LOCUS       sequence-417953-        6387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV viral vector packaging plasmid that expresses ""improved Cre 
            recombinase"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6387)
  TITLE     Harvey/Richie Labs  AAV Cre plasmids
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOttc556 - pAAV EF1a iCre
FEATURES             Location/Qualifiers
     source          1..6387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   21..150
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        251..1429
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          482..1420
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1377..1397
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1452..1472
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2534..3122
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2587..2607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3005..3016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3154..3630
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3353..3372)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3670..3810
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3670..3799
                     /label=AAV2 ITR
     rep_origin      3885..4340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3972..3991)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4182..4203
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4357..4376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4476..4498
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4536..4554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4622..4726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4727..4795,4796..5587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4945..4964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5758..6346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6247..6266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcctttt gctcacatgt cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc
       61 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      121 gagtggccaa ctccatcact aggggttcct gcggccgcac gcgtaagctt tgcaaagatg
      181 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
      241 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      301 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
      361 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      421 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      481 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
      541 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      601 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      661 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      721 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      781 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      841 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
      901 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
      961 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     1021 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     1081 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     1141 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1201 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1261 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1321 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1381 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc
     1441 ccaccatggt gcccaagaag aagaggaaag tctccaacct gctgactgtg caccaaaacc
     1501 tgcctgccct ccctgtggat gccacctctg atgaagtcag gaagaacctg atggacatgt
     1561 tcagggacag gcaggccttc tctgaacaca cctggaagat gctcctgtct gtgtgcagat
     1621 cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa cctgaggatg
     1681 tgagggacta cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc atccaacagc
     1741 acctgggcca gctcaacatg ctgcacagga gatctggcct gcctcgccct tctgactcca
     1801 atgctgtgtc cctggtgatg aggagaatca gaaaggagaa tgtggatgct ggggagagag
     1861 ccaagcaggc cctggccttt gaacgcactg actttgacca agtcagatcc ctgatggaga
     1921 actctgacag atgccaggac atcaggaacc tggccttcct gggcattgcc tacaacaccc
     1981 tgctgcgcat tgccgaaatt gccagaatca gagtgaagga catctcccgc accgatggtg
     2041 ggagaatgct gatccacatt ggcaggacca agaccctggt gtccacagct ggtgtggaga
     2101 aggccctgtc cctgggggtt accaagctgg tggagagatg gatctctgtg tctggtgtgg
     2161 ctgatgaccc caacaactac ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt
     2221 ctgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc acccaccgcc
     2281 tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct ggccactctg
     2341 ccagagtggg tgctgccagg gacatggcca gggctggtgt gtccatccct gaaatcatgc
     2401 aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac ctggactctg
     2461 agactggggc catggtgagg ctgctcgagg atggggactg aggcgcgccg aattcgatat
     2521 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2581 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2641 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2701 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2761 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2821 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2881 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2941 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3001 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3061 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3121 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
     3181 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3241 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3301 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3361 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     3421 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     3481 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     3541 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     3601 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg
     3661 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     3721 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     3781 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     3841 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     3901 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3961 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4021 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4081 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4141 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4201 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4261 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4321 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     4381 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4441 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4501 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4561 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6361 ggccttttta cggttcctgg ccttttg
//
