LOCUS       sequence-418007-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gRNA against PDLIM1 with puro and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Ishikawa M, Sugino S, Masuda Y, Tarumoto Y, Seto Y, Taniyama N, 
            Wagai F, Yamauchi Y, Kojima Y, Kiryu H, Yusa K, Eiraku M, Mochizuki 
            A
  TITLE     RENGE infers gene regulatory networks using time-series single-cell 
            RNA-seq data with CRISPR perturbations.
  JOURNAL   Commun Biol. 2023 Dec 28;6(1):1290. doi: 10.1038/s42003-023-05594-4.
  PUBMED    38155269
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-12-28"; volume: "6(1):1290. doi"; pages: " 
            10.1038/s42003-023-05594-4"
COMMENT     Sequence Label: pKLV2-U6gRNA5(PDLIM1_5-1)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    147..272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             319..499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(517..715)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(542..562)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(1030..1049)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1031..1049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1059..1076)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1059..1075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1068..1090)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1217..1672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1304..1323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1514..1535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1698..1802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1803..1871,1872..2663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2021..2040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2834..3422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3323..3342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3576..3593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3710..3731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3746..3751,3752..3769,3770..3776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3784..3800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3789..3811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3808..3824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3808..3824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3843..3863
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3845..3863
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    3889..4113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4102..4119
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             4571..4582
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4980..5000
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5060..5761)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(5762..5815)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5834..6430)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5914..5934)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6411..6430
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6470..6969)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6518..6537)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6911..6934
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(7098..7346)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7156..7175)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7326..7346)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7421..7538
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7836..7880)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8065..8298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact
       61 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
      121 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
      181 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
      241 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
      301 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
      361 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
      421 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
      481 ggtctaacca gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt
      541 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      601 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
      661 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
      721 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
      781 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
      841 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
      901 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
      961 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtacta
     1021 gtcaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     1081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1201 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     1261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     1321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     1441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     1561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     1621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     1681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     3721 agctcactca ttaggcaccc caggctttac actttatgct cccggctcgt atgttgtgtg
     3781 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     3841 gcgcaattaa ccctcactaa agggaacaaa agcttaatta acggccgccc atagagccca
     3901 ccgcatcccc agcatgcctg ctattgactt cccaatcctc ccccttgctg tcctgcccca
     3961 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
     4021 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     4081 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg gtttaaactg
     4141 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     4201 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     4261 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagactct
     4321 ggtctaacca gagagaccca gtacaggcaa aaagcagatc ttgacttctt tgggagtgaa
     4381 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     4441 aagtcattgg tcttaaaggt acctgcgggg aggcggccca aagggagatc cgactcgtct
     4501 gagggcgaag gcgtagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
     4561 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
     4621 tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat
     4681 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
     4741 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
     4801 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
     4861 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
     4921 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
     4981 atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat
     5041 ccagaggttg agcggccgct caattaagct tgtgccccag tttgctaggg aggtcgcagt
     5101 atctggccac tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc
     5161 tttccagtct gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct
     5221 tggatctata tgtggtcttg atgtttgcga tcagatggct cccgcccacg agcttcaggg
     5281 ccatgtcgtt tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct
     5341 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga
     5401 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
     5461 cgcccccgtc ctcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
     5521 tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc
     5581 tagtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
     5641 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt
     5701 tgtccacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca
     5761 ttgggccagg attctcctcc acgtcaccgc atgttaggag acttcctctg ccctctcctc
     5821 cggacccgcc gccggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca
     5881 cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg
     5941 cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca
     6001 cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga
     6061 accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg
     6121 ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg
     6181 cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca
     6241 ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct
     6301 cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg
     6361 cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt
     6421 actcggtcat ggtaagcttc agctgctcga gatctagatg gatgcaggtc gaaaggcccg
     6481 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
     6541 ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
     6601 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
     6661 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
     6721 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
     6781 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
     6841 ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
     6901 gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
     6961 cccctacccg gtagaattgg atccaaaaaa agcaccgact cggtgccact ttttcaagtt
     7021 gataacggac tagccttatt taaacttgct atgctgtttc cagcatagct cttaaacgga
     7081 gtcaaagact gctgcccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg
     7141 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
     7201 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
     7261 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat
     7321 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggacgcgta gggcccggta
     7381 ctaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
     7441 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     7501 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
     7561 gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg
     7621 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     7681 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     7741 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     7801 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     7861 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     7921 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     7981 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     8041 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     8101 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     8161 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     8221 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     8281 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     8341 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     8401 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgc ggccggccgc
     8461 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     8521 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     8581 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     8641 acagccttct
//
