LOCUS       sequence-417946-       11605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 
            2-468)-gp41-1_IntN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Zeng H, Yuan Q, Peng F, Ma D, Lingineni A, Chee K, Gilberd P, Osikpa
            EC, Sun Z, Gao X
  TITLE     A split and inducible adenine base editor for precise in vivo base 
            editing.
  JOURNAL   Nat Commun. 2023 Sep 11;14(1):5573. doi: 10.1038/s41467-023-41331-5.
  PUBMED    37696818
REFERENCE   2  (bases 1 to 11605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-11"; volume: "14(1):5573. doi"; pages: " 
            10.1038/s41467-023-41331-5"
COMMENT     Sequence Label: sABE v3.22 Lenti_1
FEATURES             Location/Qualifiers
     source          1..11605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             32..212
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    259..384
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    877..1110
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1295..1339
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1637..1754
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1820..2199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2200..2403
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2434..2454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5293..5317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5351..5939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5404..5424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5822..5833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5942..5958)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5943..5959)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6464..6644
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6670..6687)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6676..6900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6946..7374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7033..7052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7243..7264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7383..7403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7388..7717
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7568..7703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7630..7649
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7765..7812
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7831..8205
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8335..8456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8372..8391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8426..8445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8505..8521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8505..8521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8518..8540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8529..8545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8553..8559,8560..8577,8578..8583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8598..8619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8736..8753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8907..9495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8987..9006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9572..10363)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(10849..10868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11040..11419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(11421..11605,1..14)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gtctatataa gcagcgcgtt ttgcctgtac tgggtgtctc tggttagacc agatctgagc
       61 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      121 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      181 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      241 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      301 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
      361 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
      421 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
      481 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
      541 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
      601 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
      661 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
      721 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
      781 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
      841 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
      901 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
      961 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1021 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1081 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1141 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1201 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1261 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1321 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1381 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1441 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1501 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1561 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     1621 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1681 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1741 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaact
     1801 ccggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1861 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1921 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1981 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2041 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2101 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2161 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2221 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2281 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2341 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2401 gctctctggc taactagaga acccactgct atgccaaaga aaaagaggaa ggtgagcccc
     2461 aagtacacca agagcgtgct gaagaagggt gacaagacca acttccccaa aaagggagat
     2521 gtggtgcact gctggtacac cggcacgctg caagacggca ccgtgttcga caccaacatc
     2581 cagaccagcg ctaaaaagaa gaaaaacgcc aaacccctgt ctttcaaagt gggcgtgggc
     2641 aaggtgatca ggggctggga cgaagctctg ctgaccatga gcaagggcga gaaggccagg
     2701 ctggagatcg agcccgagtg ggcctatggc aagaaaggac agccagacgc caagatcccg
     2761 cccaacgcaa aactgacctt tgaggtggaa ctggttgata tcgatggtgg cggctcaggt
     2821 ggcgggagcg gaggcgggag ctctcccaag tacaccaaga gtgttctgaa gaaaggcgac
     2881 aagaccaact tccccaaaaa gggagatgtg gtgcactgct ggtacaccgg cacgctgcaa
     2941 gacggcaccg tgttcgacac caacatccag accagcgcta aaaagaagaa aaacgccaaa
     3001 cccctgtctt tcaaagtggg cgtgggcaag gtgatcaggg gctgggacga agctctgctg
     3061 accatgagca agggcgagaa ggccaggctg gagatcgagc ccgagtgggc ctatggcaag
     3121 aaaggacagc cagacgccaa gatcccgccc aacgcaaaac tgacctttga ggtggaactg
     3181 gttgatatcg atggtggagg ctctggagga agcagcggcg gcagctcggg cgacgccacc
     3241 ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc
     3301 ggccgcgtgg tgtttggcgt gaggaactca aaaagaggcg ccgcaggctc cctgatgaac
     3361 gtgctgaact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat
     3421 gaatgtgccg ccctgctgtg cgatttctat cggatgccta gacaggtgtt caatgctcag
     3481 aagaaggccc agagctccat caactccgga ggatctagcg gaggatcctc tggcagcgag
     3541 acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc
     3601 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     3661 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     3721 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     3781 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     3841 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     3901 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     3961 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     4021 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     4081 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     4141 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     4201 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     4261 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     4321 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     4381 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     4441 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     4501 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     4561 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     4621 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     4681 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     4741 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     4801 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     4861 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     4921 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     4981 ttcgcctgga tgaccagaaa gtgcctggac ctgaaaaccc aggtgcagac ccctcagggc
     5041 atgaaggaga taagcaacat ccaggtcggc gaccttgttc tgagcaacac cggctataac
     5101 gaggtgctga atgtatttcc caagagcaag aagaagagct acaagatcac cctggaggac
     5161 ggcaaggaaa tcatctgcag cgaggagcac ctgttcccca cccagactgg cgagatgaac
     5221 atcagcggag gactgaagga aggaatgtgc ctgtatgtaa aggagtaaac taggatccta
     5281 gataactgat ctacccagct ttcttgtaca aagtggtgaa ttccggcaat tcgatatcaa
     5341 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5401 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5461 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5521 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5581 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5641 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5701 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5761 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5821 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5881 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5941 tcgataccgt cgacctcgag tcctagaaaa acatggagca atcacaagta gcaatacagc
     6001 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6061 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6121 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6181 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     6241 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6301 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     6361 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     6421 agcatttcat cacatggccc gagagctgca tccggactgt actgggtgtc tctggttaga
     6481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     6661 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6721 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6781 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6841 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6901 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6961 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7021 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7081 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7141 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7201 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7261 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7321 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7381 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7441 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7501 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7621 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7681 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7741 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7801 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7861 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7921 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7981 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     8041 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8101 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8161 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8221 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8281 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8341 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8401 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8461 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8521 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8581 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8641 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8701 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8761 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8821 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9541 gaaaactcac gttaagggat tttggtcatg attatttgcc gactaccttg gtgatctcgc
     9601 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     9661 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     9721 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     9781 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     9841 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     9901 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     9961 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
    10021 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
    10081 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
    10141 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
    10201 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
    10261 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
    10321 ctgatagttg agtcgatact tcggcgatca ccgcttccct cataatgttt aactttgttt
    10381 tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
    10441 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt
    10501 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
    10561 tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt
    10621 atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
    10681 gtagcagcga agtcgaggca tttctgtcct ggctggtcat gagcggatac atatttgaat
    10741 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10801 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    10861 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
    10921 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
    10981 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
    11041 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
    11101 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
    11161 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
    11221 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    11281 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
    11341 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
    11401 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
    11461 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
    11521 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
    11581 gggcggtagg cgtgtacggt gggag
//
