LOCUS       sequence-417850-       10024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For stably expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng
            JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, 
            Lin SC
  TITLE     Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by 
            AMPK.
  JOURNAL   Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub
            2017 Jul 19.
  PUBMED    28723898
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature23275"; journalName: "Nature"; date: "2017-08-3- 3"; 
            volume: "548"; issue: "7665"; pages: "112-116"
COMMENT     Sequence Label: pBoBi-hALDOA
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2157..2273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2296..2599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2600..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2800..2824
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2902..2928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2935..2961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2965..2991
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4191..4420
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4403..4422
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4597..5185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4650..4670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5068..5079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5592..5772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5798..5815)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5804..6028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6074..6502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6161..6180)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6371..6392
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6511..6531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6516..6845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6696..6831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6758..6777
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6893..6940
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6959..7333
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7463..7584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7500..7519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7554..7573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7633..7649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7633..7649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7646..7668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7657..7673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7681..7687,7688..7705,7706..7711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7726..7747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7864..7881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8035..8623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8115..8134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8794..9585,9586..9654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9417..9436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9655..9759)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9834..9853)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcaactttt
     2161 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2221 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata
     2281 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2341 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2401 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2461 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2521 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2581 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2641 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2701 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2761 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2821 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactgagctc
     2881 cttaaggtta acgccaccat gtacccttat gacgtacctg actatgctgg cgcctaccct
     2941 tatgacgtac ctgactatgc tggataccct tatgacgtac ctgactatgc tggatctaga
     3001 gaattcggat ccatgcccta ccaatatcca gcactgaccc cggagcagaa gaaggagctg
     3061 tctgacatcg ctcaccgcat cgtggcacct ggcaagggca tcctggctgc agatgagtcc
     3121 actgggagca ttgccaagcg gctgcagtcc attggcaccg agaacaccga ggagaaccgg
     3181 cgcttctacc gccagctgct gctgacagct gacgaccgcg tgaacccctg cattgggggt
     3241 gtcatcctct tccatgagac actctaccag aaggcggatg atgggcgtcc cttcccccaa
     3301 gttatcaaat ccaagggcgg tgttgtgggc atcaaggtag acaagggcgt ggtccccctg
     3361 gcagggacaa atggcgagac taccacccaa gggttggatg ggctgtctga gcgctgtgcc
     3421 cagtacaaga aggacggagc tgacttcgcc aagtggcgtt gtgtgctgaa gattggggaa
     3481 cacaccccct cagccctcgc catcatggaa aatgccaatg ttctggcccg ttatgccagt
     3541 atctgccagc agaatggcat tgtgcccatc gtggagcctg agatcctccc tgatggggac
     3601 catgacttga agcgctgcca gtatgtgacc gagaaggtgc tggctgctgt ctacaaggct
     3661 ctgagtgacc accacatcta cctggaaggc accttgctga agcccaacat ggtcacccca
     3721 ggccatgctt gcactcagaa gttttctcat gaggagattg ccatggcgac cgtcacagcg
     3781 ctgcgccgca cagtgccccc cgctgtcact gggatcacct tcctgtctgg aggccagagt
     3841 gaggaggagg cgtccatcaa cctcaatgcc attaacaagt gccccctgct gaagccctgg
     3901 gccctgacct tctcctacgg ccgagccctg caggcctctg ccctgaaggc ctggggcggg
     3961 aagaaggaga acctgaaggc tgcgcaggag gagtatgtca agcgagccct ggccaacagc
     4021 cttgcctgtc aaggaaagta cactccgagc ggtcaggctg gggctgctgc cagcgagtcc
     4081 ctcttcgtct ctaaccacgc ctatctcgag ccatggaagc ttgatatcta actgactgaa
     4141 ccggtggtac cgaggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
     4201 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     4261 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
     4321 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
     4381 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     4441 ctgcaggtcg agcatgcatc tagggcggcc aattccgccg atcctggaaa aacatggagc
     4501 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     4561 ggaggaggag gtgggttttc cagtcacacc tcagacaatc aacctctgga ttacaaaatt
     4621 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4801 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4861 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4921 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4981 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5041 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5101 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5161 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     5221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5281 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     5341 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     5401 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     5461 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     5521 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     5581 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5641 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5701 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5761 aaatctctag cagggccgct ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5821 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5881 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5941 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6001 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     6061 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6121 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6181 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6241 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6301 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6361 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6421 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6481 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6541 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6601 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6661 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6721 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6781 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6841 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     6901 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     6961 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     7021 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     7081 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     7141 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     7201 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     7261 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     7321 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     7381 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7441 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7501 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7561 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7621 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7681 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7741 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7801 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7861 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7921 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8221 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8461 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9781 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     9841 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     9901 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     9961 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
    10021 cgtt
//
