LOCUS       sequence-417960-        5874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 TSS5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_TSS5
FEATURES             Location/Qualifiers
     source          1..5874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1509..1540
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1567..1576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1732..3384
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3428..3549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3439..3458)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3493..3512
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3797..3814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3968..4556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4048..4067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4756..5547,5548..5616))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5721..5769
                     /note="synthetic polyadenylation signal"
     misc_feature    5783..5874
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5823..5842
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tgagcaggat agtggcatga ccagctctat cttctttttt
       61 tttttttgag atggagtctc actctgttgc ccaggctaga gtgcagtggc acgatctcgg
      121 ctcaccacaa cctccacctc ctgggttcaa gtgattcttc tgcctcagct tcctgagtag
      181 ctgggattaa aggcgtgcgc caccacaccc ggctcatttt gtatttttag tagagatggg
      241 gtttctccat gttggtcagg ctggtctcga ctcccgacct caggtgatcc acctgcctcg
      301 gtctcccaaa gtgctgggat tacaggcgtg agccaccgtg cccggcctga ccagctctat
      361 ctttaaaaga tcactccact gtgatgtgga gaaggggttg cagggcaatg gtggacacag
      421 gaagggctga taagaagcaa ctgtgatagt ccagacacct gcctctggga gcctaaaaca
      481 ctccacatag gtgggaaggc ttcaaaagag gcctggggat gaagctgttg ccctccagcc
      541 tgccgcgctt cccggggacc tgcaaacgtc catcctttcc tcggtcgatt cctcgtccag
      601 cctgtggtcc ttggggctct gatttattca gcttgggaac caggcagcga gtctcatgtc
      661 tgccccaggc ctcaaaatag attatttttt ttattagctg tcccttgtta actcataatt
      721 gcaatcatgt aacatttaat agaggcacaa agcgatcccc atcactgctc cacaatcatt
      781 cattagctaa caagacagag cagctcataa aaaaaaagcc gttaaaaaaa ttccggggaa
      841 atggaaagca ggaggtgatg caagccctgg ttaacaaagg ctgagggttg gggggaggca
      901 tgagagggtg tgagtggaat aacccaagcc tgataagcca caaagcagcg cctgctgctc
      961 cctcctccct ctgccgcctg agtcagagaa gccgggatgt gttcaaaatc aagcaatgta
     1021 attagcaggt tgcatcatgc ctctcgatta taaattacaa tggccacaaa gagggtggat
     1081 ggaggagcag ggattagatg gggtaaccca atcccagtgc cgggaagggg gacagagggc
     1141 ccgggagctg ccccccgcca ctaggagcca ctcggagtgg cctctgtcta ctgtgtctag
     1201 ggacagcaac aaggcctact caatacctgc tttgtgtagc ctgggataat gatttacaaa
     1261 ctcaaggaaa tcaggaggtg tgaaaagaag atagccctct taacccagcc attgtctacc
     1321 ctctgctgga atgtccctca gctccaccca cctggtgcac ctgtgcacac ggatgctgtt
     1381 tgaggatggt ccacgtgagg agccctcata ggggctggac tgataagatc taaaagggtt
     1441 gctgggctcg aggagctcgc tagcctcgag gatatcaaga tctggcctcg gcggccaagc
     1501 ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     1561 ggtaaagcca ccatggacag cggagctgcc cggcgacggc cgcatggtgc agctgagtcc
     1621 tcccgcgctg gctgcccccg ccgcccccgg ccgcgcgctg ctctacagcc tcagccagcc
     1681 gctggcctct ctcggcagcg ggttctttgg ggagccggat gccttccctc catggaagat
     1741 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
     1801 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
     1861 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
     1921 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
     1981 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
     2041 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
     2101 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
     2161 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
     2221 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
     2281 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
     2341 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
     2401 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt
     2461 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg
     2521 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa
     2581 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag
     2641 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta
     2701 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca
     2761 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc
     2821 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt
     2881 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac
     2941 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc
     3001 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa
     3061 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac
     3121 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca
     3181 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc
     3241 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct
     3301 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag
     3361 aagggcggca agatcgccgt gtaataattc tagagtcggg gcggccggcc gcttcgagca
     3421 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3481 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3541 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3601 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg
     3661 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     3721 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     3781 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3961 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct
     4741 aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct
     4801 atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg
     4861 cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga
     4921 tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt
     4981 gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt
     5041 gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt
     5101 cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat
     5161 attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc
     5221 cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc
     5281 cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat
     5341 acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag
     5401 tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt
     5461 gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc
     5521 ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa
     5581 gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg
     5641 aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata ttaaggtacg
     5701 ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     5761 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
     5821 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctct
//
