LOCUS       sequence-417949-       10367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Zeng H, Yuan Q, Peng F, Ma D, Lingineni A, Chee K, Gilberd P, Osikpa
            EC, Sun Z, Gao X
  TITLE     A split and inducible adenine base editor for precise in vivo base 
            editing.
  JOURNAL   Nat Commun. 2023 Sep 11;14(1):5573. doi: 10.1038/s41467-023-41331-5.
  PUBMED    37696818
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-11"; volume: "14(1):5573. doi"; pages: " 
            10.1038/s41467-023-41331-5"
COMMENT     Sequence Label: sABE v3.22 Lenti_2
FEATURES             Location/Qualifiers
     source          1..10367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             18..198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    245..370
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    863..1096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1281..1325
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1623..1740
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1818..2066
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1818..1838
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1989..2008
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2087..2162
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2604..2807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2849..2868
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2849..2867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2917..2937
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(3837..3855)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3838..3855)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(3914..3938)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3972..4560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4025..4045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4443..4454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4563..4579)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4564..4580)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5085..5265
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5424..5648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5694..6122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5781..5800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5991..6012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6131..6151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6136..6465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6316..6451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6378..6397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6513..6560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6579..6953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7083..7204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7120..7139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7174..7193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7253..7269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7253..7269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7266..7288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7277..7293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7301..7307,7308..7325,7326..7331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7346..7367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7484..7501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7655..8243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7735..7754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8320..9111)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(9597..9616)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9788..10167
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10169..10367
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 cgcgttttgc ctgtactggg tgtctctggt tagaccagat ctgagcctgg gagctctctg
       61 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      121 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      181 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      241 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      301 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      361 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      421 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      481 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      541 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      601 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      661 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
      721 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
      781 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
      841 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
      901 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
      961 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1021 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1081 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1141 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1201 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1261 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1321 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1381 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1441 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     1501 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     1561 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     1621 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1681 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1741 cgggtttatt acagggacag cagagatcca gtttggttaa ttaactcccc cacctcccaa
     1801 ccccgagggg acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     1861 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     1921 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     1981 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     2041 atatcttgtg gaaaggacga aacaccgaac acaaagcata gactgcgttt tagagctaga
     2101 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2161 gcttttttaa gcttggcgta actagatctt gagattcgcg ctcccggccc atctcaacgc
     2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat tgagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     2821 gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc
     2881 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct
     2941 gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca
     3001 cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc
     3061 gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc
     3121 ctgagacagg gcggcctggt catgcagaac tacagactga ttggaggctc tggaggaagc
     3181 agcggcggca gctcgggcgg tggcggctcg agcatcctgt ggcatgagat gtggcacgag
     3241 ggcctggagg aggccagcag gctgtatttc ggcgagagga atgtgaaggg catgttcgag
     3301 gtgctggagc ccctgcacgc catgatggaa aggggcccac agaccctgaa ggaaactagc
     3361 ttcaaccagg cctacggcag ggacctgatg gaggcccagg agtggtgcag gaagtacatg
     3421 aagagcggca acgtgaagga tctgacccag gcttgggact tgtactacca cgtgttcagg
     3481 aggatcagct cgggcggtgg cggctcgagc atcctgtggc atgagatgtg gcacgagggc
     3541 ctggaggagg ccagcaggct gtatttcggc gagaggaatg tgaagggcat gttcgaggtg
     3601 ctggagcccc tgcacgccat gatggaaagg ggcccacaga ccctgaagga aactagcttc
     3661 aaccaggcct acggcaggga cctgatggag gcccaggagt ggtgcaggaa gtacatgaag
     3721 agcggcaacg tgaaggatct gacccaggct tgggacttgt actaccacgt gttcaggagg
     3781 atcagctaaa ctacgaaccc cgggtaaatg catgaattcg atctagaggg ccctattcta
     3841 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgcctta ctaggatcct
     3901 agataactga tctacccagc tttcttgtac aaagtggtga attccggcaa ttcgatatca
     3961 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4021 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4081 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4141 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4201 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4261 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4321 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4381 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4441 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4501 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4561 atcgataccg tcgacctcga gtcctagaaa aacatggagc aatcacaagt agcaatacag
     4621 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     4681 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4741 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     4801 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     4861 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     4921 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     4981 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     5041 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtgt ctctggttag
     5101 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5161 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5221 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagaccc actgcttaag
     5281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     5401 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5581 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5641 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5701 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5761 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5821 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5881 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5941 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6001 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6061 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6121 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6181 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6481 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     6541 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     6601 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     6661 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     6721 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     6781 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     6841 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     6901 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     6961 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7021 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7081 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7201 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8281 agtggaacga aaactcacgt taagggattt tggtcatgat tatttgccga ctaccttggt
     8341 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     8401 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     8461 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     8521 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     8581 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     8641 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     8701 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     8761 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     8821 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     8881 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     8941 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     9001 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     9061 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca taatgtttaa
     9121 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     9181 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     9241 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     9301 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaccga
     9361 acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     9421 aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggtcatga gcggatacat
     9481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9541 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9601 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     9661 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     9721 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     9781 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     9841 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9901 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9961 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
    10021 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    10081 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    10141 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
    10201 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
    10261 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
    10321 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcag
//
