LOCUS       sequence-417851-        9251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GRM2-Tango
FEATURES             Location/Qualifiers
     source          1..9251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3575..4189,4190..4576)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3642..3661)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4608..4625)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4614..4838
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4884..5312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4971..4990)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5181..5202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5321..5341)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5326..5655
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5506..5641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5568..5587
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5722..6516
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5776..5795)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6386..6405
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6690..6811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6727..6746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6781..6800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6860..6876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6860..6876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6873..6895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6884..6900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6908..6914,6915..6932,6933..6938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6953..6974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7091..7108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7262..7850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7342..7361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8021..8812,8813..8881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8644..8663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8882..8986)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9061..9080)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 gggcagtttg cttgctctct tggctctttt gttgttgtgg ggggctgtcg cggagggacc
      901 tgccaagaaa gtgctgaccc ttgaggggga cctggtcctt ggcggactgt tccccgtgca
      961 ccaaaaaggt ggacccgccg aagactgcgg gccggttaac gagcatcgag gcatccagcg
     1021 gctcgaagcg atgctcttcg ctctcgatag aattaatcgg gatccccatc tgcttccagg
     1081 cgtgcggctt ggcgcacata ttctggattc atgctccaaa gatactcatg ctctcgaaca
     1141 agcactcgac ttcgtgcgag ctagcctctc ccgcggcgcc gatggttcaa gacacatctg
     1201 ccccgatgga tcctacgcca cgcacggtga cgcccccacc gctattacgg gggtgattgg
     1261 gggtagctat agtgatgtta gcatacaggt ggccaatttg ctccgcctgt tccagatccc
     1321 ccagattagc tatgcctcaa cctcagccaa actgtccgat aagagtaggt atgactactt
     1381 cgcccgcaca gtgcctccag atttctttca agccaaggct atggcagaga tactccgctt
     1441 ctttaattgg acatacgttt ctactgtagc cagcgaagga gactacgggg agactggcat
     1501 cgaggcgttc gaactcgaag ctagagcgag aaacatatgt gtcgccacct ccgaaaaggt
     1561 cgggcgggca atgtcacgcg ctgcctttga gggagtcgtt cgagccctgc tgcagaagcc
     1621 ttccgcaagg gttgcagtcc tgtttacgcg cagtgaggat gcgcgcgaat tgctggccgc
     1681 ttctcaacga ctgaacgcat cctttacatg ggttgccagt gacgggtggg gcgctctgga
     1741 aagtgtggtg gccggaagtg agggagcggc agagggggca attacgatcg aactggctag
     1801 ctaccccatc agcgattttg cctcctactt ccagtccctg gacccatgga acaacagccg
     1861 caatccttgg ttcagagaat tttgggagca gcggtttagg tgtagcttcc gacagcggga
     1921 ctgtgccgct cactcattga gagccgtccc ttttgagcag gagtccaaga tcatgttcgt
     1981 cgtcaatgca gtttacgcca tggcccacgc gctccataat atgcaccggg ccctgtgccc
     2041 taacaccaca cgcctgtgcg atgctatgag gcctgtcaac ggacgaaggc tgtataagga
     2101 cttcgtcctg aacgtgaagt tcgacgctcc ctttcggccc gccgatacac ataatgaggt
     2161 gcgcttcgac cgcttcgggg acgggattgg aagatacaac atcttcacgt acctgagagc
     2221 cggatcaggc cgctacagat accagaaagt gggttactgg gccgagggcc tgaccctgga
     2281 caccagtttg atcccctggg ctagcccttc tgcagggccc ttgccggcat ctcggtgctc
     2341 agagccctgt ttgcagaacg aggtaaaaag tgttcagccc ggggaagtgt gttgttggct
     2401 gtgcatcccc tgtcagccat acgagtatcg cctggatgag ttcacgtgtg cagattgcgg
     2461 cctcggctat tggcccaacg ccagcctgac tggctgcttc gagcttcccc aggagtatat
     2521 ccgatggggc gacgcctggg ctgtaggccc tgtgacaata gcttgcctcg gggctctggc
     2581 aaccctgttc gtgctgggag ttttcgttag acacaacgcc acacccgtag tcaaggcctc
     2641 tggccgcgaa ctctgctaca tcttgctggg gggggtgttc ctttgctatt gcatgacttt
     2701 catctttatt gctaaacctt caacagccgt gtgcactctc agaaggctgg ggctcggcac
     2761 agcgttctca gtgtgttact cagccttgtt gaccaaaacc aaccgcattg ccaggatttt
     2821 tggcggtgca agagagggcg cccagaggcc aagatttatt tcccctgctt cacaggtggc
     2881 catatgcctg gccttgattt ccggacagct tttgattgtc gtggcatggc tggtagtgga
     2941 ggcccctggg actggaaagg agacagcgcc tgaacgaagg gaggtggtca ccctccgctg
     3001 caatcatcgg gacgcctcta tgctcggcag tctggcttat aatgttcttt tgatcgcgct
     3061 ctgcactctt tacgcgttca agacacggaa atgcccagaa aacttcaacg aagcaaaatt
     3121 catcggcttc actatgtaca caacgtgtat tatctggttg gcattcctgc ctatatttta
     3181 cgtcacctct agcgactatc gggtacagac taccactatg tgtgtgagcg tgtctctgag
     3241 tgggtctgtt gtgcttggct gtctgtttgc accaaagctg catattatcc tgttccagcc
     3301 ccaaaagaac gtggtcagtc atcgggcccc gaccagcagg tttggtagtg cagccgctcg
     3361 cgcttctagc tcacttggcc aaggctctgg cagccaattc gtgcccaccg tgtgcaatgg
     3421 ccgagaagtg gtagacagta caaccagtag tctcatcgat accggtggac gcaccccacc
     3481 cagcctgggt ccccaagatg agtcctgcac caccgccagc tcctccctgg ccaaggacac
     3541 ttcatcgacc ggtgagaacc tgtacttcca gctaagatta gataaaagta aagtgattaa
     3601 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     3661 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     3721 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     3781 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     3841 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     3901 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     3961 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
     4021 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     4081 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
     4141 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagccg
     4201 cgcgcgtacg aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga
     4261 cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca
     4321 cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     4381 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     4441 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     4501 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     4561 cgagtacggt gggtagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4621 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4681 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4741 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4801 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4861 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4921 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4981 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5041 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5101 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5161 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5221 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5281 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5341 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5401 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5461 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5581 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5641 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     5701 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5761 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5821 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5881 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     5941 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6001 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6061 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6121 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6181 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6241 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6301 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6361 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6421 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6481 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     6541 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6601 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6661 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6721 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6781 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6841 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7021 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7681 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8881 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8941 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9001 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     9061 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     9121 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     9181 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     9241 atatacgcgt t
//
