LOCUS       sequence-418036-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production and purification of catalytically active SynCas.v10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Brogan DJ, Dalla Benetta E, Wang T, Lin CP, Chen F, Li H, Lin C, 
            Komives EA, Akbari OS
  TITLE     Synthetic type III-E CRISPR-Cas effectors for programmable 
            RNA-targeting
  JOURNAL   bioRxiv 2024.02.23.581838; doi: 
            https://doi.org/10.1101/2024.02.23.581838
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.23.581838; doi: https://doi.org/10.1101/2024.02.23.581838"
COMMENT     Sequence Label: T7-RBS-6xHis-SUMO-SynCas.v10 bacteria
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5..1087)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1049..1068
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1088..1165)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1371..1390
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1474..1493
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1474..1492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1493..1517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1532..1554
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1569..1613
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1620..1637
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1647..1664
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1668..1961
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGISIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             5793..5810
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5863..5881)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5877..5924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5961..6416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6048..6067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6258..6279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6509..7324)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6509..7324)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7232..7251
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7316..7335
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7446..8034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7935..7954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8188..8205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8220..8359
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8285..8304)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8445..8467
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8461..8652)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
       61 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
      121 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
      181 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
      241 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
      301 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
      361 gcatcgcagt gggaacgata ccctcattca gcatttgcat ggtttgttga aaaccggaca
      421 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
      481 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
      541 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
      601 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
      661 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
      721 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
      781 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
      841 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
      901 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
      961 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     1021 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     1081 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     1141 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
     1201 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     1261 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     1321 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     1381 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     1441 gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt
     1501 gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc
     1561 ccatgggcgg tctgaacgac atcttcgagg ctcagaaaat cgaatggcac gaaagcagcc
     1621 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg tcggactcag
     1681 aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca
     1741 atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt
     1801 taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat
     1861 tcttgtacga cggtattagc attcaagctg atcagacccc tgaagatttg gacatggagg
     1921 ataacgatat tattgaggct cacagagaac agattggtgg aagtatgacg actactatga
     1981 agatttcaat tgaattcctc gagccgtttc ggatgaccaa atggcaggaa agcactaggc
     2041 gaaacaagaa caacaaggaa tttgttcgcg gccaagcttt tgcaagatgg catagaaata
     2101 agaaggataa cacaaaaggg cggccttaca taactgggac attgctgcgg tccgcagtaa
     2161 ttagaagcgc ggaaaacctg ctgacattga gtgatgggaa aattagcgaa aagacatgtt
     2221 gtcctgggaa atttgatacg gaagataaag acaggttgct gcaattgagg cagcggtcca
     2281 cccttcgttg gaccgataag aacccctgtc cagataacgc tgaaacttac tgcccgtttt
     2341 gcgaacttct cgggagatcc ggtaacgatg gaaagaaggc tgagaagaag gattggcgct
     2401 ttcgtattca ttttggcaat ctttcccttc cagggaagcc cgatttcgac gggccaaaag
     2461 ctataggcag ccaacgggta cttaacaggg tcgatttcaa atccggtaag gcccatgact
     2521 tctttaaggc ttacgaagtc gaccatacta ggtttccccg cttcgaaggg gagattacca
     2581 tagataataa agtcagcgct gaagccagga aactgctctg tgattctctc aagtttacgg
     2641 atcggctgtg tggagctctg tgcgtaatca ggttcgatga atatacacca gcagccgata
     2701 gtgggaaaca aaccgaaaat gtccaagcag aaccgaatgc taatctcgct gaaaagaccg
     2761 cagagcaaat tattagcatt ctggacgata ataagaaaac cgaatatacc cgcctgcttg
     2821 ctgatgctat tcgttcactc cgccgctcta gtaaacttgt cgctggcttg ccaaaggatc
     2881 atgacgggaa ggatgaccat tacctctggg atatcggcaa gaagaagaaa gacgaaaact
     2941 ctgtcactat taggcaaatc cttacgacct cagcagacac caaggaactc aagaatgccg
     3001 gaaaatggag agaattctgc gagaagctgg gtgaagcgct gtacctcaag agtaaagaca
     3061 tgagtggcgg cctgaaaatt actcggagaa tactgggcga tgctgaattc catggaaagc
     3121 ccgatcggct tgaaaagagc cggtctgtgt caatcggatc tgtgttgaaa gaaactgtgg
     3181 tatgcggcga actggtcgct aaaacgccct tcttcttcgg agcgatagac gaagatgcaa
     3241 aacaaaccga cctgcaagta ctcctcactc ccgataacaa gtatagactg ccaagaagcg
     3301 ccgtgcgagg tatactccgt cgagatcttc aaacctattt tgatagccca tgcaatgctg
     3361 agttgggtgg acggccatgc atgtgtaaga cgtgtaggat tatgagaggg atcacggtga
     3421 tggatgcgcg cagtgagtac aatgccccgc cagaaataag gcatcgtacc cgcattaatc
