LOCUS       sequence-417965-        5090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL3 promoter luciferase reporter vector containing 3 copies of the 
            NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) 
            used for luciferase reporter assays in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Munoz-Tello P, Lin H, Khan P, de Vera IMS, Kamenecka TM, Kojetin DJ
  TITLE     Assessment of NR4A Ligands That Directly Bind and Modulate the 
            Orphan Nuclear Receptor Nurr1.
  JOURNAL   J Med Chem. 2020 Dec 24;63(24):15639-15654. doi: 
            10.1021/acs.jmedchem.0c00894. Epub 2020 Dec 8.
  PUBMED    33289551
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.0c00894"; journalName: "J Med Chem"; date: 
            "2020-12-24- 24"; volume: "63"; issue: "24"; pages: "15639-15654"
COMMENT     Sequence Label: pGL3-NBRE3
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        128..324
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      175..310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             360..2012
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(426..444)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2053..2174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2064..2083)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2118..2137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2422..2439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2593..3181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2673..2692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3352..4143,4144..4212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3975..3994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4213..4317)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4344..4799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4431..4450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4641..4662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4930..4978
                     /note="synthetic polyadenylation signal"
     misc_feature    4992..5083
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5032..5051
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctaggagtt ttaaaaggtc atgctcaatt tgtcgagttt
       61 taaaaggtca tgctcaattt gtcgagtttt aaaaggtcat gctcaatttg tctcgagatc
      121 tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      181 cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt
      241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      301 tttggaggcc taggcttttg caaaaagctt ggcattccgg tactgttggt aaagccacca
      361 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
      421 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      481 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
      541 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      601 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
      661 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
      721 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
      781 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
      841 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
      901 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
      961 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     1021 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     1081 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     1141 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     1201 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     1261 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     1321 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     1381 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     1441 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     1501 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     1561 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     1621 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     1681 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     1741 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     1801 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     1861 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     1921 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     1981 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc
     2041 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2101 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2161 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2221 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2281 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2341 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2401 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3001 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4321 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4381 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4441 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4501 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4561 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4621 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4681 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4741 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
     4801 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4861 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg
     4921 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     4981 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     5041 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
//
