LOCUS       sequence-417984-        5932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 TSS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_TSS1
FEATURES             Location/Qualifiers
     source          1..5932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1509..1540
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1567..1576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1790..3442
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3486..3607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3497..3516)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3551..3570
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3855..3872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4026..4614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4106..4125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4814..5605,5606..5674))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5779..5827
                     /note="synthetic polyadenylation signal"
     misc_feature    5841..5932
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5881..5900
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tgagcaggat agtggcatga ccagctctat cttctttttt
       61 tttttttgag atggagtctc actctgttgc ccaggctaga gtgcagtggc acgatctcgg
      121 ctcaccacaa cctccacctc ctgggttcaa gtgattcttc tgcctcagct tcctgagtag
      181 ctgggattaa aggcgtgcgc caccacaccc ggctcatttt gtatttttag tagagatggg
      241 gtttctccat gttggtcagg ctggtctcga ctcccgacct caggtgatcc acctgcctcg
      301 gtctcccaaa gtgctgggat tacaggcgtg agccaccgtg cccggcctga ccagctctat
      361 ctttaaaaga tcactccact gtgatgtgga gaaggggttg cagggcaatg gtggacacag
      421 gaagggctga taagaagcaa ctgtgatagt ccagacacct gcctctggga gcctaaaaca
      481 ctccacatag gtgggaaggc ttcaaaagag gcctggggat gaagctgttg ccctccagcc
      541 tgccgcgctt cccggggacc tgcaaacgtc catcctttcc tcggtcgatt cctcgtccag
      601 cctgtggtcc ttggggctct gatttattca gcttgggaac caggcagcga gtctcatgtc
      661 tgccccaggc ctcaaaatag attatttttt ttattagctg tcccttgtta actcataatt
      721 gcaatcatgt aacatttaat agaggcacaa agcgatcccc atcactgctc cacaatcatt
      781 cattagctaa caagacagag cagctcataa aaaaaaagcc gttaaaaaaa ttccggggaa
      841 atggaaagca ggaggtgatg caagccctgg ttaacaaagg ctgagggttg gggggaggca
      901 tgagagggtg tgagtggaat aacccaagcc tgataagcca caaagcagcg cctgctgctc
      961 cctcctccct ctgccgcctg agtcagagaa gccgggatgt gttcaaaatc aagcaatgta
     1021 attagcaggt tgcatcatgc ctctcgatta taaattacaa tggccacaaa gagggtggat
     1081 ggaggagcag ggattagatg gggtaaccca atcccagtgc cgggaagggg gacagagggc
     1141 ccgggagctg ccccccgcca ctaggagcca ctcggagtgg cctctgtcta ctgtgtctag
     1201 ggacagcaac aaggcctact caatacctgc tttgtgtagc ctgggataat gatttacaaa
     1261 ctcaaggaaa tcaggaggtg tgaaaagaag atagccctct taacccagcc attgtctacc
     1321 ctctgctgga atgtccctca gctccaccca cctggtgcac ctgtgcacac ggatgctgtt
     1381 tgaggatggt ccacgtgagg agccctcata ggggctggac tgataagatc taaaagggtt
     1441 gctgggctcg aggagctcgc tagcctcgag gatatcaaga tctggcctcg gcggccaagc
     1501 ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     1561 ggtaaagcca ccatggcgct caggaccggg cctcaaaatg gccacacgcg tacccccgta
     1621 gcggaaaaac atcgcccagg accacaccgc agcgtaactg caggcctctc agcgaaaaag
     1681 ggggaaagca aagacccggg tgtgcatcct cttcctcggc ttccgcccct ttccggcgga
     1741 gtggagatcc tattcagagg ggccggtctc tctaaatatg ccccagccca tggaagatgc
     1801 caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga
     1861 gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga
     1921 cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc
     1981 agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa
     2041 tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc
     2101 agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac
     2161 cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc
     2221 gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat
     2281 gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga
     2341 gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt
     2401 gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga
     2461 ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca
     2521 ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct
     2581 catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc
     2641 tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta
     2701 cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg
     2761 tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga
     2821 aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa
     2881 ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt
     2941 gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa
     3001 ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc
     3061 ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata
     3121 caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat
     3181 cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt
     3241 cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag
     3301 ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa
     3361 aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa
     3421 gggcggcaag atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga
     3481 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3541 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3601 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     3661 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac
     3721 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     3781 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     3841 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3901 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3961 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4501 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4561 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4621 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4681 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4741 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa
     4801 accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat
     4861 ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct
     4921 taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt
     4981 tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt
     5041 ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga
     5101 gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg
     5161 gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat
     5221 tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg
     5281 cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg
     5341 taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac
     5401 ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta
     5461 ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc
     5521 cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt
     5581 ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg
     5641 gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa
     5701 gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg
     5761 aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     5821 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     5881 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//
