LOCUS       sequence-417958-        6969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of TAL1-short.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: MIGR1_FLAG_TAL1-short_GFP
FEATURES             Location/Qualifiers
     source          1..6969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(6..797,798..866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     629..648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(867..971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1039..1057
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1095..1117)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1217..1236
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1430..1452
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1580..1599
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             1799..2315
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2379..2720
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2787..3203
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3133..3155
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3171..3187
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      3211..3220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3220..3243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3777..4329
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3921..3938)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4148..4167
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4341..4343,4344..4346,4347..5060)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4386..4407)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4647..4666)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4994..5015
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5162..5676
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5838..5854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5862..5868,5869..5886,5887..5892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5907..5928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6045..6062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6216..6804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6296..6315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
       61 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
      181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
      241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
      301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
      361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
      421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
      481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
      541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
      601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
      661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
      721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
      781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
      841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
      901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
      961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     1021 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     1081 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     1141 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     1201 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     1261 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     1321 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     1381 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     1441 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     1501 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     1561 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     1621 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag
     1681 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     1741 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     1801 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     1861 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
     1921 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     1981 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
     2041 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     2101 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     2161 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
     2221 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
     2281 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
     2341 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
     2401 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
     2461 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
     2521 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     2581 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     2641 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     2701 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     2761 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     2821 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     2881 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     2941 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     3001 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     3061 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     3121 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     3181 ccctcactcc ttctctaggc gccggaatta gccaccatgg attacaagga tgacgacgat
     3241 aagttcacca ccaacaatcg agtgaagagg agaccttccc cctatgagat ggagattact
     3301 gatggtcccc acaccaaagt tgtgcggcgt atcttcacca acagccggga gcgatggcgg
     3361 cagcagaatg tgaacggggc ctttgccgag ctccgcaagc tgatccccac acatcccccg
     3421 gacaagaagc tcagcaagaa tgagatcctc cgcctggcca tgaagtatat caacttcttg
     3481 gccaagctgc tcaatgacca ggaggaggag ggcacccagc gggccaagac tggcaaggac
     3541 cctgtggtgg gggctggtgg gggtggaggt gggggagggg gcggcgcgcc cccagatgac
     3601 ctcctgcaag acgtgctttc ccccaactcc agctgcggca gctccctgga tggggcagcc
     3661 agcccggaca gctacacgga ggagcccgcg cccaagcaca cggcccgcag cctccatcct
     3721 gccatgctgc ctgccgccga tggagccggc cctcggtgac cgcccccccc cccctaacgt
     3781 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3841 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3901 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3961 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4021 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4081 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4141 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4201 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4261 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4321 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4381 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg
     4441 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4501 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4561 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4621 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4681 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4741 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4801 tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
     4861 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4921 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     4981 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5041 tggacgagct gtacaagtaa tgaattaatt aagaattatc aagcttatcg ataccgtcga
     5101 cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga aaaagggggg
     5161 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     5221 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
     5281 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5341 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
     5401 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     5461 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     5521 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa
     5581 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     5641 tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga
     5701 gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt
     5761 tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg cagaaagagc
     5821 tggggagaat tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6961 acttggtct
//
