcgactgt gccttctagt
     5821 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5881 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5941 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6001 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6061 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6121 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6181 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6241 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6301 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6361 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6421 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6481 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6601 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6661 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6721 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6781 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6841 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     6901 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     6961 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     7021 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     7081 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     7141 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     7201 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     7261 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     7321 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7381 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7441 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7501 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7561 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7621 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7681 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8581 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8641 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8701 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9661 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9721 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9781 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9841 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     9901 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     9961 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    10021 acgcgtt
//