     3481 ccttcacagg cacggttgcg gaaggtgccc tgtttaatat ggaagtagcc cccgagggga
     3541 ttgtctttcc atttcaactc cggtaccggg gctctgaaga tgggctgccc gatgcactga
     3601 aaacggtgtt gaaatggtgg gctgaggggc aggcattcat gagtggcgct gcctcaaccg
     3661 ggaagggccg attccggatg gaaaatgcta aatatgaaac gctggatctg agcgacgaga
     3721 atcaaaggaa tgactatctt aagaattggg gatggcgtga cgagaagggg ctcgaggaac
     3781 tgaagaaacg actgaactca ggtctgccag agcccggtaa ttatagggat ccaaaatgga
     3841 caaaagtagg gtatgagatt aaaatacagt cgccccttct tagcaacgat cctatcgcag
     3901 cattgttcga cactgcaaac cacgacgcgg tcgcgtataa gaaacgagtg tgggagaacg
     3961 gacgtatcat agagcgacac actttcaaag gagagtctat acgaggggtg ctgcgcactg
     4021 cacttgggcg tgagtacgga ttgttcgaat taaaacacga agactgtcct tgtagtcttt
     4081 gcacaatttt cggtaacgaa cacgaagcgg gcaaaatacg tttcgaagac ttagaaatcg
     4141 aggacggtgt tgccgaaaag cacttcgatc acgttgctat cgatcgcttc acaggcggag
     4201 ccgtagacaa gaagaaattc gacgatttct ctttagctgg ttccccggga aagcccatta
     4261 aactcaaagg gtgcttttgg ttgcgtgcgg atatatttag tgacgagaag aaggacgaga
     4321 agaagaaaat aataaacgct ctttgtgacg tgcgtgatgg cttgtaccct ctcggtggca
     4381 agggcggtgt cggatacggg tgggtaaaag agctgagaat aacggacggc ccagacgatt
     4441 ttaagctccc ggaaccgaaa tgtaaggaaa ttccgccaga cggtactcca agcgattacc
     4501 cttttccgca gttaaagcca ctgcaaacag agaaggataa agtttactcg ccccactact
     4561 ttctgaaacc agcagagaag aaagttgacc ggaactggga aaagcggctg atcggacacg
     4621 agcgtttcga taaggacctg ttgactggta aaattgagtg tacactgaaa acgctgagcc
     4681 ctttaataat accggacagt gaggtcgtta cggagaacgg aaacggccac aagtcttacc
     4741 agttcttcag attaaataat gaaatcatca ttccgggaag cgaaattcgg ggtatgatca
     4801 gttccgtgta cgaagcactt actaactctt gcttccgtgt cgtggacgac atcatcccgg
     4861 tggtgatctc ccgtagcgtg tcaccgtacc agatcgctca gaagctgccc agtccggatc
     4921 tgcgtccttg cgagcgtgag attcttgagg actcagaaat tggatcttta gacaagtgtt
     4981 cagagaaacg ccttttccgc ctgcatgaag acggtctttg cccatcgtgt cgtctgttcg
     5041 gcactacaca ttataaggga cgcgttcggt tcggcttcgc aaagcacgag ggcggcgaaa
     5101 agtggctcat ggacgacaag actaaggacg agtatctgac tttaccgttg ttggaacggc
     5161 cgcgcccaac atggagcatg ccaagcgacg aagacgacgt accgggacgg aaattctacg
     5221 tacaccacaa tggttgggag aaagttaagc aagatagttt agataataaa acgagcaaaa
     5281 ccgagaataa tcgaagcgtg gaaccactcg ataaggataa cctgtttaaa ttcgacgtgt
     5341 tcttcgagaa cctggaaccc tgggaacttg gtttgcttct ctactcatta gaattggaag
     5401 aggggttagc ccacaagttt ggaatggcaa aagcgttcgg cttcggctcg acaaagattg
     5461 acgccgacaa aatactgtta cgtgaggctc cgaacgagtg gcaagatgct accaatcgta
     5521 aggacgacat gttacgcaag ggcttcggcg agctgacatc ctggttcgac aaggattggg
     5581 acaaagtcga gcacatagac ggtctgcgtt ccttacttca gattccggaa gcgctttcaa
     5641 gagccgaagt gcgctacccg gaccttacgg aagacgaccc agagaagccc ccgggctacg
     5701 tgcaattgtc tgataagaaa gagggccgca agtacttgat ccgtccctgg caaccttggt
     5761 actaagtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
     5821 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     5881 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     5941 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6001 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6061 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6121 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6181 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6241 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6301 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6361 gctgatttaa caaaaattta acgcgaattt taacaaacta gtaacgttta caatttcagg
     6421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6481 aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact
     6541 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     6601 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     6661 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     6721 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     6781 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     6841 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     6901 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     6961 caacaatgtt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     7021 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     7081 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     7141 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     7201 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     7261 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc
     7321 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa
     7381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7501 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7561 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7621 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7681 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7741 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7801 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7861 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7921 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7981 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8041 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8101 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8161 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8221 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
     8281 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     8341 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8401 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8461 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     8521 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     8581 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     8641 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     8701 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     8761 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     8821 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     8881 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     8941 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     9001 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     9061 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
     9121 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     9181 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     9241 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     9301 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     9361 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     9421 tgcctaatga gtgagctaac ttacattaat tgcgtt
//
