LOCUS       sequence-417970-        5914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 TSS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_TSS2
FEATURES             Location/Qualifiers
     source          1..5914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1509..1540
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1567..1576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1772..3424
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3468..3589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3479..3498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3533..3552
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3837..3854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4008..4596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4088..4107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4796..5587,5588..5656))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5761..5809
                     /note="synthetic polyadenylation signal"
     misc_feature    5823..5914
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5863..5882
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tgagcaggat agtggcatga ccagctctat cttctttttt
       61 tttttttgag atggagtctc actctgttgc ccaggctaga gtgcagtggc acgatctcgg
      121 ctcaccacaa cctccacctc ctgggttcaa gtgattcttc tgcctcagct tcctgagtag
      181 ctgggattaa aggcgtgcgc caccacaccc ggctcatttt gtatttttag tagagatggg
      241 gtttctccat gttggtcagg ctggtctcga ctcccgacct caggtgatcc acctgcctcg
      301 gtctcccaaa gtgctgggat tacaggcgtg agccaccgtg cccggcctga ccagctctat
      361 ctttaaaaga tcactccact gtgatgtgga gaaggggttg cagggcaatg gtggacacag
      421 gaagggctga taagaagcaa ctgtgatagt ccagacacct gcctctggga gcctaaaaca
      481 ctccacatag gtgggaaggc ttcaaaagag gcctggggat gaagctgttg ccctccagcc
      541 tgccgcgctt cccggggacc tgcaaacgtc catcctttcc tcggtcgatt cctcgtccag
      601 cctgtggtcc ttggggctct gatttattca gcttgggaac caggcagcga gtctcatgtc
      661 tgccccaggc ctcaaaatag attatttttt ttattagctg tcccttgtta actcataatt
      721 gcaatcatgt aacatttaat agaggcacaa agcgatcccc atcactgctc cacaatcatt
      781 cattagctaa caagacagag cagctcataa aaaaaaagcc gttaaaaaaa ttccggggaa
      841 atggaaagca ggaggtgatg caagccctgg ttaacaaagg ctgagggttg gggggaggca
      901 tgagagggtg tgagtggaat aacccaagcc tgataagcca caaagcagcg cctgctgctc
      961 cctcctccct ctgccgcctg agtcagagaa gccgggatgt gttcaaaatc aagcaatgta
     1021 attagcaggt tgcatcatgc ctctcgatta taaattacaa tggccacaaa gagggtggat
     1081 ggaggagcag ggattagatg gggtaaccca atcccagtgc cgggaagggg gacagagggc
     1141 ccgggagctg ccccccgcca ctaggagcca ctcggagtgg cctctgtcta ctgtgtctag
     1201 ggacagcaac aaggcctact caatacctgc tttgtgtagc ctgggataat gatttacaaa
     1261 ctcaaggaaa tcaggaggtg tgaaaagaag atagccctct taacccagcc attgtctacc
     1321 ctctgctgga atgtccctca gctccaccca cctggtgcac ctgtgcacac ggatgctgtt
     1381 tgaggatggt ccacgtgagg agccctcata ggggctggac tgataagatc taaaagggtt
     1441 gctgggctcg aggagctcgc tagcctcgag gatatcaaga tctggcctcg gcggccaagc
     1501 ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     1561 ggtaaagcca ccatggcctt acgcaatata cagaaatgcg cgaggctgtg cgatcgccca
     1621 ggaccacacc gcagcgtaac tgcaggcctc tcagcgaaaa agggggaaag caaagacccg
     1681 ggtgtgcatc ctcttcctcg gcttccgccc ctttccggcg gagtggagat cctattcaga
     1741 ggggccggtc tctctaaata tgccccagcc catggaagat gccaaaaaca ttaagaaggg
     1801 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
     1861 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     1921 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
     1981 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     2041 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     2101 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     2161 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     2221 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     2281 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     2341 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     2401 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     2461 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     2521 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     2581 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     2641 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     2701 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     2761 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     2821 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     2881 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     2941 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
     3001 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     3061 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     3121 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     3181 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     3241 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     3301 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     3361 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     3421 gtaataattc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga
     3481 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3541 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3601 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3661 aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct
     3721 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     3781 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg
     3841 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3901 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3961 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4021 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4081 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4141 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4201 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4261 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4321 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4381 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4441 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4501 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4561 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4621 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4681 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4741 atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc
     4801 agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg
     4861 cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg
     4921 cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc
     4981 cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta
     5041 tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg
     5101 tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact
     5161 ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca
     5221 gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg
     5281 taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga
     5341 ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt
     5401 gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca
     5461 tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt
     5521 cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt
     5581 cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa
     5641 aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat cagggttact
     5701 agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc
     5761 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     5821 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     5881 cccagtgcaa gtgcaggtgc cagaacattt ctct
//
