LOCUS       sequence-417964-        5878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 TSS4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_TSS4
FEATURES             Location/Qualifiers
     source          1..5878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1509..1540
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1567..1576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1736..3388
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3432..3553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3443..3462)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3497..3516
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3801..3818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3972..4560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4052..4071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4760..5551,5552..5620))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5725..5773
                     /note="synthetic polyadenylation signal"
     misc_feature    5787..5878
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5827..5846
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tgagcaggat agtggcatga ccagctctat cttctttttt
       61 tttttttgag atggagtctc actctgttgc ccaggctaga gtgcagtggc acgatctcgg
      121 ctcaccacaa cctccacctc ctgggttcaa gtgattcttc tgcctcagct tcctgagtag
      181 ctgggattaa aggcgtgcgc caccacaccc ggctcatttt gtatttttag tagagatggg
      241 gtttctccat gttggtcagg ctggtctcga ctcccgacct caggtgatcc acctgcctcg
      301 gtctcccaaa gtgctgggat tacaggcgtg agccaccgtg cccggcctga ccagctctat
      361 ctttaaaaga tcactccact gtgatgtgga gaaggggttg cagggcaatg gtggacacag
      421 gaagggctga taagaagcaa ctgtgatagt ccagacacct gcctctggga gcctaaaaca
      481 ctccacatag gtgggaaggc ttcaaaagag gcctggggat gaagctgttg ccctccagcc
      541 tgccgcgctt cccggggacc tgcaaacgtc catcctttcc tcggtcgatt cctcgtccag
      601 cctgtggtcc ttggggctct gatttattca gcttgggaac caggcagcga gtctcatgtc
      661 tgccccaggc ctcaaaatag attatttttt ttattagctg tcccttgtta actcataatt
      721 gcaatcatgt aacatttaat agaggcacaa agcgatcccc atcactgctc cacaatcatt
      781 cattagctaa caagacagag cagctcataa aaaaaaagcc gttaaaaaaa ttccggggaa
      841 atggaaagca ggaggtgatg caagccctgg ttaacaaagg ctgagggttg gggggaggca
      901 tgagagggtg tgagtggaat aacccaagcc tgataagcca caaagcagcg cctgctgctc
      961 cctcctccct ctgccgcctg agtcagagaa gccgggatgt gttcaaaatc aagcaatgta
     1021 attagcaggt tgcatcatgc ctctcgatta taaattacaa tggccacaaa gagggtggat
     1081 ggaggagcag ggattagatg gggtaaccca atcccagtgc cgggaagggg gacagagggc
     1141 ccgggagctg ccccccgcca ctaggagcca ctcggagtgg cctctgtcta ctgtgtctag
     1201 ggacagcaac aaggcctact caatacctgc tttgtgtagc ctgggataat gatttacaaa
     1261 ctcaaggaaa tcaggaggtg tgaaaagaag atagccctct taacccagcc attgtctacc
     1321 ctctgctgga atgtccctca gctccaccca cctggtgcac ctgtgcacac ggatgctgtt
     1381 tgaggatggt ccacgtgagg agccctcata ggggctggac tgataagatc taaaagggtt
     1441 gctgggctcg aggagctcgc tagcctcgag gatatcaaga tctggcctcg gcggccaagc
     1501 ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     1561 ggtaaagcca ccatggatcg cccaggacca caccgcagcg taactgcagg cctctcagcg
     1621 aaaaaggggg aaagcaaaga cccgggtgtg catcctcttc ctcggcttcc gcccctttcc
     1681 ggcggagtgg agatcctatt cagaggggcc ggtctctcta aatatgcccc agcccatgga
     1741 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
     1801 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
     1861 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
     1921 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
     1981 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
     2041 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
     2101 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
     2161 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     2221 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     2281 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     2341 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     2401 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     2461 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     2521 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     2581 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     2641 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     2701 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     2761 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     2821 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     2881 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     2941 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     3001 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     3061 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     3121 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     3181 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     3241 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     3301 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     3361 caagaagggc ggcaagatcg ccgtgtaata attctagagt cggggcggcc ggccgcttcg
     3421 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     3481 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     3541 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     3601 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     3661 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     3721 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     3781 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3961 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4381 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4441 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4501 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4561 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4621 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4681 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa
     4741 tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct
     4801 gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg
     4861 agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc
     4921 ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta
     4981 ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc
     5041 cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt
     5101 cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac
     5161 ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt
     5221 tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc
     5281 catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt
     5341 gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata
     5401 gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga
     5461 tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag
     5521 catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa
     5581 agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt
     5641 attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg
     5701 tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
     5761 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     5821 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctct
//
