LOCUS       sequence-417952-        7405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of TAL1-long.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: MIGR1_FLAG_TAL1-long_dt tomato
FEATURES             Location/Qualifiers
     source          1..7405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             150..173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1171..1738
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1188..1740
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1332..1349)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1559..1578
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1752..2453
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(1885..1903)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2153..2172
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2347..2370
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             2523..3037
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3199..3215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3223..3229,3230..3247,3248..3253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3268..3289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3406..3423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3577..4165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3657..3676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4336..5127,5128..5196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4959..4978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5197..5301)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5369..5387
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5425..5447)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5547..5566
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5760..5782
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5910..5929
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6129..6645
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6709..7050
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(7117..7405,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatct gccaccatgg attacaagga tgacgacgat aagaccgagc
      181 ggccgccgag cgaggcggct cgcagtgacc cccagctaga gggacgggac gcggccgagg
      241 ccagcatggc ccccccgcac ctggtcctgc tgaacggcgt cgccaaggag acgagccgcg
      301 cggccgcagc ggagccccca gtcatcgaac tgggcgcgcg cggaggcccg gggggcggcc
      361 ctgccggtgg gggcggcgcc gcgagagact taaagggccg cgacgcggcg acggccgaag
      421 cgcgccatcg ggtgcccacc accgagctgt gcagacctcc cgggcccgcc ccggcccccg
      481 cgcccgcctc ggttacagcg gagctgcccg gcgacggccg catggtgcag ctgagtcctc
      541 ccgcgctggc tgcccccgcc gcccccggcc gcgcgctgct ctacagcctc agccagccgc
      601 tggcctctct cggcagcggg ttctttgggg agccggatgc cttccctatg ttcaccacca
      661 acaatcgagt gaagaggaga ccttccccct atgagatgga gattactgat ggtccccaca
      721 ccaaagttgt gcggcgtatc ttcaccaaca gccgggagcg atggcggcag cagaatgtga
      781 acggggcctt tgccgagctc cgcaagctga tccccacaca tcccccggac aagaagctca
      841 gcaagaatga gatcctccgc ctggccatga agtatatcaa cttcttggcc aagctgctca
      901 atgaccagga ggaggagggc acccagcggg ccaagactgg caaggaccct gtggtggggg
      961 ctggtggggg tggaggtggg ggagggggcg gcgcgccccc agatgacctc ctgcaagacg
     1021 tgctttcccc caactccagc tgcggcagct ccctggatgg ggcagccagc ccggacagct
     1081 acacggagga gcccgcgccc aagcacacgg cccgcagcct ccatcctgcc atgctgcctg
     1141 ccgccgatgg agccggccct cggtgagaat tccgcccccc ccccctaacg ttactggccg
     1201 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     1261 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     1321 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     1381 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     1441 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     1501 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     1561 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     1621 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     1681 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     1741 tggccacaac catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
     1801 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1861 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     1921 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     1981 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     2041 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     2101 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     2161 tgcagaagaa gacgatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     2221 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     2281 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     2341 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     2401 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtgagtcg
     2461 acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     2521 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     2581 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     2641 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     2701 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     2761 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     2821 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     2881 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca
     2941 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     3001 ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg
     3061 agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg
     3121 ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc gcagaaagag
     3181 ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4621 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5341 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     5401 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5461 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5521 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5581 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     5641 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5701 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5761 ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc
     5821 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     5881 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     5941 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa
     6001 tttgttaaag acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag
     6061 ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa
     6121 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     6181 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
     6241 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     6301 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     6361 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     6421 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     6481 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
     6541 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
     6601 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
     6661 gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc
     6721 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
     6781 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     6841 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     6901 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     6961 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     7021 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     7081 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     7141 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     7201 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     7261 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     7321 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     7381 gcttttgacc cccctccctg ggtca
//
