LOCUS       sequence-417950-        8948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3-SREBP-2 (A1134-1135)
FEATURES             Location/Qualifiers
     source          1..8948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(274..603)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      288..423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(342..361)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     588..608
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(617..1045)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(727..748)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     939..958
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1091..1315
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1304..1321
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4839..4871)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(4872..4904)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(4905..4937)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     regulatory      4934..4943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4978..4997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4979..4997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5042..5245)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5071..5091)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5246..5625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5797..5816
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5891..5995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5996..6064,6065..6856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6214..6233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7027..7615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7516..7535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7769..7786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7903..7924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7939..7944,7945..7962,7963..7969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7977..7993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7982..8004
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8001..8017
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8001..8017
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8066..8187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8077..8096)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8131..8150
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(8361..8948,1..207))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8472..8491)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
       61 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
      121 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
      181 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
      241 tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa
      301 gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat
      361 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
      421 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      481 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      541 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      601 cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      661 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      721 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      781 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      841 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      901 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      961 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1021 cttaatgcgc cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc
     1081 gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
     1141 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
     1201 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
     1261 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
     1321 gctgatcagc gggtttaaac gggccctcta gactcgagcg gccgccactg tgctggatat
     1381 ctgcagaatt cggatcctca ggaggcggca atggcagtgg cagcacccag cttaacaatc
     1441 atctgctggc agtcgttgca ggagcgccgg tcgcccacct tctccagggt gcgggcagct
     1501 tcggccagca gcactgcccg ctggcccggg gaggagagga aggagagggg caggtggcgg
     1561 caggccagca ggatggcggt ggcccgctct cgctggccgg gccaggcatc cacctctccg
     1621 tgcttggtgc tctgcgtggt gcgccgccgc aggctgtgtt ccagcagctg gtgggtgcgg
     1681 gtggggctgg ctcctgccat caggcgcacg gtggcttcat gcaggaacac cttgcggtat
     1741 gctgggcgga agctgtgtgc cagcctgcgc aggctgccca ggtcccgttg gaagcccgcc
     1801 agttcagcgc ctgacgcgtg gtaggtctcc cccacagcct ggctggcact ggcctgtttt
     1861 tgccagagcg ctgtccgtag cgacagtagc aggtcacagg tgagcagctg gaccacgtgg
     1921 ttgagggcag ggtcagaggt ggccccactg acgttgaggc tgctccatag gtggccactg
     1981 gccctctcgc aatggcagaa ggaactctgc tgcccatctg ctttcccagg gagtgaggca
     2041 tgcatggctc tgcaggcatg gaagatggcc ttcaccaggg ggctctctgt cacttccagg
     2101 gccttgggga tgcgttccac tttggtaaaa tgagagcgca cagctgcatc gtctccctgg
     2161 agccagctga tggccacagt gattgcagac gtccaccacc gacagatgat gtctggaccc
     2221 agggcggact tgagcacgga gctcctggag agtggggggc tcataacccc cacagagtcc
     2281 acaaaagaat gaagtaattt caagtactcc agagcactgg agaattcaca gctctcttct
     2341 tcctggtctc cagccttctt cttggcctga ggtttcacca aggactctat agctcgctcc
     2401 agcaggttct tgcagaaggc ctggtggacc tgcgcaatgg ggtcagctgg gttcctctgg
     2461 gcacagtata gactctcctt ggcagctgac ttcacagacc agctccgctc catgaaaaac
     2521 ttctggccca gggggtggca gagccagcgc agggagtcag gaacagcact gtgctcgggg
     2581 ccacacaggc tctgggctcg gctgaggaag tagctggcca ggaagcccag cttgcctcca
     2641 caccgggtct tgagccccat ggcagcagtc agatggatct caaccagtgt gctcggtggg
     2701 atcttctcct ctgcacattc agccaggttc acggcacaca acgccatgtg tacatcggaa
     2761 caggcggatc ctgcaggaag cttccctgtg atgtgcagct ggtgcagccg gtgataggcc
     2821 agagccgcat cccgggcgct ggtcttagct tcgtcttcaa agcctgcctc agtggctggc
