LOCUS       sequence-417944-       10590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that expresses diptheria toxin receptor (DTR) 
            C-terminally fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Fernandez Moro C, Geyer N, Harrizi S, Hamidi Y, Soderqvist S, 
            Kuznyecov D, Tidholm Qvist E, Salmonson Schaad M, Hermann L, 
            Lindberg A, Heuchel RL, Martin-Bernabe A, Dhanjal S, Navis AC, 
            Villard C, Del Valle AC, Bozoky L, Sparrelid E, Dirix L, Strell C, 
            Ostman A, Schmierer B, Vermeulen PB, Engstrand J, Bozoky B, Gerling 
            M
  TITLE     An idiosyncratic zonated stroma encapsulates desmoplastic liver 
            metastases and originates from injured liver.
  JOURNAL   Nat Commun. 2023 Aug 18;14(1):5024. doi: 10.1038/s41467-023-40688-x.
  PUBMED    37596278
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-18"; volume: "14(1):5024. doi"; pages: " 
            10.1038/s41467-023-40688-x"
COMMENT     Sequence Label: lenti.DTR.GFP
FEATURES             Location/Qualifiers
     source          1..10590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(99..118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(778..1569,1570..1638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1401..1420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1639..1743)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1818..1837)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2009..2388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2390..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2543..2563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2606..2786
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2833..2958
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3451..3684
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3869..3913
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4211..4328
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4458..5636
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4689..5627
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5584..5604
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6295..6304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6301..6303,6304..6306,6307..7020)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6301..6303,6304..6306,6307..7017)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6346..6367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6607..6626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6954..6975
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7053..7641
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7106..7126)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7524..7535)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7644..7660)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7645..7661)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8166..8346
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8372..8389)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8378..8602
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8648..9076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8735..8754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8945..8966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9085..9105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9090..9419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9270..9405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9332..9351
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9467..9514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9533..9907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10037..10158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10074..10093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10128..10147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10207..10223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10207..10223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10220..10242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10231..10247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10255..10261,10262..10279,10280..10285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10300..10321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10438..10455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
       61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1741 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     1801 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     1861 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     1921 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     1981 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     2041 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2101 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2161 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2221 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2281 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2341 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2401 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2461 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2521 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2581 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     2641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2761 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2821 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2881 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2941 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     3001 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     3061 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     3121 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     3181 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     3241 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     3301 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     3361 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     3421 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     3481 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     3541 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     3601 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     3661 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3721 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3781 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3841 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3901 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3961 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     4021 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     4081 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     4141 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     4201 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     4261 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     4321 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt agctagctgc
     4381 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     4441 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     4501 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     4561 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     4621 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     4681 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     4741 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     4801 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     4861 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     4921 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     4981 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     5041 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     5101 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     5161 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     5221 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     5281 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     5341 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     5401 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     5461 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     5521 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     5581 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacctg
     5641 caggaattcg ccactatgaa gctgctgccg tcggtggtgc tgaagctcct tctggctgca
     5701 gttctttcgg cactggtgac tggcgagagc ctggagcagc ttcggagagg gctagctgct
     5761 ggaaccagca acccggaccc ttccactgga tctacggacc agctgctacg cctaggaggc
     5821 ggccgggacc ggaaagtccg tgacttgcaa gaggcagatc tggacctttt gagagtcact
     5881 ttatcctcca agccacaagc actggccaca ccaagcaagg aggagcacgg gaaaagaaag
     5941 aagaaaggca agggactagg gaagaagagg gacccatgtc ttcggaaata caaggacttc
     6001 tgcatccacg gagaatgcaa atatgtgaag gagctccggg ctccctcctg catctgccac
     6061 ccaggttacc atggagagag gtgtcatggg ctgagcctcc cagtggaaaa tcgcttatat
     6121 acctatgacc atacaactat cctggctgtg gtggccgtgg tgctgtcctc tgtctgtctg
     6181 ctggtcatcg tggggcttct catgtttagg taccatagga gaggtggtta tgatgtggaa
     6241 aacgaagaga aagtgaagtt gggcatgact aattcccacc cggatccacc ggtcgccacc
     6301 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6361 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6481 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6541 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6601 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6661 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6721 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6781 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6841 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6901 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6961 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     7021 agcggccgga attcgatatc aagcttatcg gtaatcaacc tctggattac aaaatttgtg
     7081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7201 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7261 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7321 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7381 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7441 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7501 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7561 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7621 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     7681 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     7741 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     7801 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7861 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     7921 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     7981 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     8041 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     8101 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     8161 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8221 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8281 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8341 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     8401 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8461 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8521 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8581 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     8641 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8941 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9001 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9061 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     9121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     9181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     9301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     9361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9421 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     9481 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     9541 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     9601 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     9661 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     9721 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     9781 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     9841 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     9901 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
    10021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
    10201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10561 aggccagcaa aaggccagga accgtaaaaa
//
