LOCUS       sequence-417956-        7455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of TAL1-long.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: MIGR1_FLAG_TAL1-long_GFP
FEATURES             Location/Qualifiers
     source          1..7455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             150..173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1171..1738
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1188..1740
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1332..1349)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1559..1578
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1752..1754,1755..1757,1758..2471)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1797..1818)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2058..2077)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2405..2426
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2573..3087
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3249..3265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3273..3279,3280..3297,3298..3303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3318..3339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3456..3473)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3627..4215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3707..3726)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4386..5177,5178..5246))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5009..5028
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5247..5351)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5419..5437
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5475..5497)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5597..5616
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5810..5832
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5960..5979
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6179..6695
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6759..7100
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(7167..7455,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatct gccaccatgg attacaagga tgacgacgat aagaccgagc
      181 ggccgccgag cgaggcggct cgcagtgacc cccagctaga gggacgggac gcggccgagg
      241 ccagcatggc ccccccgcac ctggtcctgc tgaacggcgt cgccaaggag acgagccgcg
      301 cggccgcagc ggagccccca gtcatcgaac tgggcgcgcg cggaggcccg gggggcggcc
      361 ctgccggtgg gggcggcgcc gcgagagact taaagggccg cgacgcggcg acggccgaag
      421 cgcgccatcg ggtgcccacc accgagctgt gcagacctcc cgggcccgcc ccggcccccg
      481 cgcccgcctc ggttacagcg gagctgcccg gcgacggccg catggtgcag ctgagtcctc
      541 ccgcgctggc tgcccccgcc gcccccggcc gcgcgctgct ctacagcctc agccagccgc
      601 tggcctctct cggcagcggg ttctttgggg agccggatgc cttccctatg ttcaccacca
      661 acaatcgagt gaagaggaga ccttccccct atgagatgga gattactgat ggtccccaca
      721 ccaaagttgt gcggcgtatc ttcaccaaca gccgggagcg atggcggcag cagaatgtga
      781 acggggcctt tgccgagctc cgcaagctga tccccacaca tcccccggac aagaagctca
      841 gcaagaatga gatcctccgc ctggccatga agtatatcaa cttcttggcc aagctgctca
      901 atgaccagga ggaggagggc acccagcggg ccaagactgg caaggaccct gtggtggggg
      961 ctggtggggg tggaggtggg ggagggggcg gcgcgccccc agatgacctc ctgcaagacg
     1021 tgctttcccc caactccagc tgcggcagct ccctggatgg ggcagccagc ccggacagct
     1081 acacggagga gcccgcgccc aagcacacgg cccgcagcct ccatcctgcc atgctgcctg
     1141 ccgccgatgg agccggccct cggtgagaat tccgcccccc ccccctaacg ttactggccg
     1201 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     1261 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     1321 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     1381 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     1441 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     1501 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     1561 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     1621 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     1681 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     1741 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1801 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg
     1861 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1921 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1981 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2041 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2101 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2161 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     2221 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcagcg
     2281 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2341 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2401 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2461 tgtacaagta atgaattaat taagaattat caagcttatc gataccgtcg acctgcagcc
     2521 aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     2581 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
     2641 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
     2701 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     2761 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     2821 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     2881 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     2941 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc
     3001 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     3061 gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat
     3121 tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc
     3181 acatactcca atactcctga aatagttcat tatggacagc gcagaaagag ctggggagaa
     3241 ttgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3301 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3361 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3421 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3481 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3541 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3601 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3781 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4201 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4261 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4321 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4381 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4441 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4501 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4561 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4621 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4681 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4741 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4801 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4861 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4921 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4981 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5041 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5101 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5161 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5221 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5281 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5341 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5401 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     5461 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5521 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5581 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     5641 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     5701 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     5761 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     5821 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     5881 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     5941 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     6001 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag
     6061 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     6121 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     6181 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     6241 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
     6301 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     6361 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
     6421 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     6481 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     6541 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
     6601 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
     6661 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
     6721 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
     6781 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
     6841 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
     6901 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     6961 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     7021 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     7081 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     7141 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     7201 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     7261 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     7321 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     7381 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     7441 cccctccctg ggtca
//
