LOCUS       sequence-417967-       10141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, 
            Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary 
            JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ
  TITLE     The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 
            from chromatin.
  JOURNAL   Nat Cell Biol. 2022 Jan;24(1):62-73. doi: 
            10.1038/s41556-021-00807-6. Epub 2022 Jan 10.
  PUBMED    35013556
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-021-00807-6"; journalName: "Nat Cell Biol"; date: 
            "2022-01"; volume: "24"; issue: "1"; pages: "62-73"
COMMENT     Sequence Label: PB-PARP1–eGFP
FEATURES             Location/Qualifiers
     source          1..10141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   62..124
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     ncRNA           complement(486..500)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     524..540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        567..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        871..1074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1025..1045
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1103..1112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1112..1135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4226..4235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4232..4234,4235..4237,4238..4951)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4232..4234,4235..4237,4238..4948)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4277..4298)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4538..4557)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4885..4906
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4978..5551,5552..5554,5555..5563)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5144..5161)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5371..5390
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5645..6448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5708..5727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6318..6337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6571..6692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6608..6627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6662..6681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7131..7165
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7311..7329)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7350..7366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7350..7366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7363..7385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7374..7390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7398..7404,7405..7422,7423..7428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7443..7464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7581..7598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7752..8340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7832..7851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8511..9302,9303..9371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9134..9153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9372..9476)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9502..9957)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9639..9660)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9851..9870
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10099..10115
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10122..10141
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10122..10140
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgaattggcg gtattcacga cagcaggctg aataataaaa aaattagaaa ctattattta
       61 accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg
      121 catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt
      181 ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat
      241 ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttaa
      301 acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc atgttgtatt
      361 ataaaataag taattagctt aacctataca taatagaaac aaattatact tattagtcag
      421 tcagaaacaa ctttggccta aagtagcccc ttgaattcaa caaaagaaaa aaacgataaa
      481 agatacatct gtggcttcac tagctagccc cagtcacgac gttgtaaaac gacggccagt
      541 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
      601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      841 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      901 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      961 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1021 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     1081 gtgaaccgtc agatccgcta gcgccaccat ggactacaaa gacgatgacg acaagcttgc
     1141 cgccgcgaat tcaatggcgg agtcttcgga taagctctat cgagtcgagt acgccaagag
     1201 cgggcgcgcc tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc
     1261 catcatggtg cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg
     1321 cttctggaag gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga
     1381 gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg
     1441 caaaggccag gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga
     1501 gtatgccaag tccaacagaa gtacgtgcaa ggggtgtatg gagaagatag aaaagggcca
     1561 ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca cagctaggca tgattgaccg
     1621 ctggtaccat ccaggctgct ttgtcaagaa cagggaggag ctgggtttcc ggcccgagta
     1681 cagtgcgagt cagctcaagg gcttcagcct ccttgctaca gaggataaag aagccctgaa
     1741 gaagcagctc ccaggagtca agagtgaagg aaagagaaaa ggcgatgagg tggatggagt
     1801 ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac aaggatagta agcttgaaaa
     1861 agccctaaag gctcagaacg acctgatctg gaacatcaag gacgagctaa agaaagtgtg
     1921 ttcaactaat gacctgaagg agctactcat cttcaacaag cagcaagtgc cttctgggga
     1981 gtcggcgatc ttggaccgag tagctgatgg catggtgttc ggtgccctcc ttccctgcga
     2041 ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat tactgcactg gggacgtcac
     2101 tgcctggacc aagtgtatgg tcaagacaca gacacccaac cggaaggagt gggtaacccc
     2161 aaaggaattc cgagaaatct cttacctcaa gaaattgaag gttaaaaagc aggaccgtat
     2221 attcccccca gaaaccagcg cctccgtggc ggccacgcct ccgccctcca cagcctcggc
     2281 tcctgctgct gtgaactcct ctgcttcagc agataagcca ttatccaaca tgaagatcct
     2341 gactctcggg aagctgtccc ggaacaagga tgaagtgaag gccatgattg agaaactcgg
     2401 ggggaagttg acggggacgg ccaacaaggc ttccctgtgc atcagcacca aaaaggaggt
     2461 ggaaaagatg aataagaaga tggaggaagt aaaggaagcc aacatccgag ttgtgtctga
     2521 ggacttcctc caggacgtct ccgcctccac caagagcctt caggagttgt tcttagcgca
     2581 catcttgtcc ccttgggggg cagaggtgaa ggcagagcct gttgaagttg tggccccaag
     2641 agggaagtca ggggctgcgc tctccaaaaa aagcaagggc caggtcaagg aggaaggtat
     2701 caacaaatct gaaaagagaa tgaaattaac tcttaaagga ggagcagctg tggatcctga
     2761 ttctggactg gaacactctg cgcatgtcct ggagaaaggt gggaaggtct tcagtgccac
     2821 ccttggcctg gtggacatcg ttaaaggaac caactcctac tacaagctgc agcttctgga
     2881 ggacgacaag gaaaacaggt attggatatt caggtcctgg ggccgtgtgg gtacggtgat
     2941 cggtagcaac aaactggaac agatgccgtc caaggaggat gccattgagc acttcatgaa
     3001 attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa aatttcacga agtatcccaa
     3061 aaagttctac cccctggaga ttgactatgg ccaggatgaa gaggcagtga agaagctgac
     3121 agtaaatcct ggcaccaagt ccaagctccc caagccagtt caggacctca tcaagatgat
