LOCUS       sequence-418037-        9534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production and purification of catalytically inactive SynCas.v12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Brogan DJ, Dalla Benetta E, Wang T, Lin CP, Chen F, Li H, Lin C, 
            Komives EA, Akbari OS
  TITLE     Synthetic type III-E CRISPR-Cas effectors for programmable 
            RNA-targeting
  JOURNAL   bioRxiv 2024.02.23.581838; doi: 
            https://doi.org/10.1101/2024.02.23.581838
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.23.581838; doi: https://doi.org/10.1101/2024.02.23.581838"
COMMENT     Sequence Label: T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(31..1113)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1075..1094
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1114..1191)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1397..1416
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1500..1519
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1500..1518
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1519..1543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1558..1580
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1595..1639
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1646..1663
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1673..1690
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1694..1987
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGISIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             5897..5914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5967..5985)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5981..6028
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6065..6520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6152..6171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6362..6383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6613..7428)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6613..7428)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7336..7355
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7420..7439
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7550..8138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8039..8058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8292..8309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8324..8463
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8389..8408)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8549..8571
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8565..8756)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
       61 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      121 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
      181 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
      241 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
      301 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
      361 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgataccct cattcagcat
      421 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
      481 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
      541 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
      601 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
      661 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
      721 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
      781 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
      841 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
      901 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
      961 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     1021 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     1081 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     1141 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     1201 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     1261 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     1321 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     1381 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     1441 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     1501 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     1561 gtttaacttt aagaaggaga tataccccat gggcggtctg aacgacatct tcgaggctca
     1621 gaaaatcgaa tggcacgaaa gcagccatca tcatcatcat cacagcagcg gcctggtgcc
     1681 gcgcggcagc catatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc
     1741 agaagtcaag cctgagactc acatcaattt aaaggtgtcc gatggatctt cagagatctt
     1801 cttcaagatc aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca
     1861 gggtaaggaa atggactcct taagattctt gtacgacggt attagcattc aagctgatca
     1921 gacccctgaa gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat
     1981 tggtggaagt atgacgacta ctatgaagat ttcaattgaa ttcctcgagc cgtttcggat
     2041 gaccaaatgg caggaaagca ctaggcgaaa caagaacaac aaggaatttg ttcgcggcca
     2101 agcttttgca agatggcata gaaataagaa ggataacaca aaagggcggc cttacataac
     2161 tgggacattg ctgcggtccg cagtaattag aagcgcggaa aacctgctga cattgagtga
     2221 tgggaaaatt agcgaaaaga catgttgtcc tgggaaattt gatacggaag ataaagacag
     2281 gttgctgcaa ttgaggcagc ggtccaccct tcgttggacc gataagaacc cctgtccaga
     2341 taacgctgaa acttactgcc cgttttgcga acttctcggg agatccggta acgatggaaa
     2401 gaaggctgag aagaaggatt ggcgctttcg tattcatttt ggcaatcttt cccttccagg
     2461 gaagcccgat ttcgacgggc caaaagctat aggcagccaa cgggtactta acagggtcga
     2521 tttcaaatcc ggtaaggccc atgacttctt taaggcttac gaagtcgacc atactaggtt
     2581 tccccgcttc gaaggggaga ttaccataga taataaagtc agcgctgaag ccaggaaact
     2641 gctctgtgat tctctcaagt ttacggatcg gctgtgtgga gctctgtgcg taatcaggtt
     2701 cgatgaatat acaccagcag ccgatagtgg gaaacaaacc gaaaatgtcc aagcagaacc
     2761 gaatgctaat ctcgctgaaa agaccgcaga gcaaattatt agcattctgg acgataataa
     2821 gaaaaccgaa tatacccgcc tgcttgctga tgctattcgt tcactccgcc gctctagtaa
     2881 acttgtcgct ggcttgccaa aggatcatga cgggaaggat gaccattacc tctgggatat
     2941 cggcaagaag aagaaagacg aaaactctgt cactattagg caaatcctta cgacctcagc
     3001 agacaccaag gaactcaaga atgccggaaa atggagagaa ttctgcgaga agctgggtga
     3061 agcgctgtac ctcaagagta aagacatgag tggcggcctg aaaattactc ggagaatact
     3121 gggcgatgct gaattccatg gaaagcccga tcggcttgaa aagagccggt ctgtgtcaat
     3181 cggatctgtg ttgaaagaaa ctgtggtatg cggcgaactg gtcgctaaaa cgcccttctt
     3241 cttcggagcg atagacgaag atgcaaaaca aaccgcactg caagtactcc tcactcccga
     3301 taacaagtat agactgccaa gaagcgccgt gcgaggtata ctccgtcgag atcttcaaac
     3361 ctattttgat agcccatgca atgctgagtt gggtggacgg ccatgcatgt gtaagacgtg
