LOCUS       sequence-417969-       10879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, 
            Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary 
            JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ
  TITLE     The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 
            from chromatin.
  JOURNAL   Nat Cell Biol. 2022 Jan;24(1):62-73. doi: 
            10.1038/s41556-021-00807-6. Epub 2022 Jan 10.
  PUBMED    35013556
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-021-00807-6"; journalName: "Nat Cell Biol"; date: 
            "2022-01"; volume: "24"; issue: "1"; pages: "62-73"
COMMENT     Sequence Label: PB-PARP1-Apex2–eGFP
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   62..124
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     ncRNA           complement(486..500)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     524..540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        567..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        871..1074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1025..1045
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1103..1112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1112..1135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4208..4954
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     regulatory      4964..4973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4970..4972,4973..4975,4976..5689)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4970..4972,4973..4975,4976..5686)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5015..5036)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5276..5295)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5623..5644
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5716..6289,6290..6292,6293..6301)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5882..5899)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6109..6128
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6383..7186
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6446..6465)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7056..7075
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7309..7430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7346..7365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7400..7419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7869..7903
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8049..8067)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8088..8104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8088..8104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8101..8123)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8112..8128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8136..8142,8143..8160,8161..8166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8181..8202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8319..8336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8490..9078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8570..8589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9249..10040,10041..10109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9872..9891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10110..10214)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10240..10695)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10377..10398)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10589..10608
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10837..10853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10860..10879
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10860..10878
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgaattggcg gtattcacga cagcaggctg aataataaaa aaattagaaa ctattattta
       61 accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg
      121 catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt
      181 ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat
      241 ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttaa
      301 acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc atgttgtatt
      361 ataaaataag taattagctt aacctataca taatagaaac aaattatact tattagtcag
      421 tcagaaacaa ctttggccta aagtagcccc ttgaattcaa caaaagaaaa aaacgataaa
      481 agatacatct gtggcttcac tagctagccc cagtcacgac gttgtaaaac gacggccagt
      541 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
      601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      841 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      901 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      961 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1021 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     1081 gtgaaccgtc agatccgcta gcgccaccat ggactacaaa gacgatgacg acaagcttgc
     1141 cgccgcgaat tcaatggcgg agtcttcgga taagctctat cgagtcgagt acgccaagag
     1201 cgggcgcgcc tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc
     1261 catcatggtg cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg
     1321 cttctggaag gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga
     1381 gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg
     1441 caaaggccag gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga
     1501 gtatgccaag tccaacagaa gtacgtgcaa ggggtgtatg gagaagatag aaaagggcca
     1561 ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca cagctaggca tgattgaccg
     1621 ctggtaccat ccaggctgct ttgtcaagaa cagggaggag ctgggtttcc ggcccgagta
     1681 cagtgcgagt cagctcaagg gcttcagcct ccttgctaca gaggataaag aagccctgaa
     1741 gaagcagctc ccaggagtca agagtgaagg aaagagaaaa ggcgatgagg tggatggagt
     1801 ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac aaggatagta agcttgaaaa
     1861 agccctaaag gctcagaacg acctgatctg gaacatcaag gacgagctaa agaaagtgtg
     1921 ttcaactaat gacctgaagg agctactcat cttcaacaag cagcaagtgc cttctgggga
     1981 gtcggcgatc ttggaccgag tagctgatgg catggtgttc ggtgccctcc ttccctgcga
     2041 ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat tactgcactg gggacgtcac
     2101 tgcctggacc aagtgtatgg tcaagacaca gacacccaac cggaaggagt gggtaacccc
     2161 aaaggaattc cgagaaatct cttacctcaa gaaattgaag gttaaaaagc aggaccgtat
     2221 attcccccca gaaaccagcg cctccgtggc ggccacgcct ccgccctcca cagcctcggc
     2281 tcctgctgct gtgaactcct ctgcttcagc agataagcca ttatccaaca tgaagatcct
     2341 gactctcggg aagctgtccc ggaacaagga tgaagtgaag gccatgattg agaaactcgg
     2401 ggggaagttg acggggacgg ccaacaaggc ttccctgtgc atcagcacca aaaaggaggt
     2461 ggaaaagatg aataagaaga tggaggaagt aaaggaagcc aacatccgag ttgtgtctga
     2521 ggacttcctc caggacgtct ccgcctccac caagagcctt caggagttgt tcttagcgca
     2581 catcttgtcc ccttgggggg cagaggtgaa ggcagagcct gttgaagttg tggccccaag
     2641 agggaagtca ggggctgcgc tctccaaaaa aagcaagggc caggtcaagg aggaaggtat
     2701 caacaaatct gaaaagagaa tgaaattaac tcttaaagga ggagcagctg tggatcctga
     2761 ttctggactg gaacactctg cgcatgtcct ggagaaaggt gggaaggtct tcagtgccac
     2821 ccttggcctg gtggacatcg ttaaaggaac caactcctac tacaagctgc agcttctgga
     2881 ggacgacaag gaaaacaggt attggatatt caggtcctgg ggccgtgtgg gtacggtgat
     2941 cggtagcaac aaactggaac agatgccgtc caaggaggat gccattgagc acttcatgaa
     3001 attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa aatttcacga agtatcccaa
     3061 aaagttctac cccctggaga ttgactatgg ccaggatgaa gaggcagtga agaagctgac
     3121 agtaaatcct ggcaccaagt ccaagctccc caagccagtt caggacctca tcaagatgat
     3181 ctttgatgtg gaaagtatga agaaagccat ggtggagtat gagatcgacc ttcagaagat
     3241 gcccttgggg aagctgagca aaaggcagat ccaggccgca tactccatcc tcagtgaggt
     3301 ccagcaggcg gtgtctcagg gcagcagcga ctctcagatc ctggatctct caaatcgctt