     2881 gtggcccgcc ggcactggaa gactttcttg agcagccagc gcaccaggcg tagcttctgc
     2941 aggctgtagc ggatcacgtt ccaggagagg ctgcaggcca ggtccaggcg ggaggtgggc
     3001 agtgcccggc ccaaaactgc caggcaggtt tgtaggttgc cggcagcagc tgcaaaatct
     3061 cctctggcga gatccagatc tgcctgtttc cggtgcctcc agaaggtgac cgaggagcgc
     3121 gagtgtggcc ggatcactgg ctccccatga accagcagct tcacaaagac gctcaggaca
     3181 atcacaccat ttaccagcca taagagaaga gtaggcatca tccagtcaaa ccagccccca
     3241 gaacctgact cgaatgacag gacactgcgg ccagagcctg agtgtgggtg ctggtcagag
     3301 tcgtgggccc ctccccactg cagcagggaa gtcagggggt taaaggagag gcacaggaag
     3361 gtgaggacac acagaagaat ccgtgagcgg tctaccatgc ccagcgccac aggaggagag
     3421 tctggctcat ctttgacctt tgcatcatcc aatagagggc ttcctggctc agagtcaatg
     3481 gagtagggag agaagccagc ctgggaccct gagtcagagg ctggggggga catcagaagg
     3541 acattctgat taaagtcctc gatcttcagg tccacctcat tgtccaccag actgcctagg
     3601 tcgatgccct ttagaagctt gttcttttga tttgccagct tcagcaccat gttctcctgg
     3661 cgcagtttat gattgacctg ctgcaagtat ttgatgtaat caatggcctt cctcagaacg
     3721 ccagacttgt gcatcttggc gtctgtcccc atgaccaggt ctttcaattc gatgattttg
     3781 tcattgatgg aggagcgata tcgtttctca atgatattat gggttgtccg cctttctcct
     3841 tctttggggg gctcaagctg cttgactccc ccaggtacct gcttaatggg cactttctct
     3901 tgccccatca ttacaggcat tgtggtcaga atggtcccac tgctgcccac cagggttggt
     3961 acttgaaggg cagccgtctg gataggggtg gtgagggcgg tgagggccgg gttctggacc
     4021 gcagccataa cagggctgcc atctgtcttc agtgtggtca aaacaaggga atctgtcttg
     4081 atgatctgag gctggaccag gaccgggacc tgctgcacct gtggcgcagc aactgtctgc
     4141 accgtagccg gggcaagggt ctgcagcgtg ccattggccg tttgtgtcag cacccgctgg
     4201 gcctgtactg tctgcacctg ctgctgaatg gtgaccggct gtacctggga ggatgtcacc
     4261 aggctttgga cttgaggctg aaggacttga aagctagtag ctgcattctg gtatatcaaa
     4321 ggctgctgga tgatcctcgt ctgcggagtg gtgctgaatg ttggcgtgat cattaccgtc
     4381 tgttgttgca gctgagtttg aggttgaggc tggggctggg ggcggggctg aagaatagga
     4441 gttgccctgg gtgtggtggg aactgaggtg ggagaaacct tgacttgcag agttggagcc
     4501 tgtggggagg ccgccgaggg agagaaggaa ggtaatgtga cctgggtgaa tgaccgttgc
     4561 actgaagggt ccacagctcc gctgctgctg cccctgccat tgctgctgct gctgctgctg
     4621 ccactgctgc cgctgctgct accactacca ccactgccag gaaaggagct acacagctgt
     4681 tctgaaaaca agtcagggaa ctctcccact tgattactga caaattgcag catctcgtcg
     4741 atgtctccca gggtcagctc gtcgcccagc tccgtgaggg tctccatggt ctccagacca
     4801 cccagctcgc cgctgtcgtc accggtacct atcgatgcac ccatttgctg tccaccagtc
     4861 atgctagcca tacccatttg ctgtccacca gtcatgctag ccatacccat ttgctgtcca
     4921 ccagtcatgc tagccatggt ggcaagctta agtttaaacg ctagccagct tgggtctccc
     4981 tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga
     5041 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     5101 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     5161 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     5221 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     5281 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     5341 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     5401 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     5461 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5521 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     5581 accccgtaat tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg
     5641 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
     5701 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
     5761 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
     5821 gagtgcacca taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg
     5881 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5941 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6001 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6061 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6121 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6181 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6241 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6301 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6361 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6421 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6481 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6541 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6601 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6661 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6721 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6781 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6841 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7741 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7801 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7861 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7921 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7981 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct
     8041 agaggtcgac ggtatacaga catgataaga tacattgatg agtttggaca aaccacaact
     8101 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     8161 accattataa gctgcaataa acaagttggg gtgggcgaag aactccagca tgagatcccc
     8221 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     8281 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     8341 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     8401 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     8461 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     8521 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     8581 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     8641 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     8701 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     8761 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     8821 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     8881 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     8941 gtggccag
//