     3181 ctttgatgtg gaaagtatga agaaagccat ggtggagtat gagatcgacc ttcagaagat
     3241 gcccttgggg aagctgagca aaaggcagat ccaggccgca tactccatcc tcagtgaggt
     3301 ccagcaggcg gtgtctcagg gcagcagcga ctctcagatc ctggatctct caaatcgctt
     3361 ttacaccctg atcccccacg actttgggat gaagaagcct ccgctcctga acaatgcaga
     3421 cagtgtgcag gccaaggtgg aaatgcttga caacctgctg gacatcgagg tggcctacag
     3481 tctgctcagg ggagggtctg atgatagcag caaggatccc atcgatgtca actatgagaa
     3541 gctcaaaact gacattaagg tggttgacag agattctgaa gaagccgaga tcatcaggaa
     3601 gtatgttaag aacactcatg caaccacaca caatgcgtat gacttggaag tcatcgatat
     3661 ctttaagata gagcgtgaag gcgaatgcca gcgttacaag ccctttaagc agcttcataa
     3721 ccgaagattg ctgtggcacg ggtccaggac caccaacttt gctgggatcc tgtcccaggg
     3781 tcttcggata gccccgcctg aagcgcccgt gacaggctac atgtttggta aagggatcta
     3841 tttcgctgac atggtctcca agagtgccaa ctactgccat acgtctcagg gagacccaat
     3901 aggcttaatc ctgttgggag aagttgccct tggaaacatg tatgaactga agcacgcttc
     3961 acatatcagc aagttaccca agggcaagca cagtgtcaaa ggtttgggca aaactacccc
     4021 tgatccttca gctaacatta gtctggatgg tgtagacgtt cctcttggga ccgggatttc
     4081 atctggtgtg aatgacacct ctctactata taacgagtac attgtctatg atattgctca
     4141 ggtaaatctg aagtatctgc tgaaactgaa attcaatttt aagacctccc tgtggtcggt
     4201 accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     4261 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4321 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4381 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4441 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4501 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4561 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4621 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4681 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4741 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4801 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4861 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4921 cactctcggc atggacgagc tgtacaagta accgcggtgg taagaattcc gccccccccc
     4981 ctctccctcc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     5041 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5101 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5161 cagggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5221 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     5281 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     5341 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     5401 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     5461 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     5521 gtggttttcc tttgaaaaac acgatgataa tatggccaca acctgttaca ttgcacaaga
     5581 taaaaatata tcatcacgaa cagtaaaact gtctgcttac ataaacagta atacaagggg
     5641 tgttatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5701 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5761 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5821 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5881 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5941 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6001 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6061 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6121 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6181 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6241 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6301 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6361 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6421 ctatcgcctt cttgacgagt tcttctgaag cggccgcgac tctagatcat aatcagccat
     6481 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     6541 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     6601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6661 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaaggg taccaggatc
     6721 cctaaagcgc atgctccaga ctgtttattt tttttttcat tttttttttg gtcgaacgag
     6781 gaggttcaag tgcagaagag cagagaggat atgctcatcg tctaaagaac tacccatttt
     6841 attatatatt agtcacgata tctataacaa gaaaatatat atataataag ttatcacgta
     6901 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     6961 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     7021 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     7081 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     7141 ttttacgcag actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt
     7201 taaacacgtc atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc
     7261 gacgcgcgct aaagaatggc aaaccaagtc gcgcgagcca gcttttgttc cctttagtga
     7321 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7381 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7441 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7501 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7561 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7621 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7681 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7741 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7801 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7861 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7921 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7981 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8041 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8101 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8161 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8221 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8281 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8341 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8401 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8461 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8521 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8581 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8641 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8701 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8761 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8821 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8881 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8941 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9001 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9061 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9121 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9181 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9241 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9301 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9361 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9421 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9481 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9541 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9601 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9661 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9721 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9781 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9841 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9901 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     9961 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10021 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10081 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
    10141 g
//