     3361 ttacaccctg atcccccacg actttgggat gaagaagcct ccgctcctga acaatgcaga
     3421 cagtgtgcag gccaaggtgg aaatgcttga caacctgctg gacatcgagg tggcctacag
     3481 tctgctcagg ggagggtctg atgatagcag caaggatccc atcgatgtca actatgagaa
     3541 gctcaaaact gacattaagg tggttgacag agattctgaa gaagccgaga tcatcaggaa
     3601 gtatgttaag aacactcatg caaccacaca caatgcgtat gacttggaag tcatcgatat
     3661 ctttaagata gagcgtgaag gcgaatgcca gcgttacaag ccctttaagc agcttcataa
     3721 ccgaagattg ctgtggcacg ggtccaggac caccaacttt gctgggatcc tgtcccaggg
     3781 tcttcggata gccccgcctg aagcgcccgt gacaggctac atgtttggta aagggatcta
     3841 tttcgctgac atggtctcca agagtgccaa ctactgccat acgtctcagg gagacccaat
     3901 aggcttaatc ctgttgggag aagttgccct tggaaacatg tatgaactga agcacgcttc
     3961 acatatcagc aagttaccca agggcaagca cagtgtcaaa ggtttgggca aaactacccc
     4021 tgatccttca gctaacatta gtctggatgg tgtagacgtt cctcttggga ccgggatttc
     4081 atctggtgtg aatgacacct ctctactata taacgagtac attgtctatg atattgctca
     4141 ggtaaatctg aagtatctgc tgaaactgaa attcaatttt aagacctccc tgtggtcggt
     4201 accgcgggga aagtcttacc caactgtgag tgctgattac caggacgccg ttgagaaggc
     4261 gaagaagaag ctcagaggct tcatcgctga gaagagatgc gctcctctaa tgctccgttt
     4321 ggcattccac tctgctggaa cctttgacaa gggcacgaag accggtggac ccttcggaac
     4381 catcaagcac cctgccgaac tggctcacag cgctaacaac ggtcttgaca tcgctgttag
     4441 gcttttggag ccactcaagg cggagttccc tattttgagc tacgccgatt tctaccagtt
     4501 ggctggcgtt gttgccgttg aggtcacggg tggacctaag gttccattcc accctggaag
     4561 agaggacaag cctgagccac caccagaggg tcgcttgccc gatcccacta agggttctga
     4621 ccatttgaga gatgtgtttg gcaaagctat ggggcttact gaccaagata tcgttgctct
     4681 atctgggggt cacactattg gagctgcaca caaggagcgt tctggatttg agggtccctg
     4741 gacctctaat cctcttattt tcgacaactc atacttcacg gagttgttga gtggtgagaa
     4801 ggaaggtctc cttcagctac cttctgacaa ggctcttttg tctgaccctg tattccgccc
     4861 tctcgttgac aaatatgcag cggacgaaga tgccttcttt gctgattacg ctgaggctca
     4921 ccaaaagctt tccgagcttg ggtttgctga tgccccaccg gtcgccacca tggtgagcaa
     4981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5161 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5401 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5461 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5581 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac cgcggtggta
     5701 agaattccgc ccccccccct ctccctcccc ccccctaacg ttactggccg aagccgcttg
     5761 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5821 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5881 cctctcgcca aaggaatgca gggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5941 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     6001 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     6061 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     6121 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     6181 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6241 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     6301 ctgttacatt gcacaagata aaaatatatc atcacgaaca gtaaaactgt ctgcttacat
     6361 aaacagtaat acaaggggtg ttatgggatc ggccattgaa caagatggat tgcacgcagg
     6421 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6481 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6541 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6601 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6661 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6721 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6781 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6841 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6901 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6961 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7021 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7081 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7141 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaagcg gccgcgactc
     7201 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     7261 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     7321 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7381 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     7441 tgtaagggta ccaggatccc taaagcgcat gctccagact gtttattttt tttttcattt
     7501 tttttttggt cgaacgagga ggttcaagtg cagaagagca gagaggatat gctcatcgtc
     7561 taaagaacta cccattttat tatatattag tcacgatatc tataacaaga aaatatatat
     7621 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     7681 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     7741 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     7801 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     7861 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaaaaa agatttgcgc
     7921 tttactcgac ctaaacttta aacacgtcat agaatcttcg tttgacaaaa accacattgt
     7981 ggccaagctg tgtgacgcga cgcgcgctaa agaatggcaa accaagtcgc gcgagccagc
     8041 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     8101 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8161 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8221 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8281 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8341 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8401 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8461 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8521 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8581 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8641 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8701 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8761 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8821 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8881 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8941 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9001 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9061 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9121 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9181 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9241 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9301 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9361 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9421 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9481 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9541 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9601 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9661 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9721 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9781 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9841 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9901 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9961 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10021 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10081 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10141 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10201 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
    10261 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
    10321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
    10381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    10441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
    10501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    10561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    10621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    10681 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10741 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10801 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
    10861 aatacgactc actataggg
//