     3421 taggattatg agagggatca cggtgatgga tgcgcgcagt gagtacaatg ccccgccaga
     3481 aataaggcat cgtacccgca ttaatccctt cacaggcacg gttgcggaag gtgccctgtt
     3541 taatatggaa gtagcccccg aggggattgt ctttccattt caactccggt accggggctc
     3601 tgaagatggg ctgcccgatg cactgaaaac ggtgttgaaa tggtgggctg aggggcaggc
     3661 attcatgagt ggcgctgcct caaccgggaa gggccgattc cggatggaaa atgctaaata
     3721 tgaaacgctg gatctgagcg acgagaatca aaggaatgac tatcttaaga attggggatg
     3781 gcgtgacgag aaggggctcg aggaactgaa gaaacgactg aactcaggtc tgccagagcc
     3841 cggtaattat agggatccaa aatggcacga gattaacgtt tccattgaga tggcaagccc
     3901 ttttattaat ggcgacccaa tccgcgcagc cgtggacaaa cgtggtacag cagtggttac
     3961 cttcgttaag tataaagctg aaggggaaga ggcgaaaccc gtatgtgcat acaaggccga
     4021 atcttttaga ggggtgatca gaagtgccgt ggcacgcatt catatggaag atggcgtccc
     4081 tttgactgag ttgactcaca gtgactgtga atgtctcctg tgccaaatct ttggaagtga
     4141 gtatgaagcc ggcaaaataa ggtttgaaga tctcgtattc gaaagtgacc cggaacctgt
     4201 gaccttcgat catgtggcca tcgatagatt cactggcggt gcagctgata agaagaaatt
     4261 cgatgattcc cctctgcccg gtagccctgc aagaccgctc atgttgaaag gctccttctg
     4321 gatccgcagg gacgttctcg aggacgaaga gtactgtaag gcactcggta aggctcttgc
     4381 agatgtgaat aatggccttt atcccctcgg tggaaagagc gccatcggct acggacaggt
     4441 caagagtctg ggtataaagg gagatgataa gaggatttct cgcctcatga atcctgcctt
     4501 tgatgagaca gatgtagccg ttccagaaaa gcccaaaact gatgccgagg ttcgcatcga
     4561 ggcagagaaa gtatattacc cacactattt cgtcgaaccc cataagaagg tggaacgcga
     4621 ggagaaaccc tgtggtcatc aaaagttcca cgaggggcga ctgacaggta aaattcggtg
     4681 taagctcatt accaagacac ccctcatcgt cccagatact agtaatgacg atttcttcag
     4741 acctgcggat aaagaagctc ggaaggaaaa ggacgaatat cataaatcat atgctttctt
     4801 cagacttcat aaacaaatca tgattcccgg gagcgaattg agaggaatgg tgagtagtgt
     4861 ctacgaaact gtgacaaatt cttgcttcag gatatttgat gagactaaac ggttgtcatg
     4921 gcggcgtagc gtgtcaccgt accgaactgt agacgatagg atgatcggca aacgtatgag
     4981 cgccgatctg cggccgtgcc atggagattg ggtggaagat ggtgatctca gtgccttgaa
     5041 tgcatatccc gagaaaagac tcctcttgcg ccaccccaaa ggactctgcc ctgcttgccg
     5101 gctctttgga accggatctt acaagggcag agtcaggttt ggattcgcgt cactcgaaaa
     5161 cgatccggag tggctgatcc caggcaagaa tcccggcgat ccgtttcacg gcgggccggt
     5221 gatgctctca ttgttggaac ggcctcgccc gacttggagt ataccgggat ccgacaataa
     5281 gtttaaagtg cctggcagaa agttttacgt ccaccaccac gcctggaaaa ccattaagga
     5341 cgggaaccat cccacaacag gcaaagctat tgaacaaagc cctaataacc gcactgtaga
     5401 agctctcgcc ggcgggaatt cctttagctt cgaaattgcc tttgagaacc tgaaagaatg
     5461 ggagctgggt ttgctcatcc acagcctgca actcgaaaag ggtctggcgc ataaacttgg
     5521 aatggcaaag tctatgggat ttggttcagt tgaaattgac gtcgaatcag tgcgcctgag
     5581 aaaagattgg aagcaatggc ggaatggcaa ttccgaaatt cccaactggt tgggaaaagg
     5641 atttgctaaa ctgaaggaat ggttccggga cgagctcgat tttatagaaa atcttaagaa
     5701 acttctttgg tttcctgagg gcgaccaagc accccgggtt tgctacccca tgctgcgaaa
     5761 gaaggacgat cctaatggga atagcggtta cgaagaactc aaagacgggg aattcaagaa
     5821 agaagatcgg cagaagaagc tgaccacgcc gtggacaccg tgggcataag tcgacaagct
     5881 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
     5941 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     6001 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
     6061 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6481 tttaacgcga attttaacaa actagtaacg tttacaattt caggtggcac ttttcgggga
     6541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6601 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     6661 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     6721 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     6781 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     6841 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
     6901 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     6961 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     7021 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tgttttcacc
     7081 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     7141 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     7201 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     7261 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     7321 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     7381 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     7441 attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt
     7501 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7561 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7621 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7681 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7741 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7801 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7861 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7921 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7981 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8041 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8101 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8161 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8221 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8281 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     8341 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
     8401 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     8461 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     8521 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     8581 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     8641 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     8701 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     8761 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     8821 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     8881 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     8941 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     9001 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     9061 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     9121 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     9181 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg
     9241 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     9301 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     9361 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     9421 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     9481 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atga
//
