LOCUS       sequence-418039-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production and purification of catalytically inactive SynCas.v10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Brogan DJ, Dalla Benetta E, Wang T, Lin CP, Chen F, Li H, Lin C, 
            Komives EA, Akbari OS
  TITLE     Synthetic type III-E CRISPR-Cas effectors for programmable 
            RNA-targeting
  JOURNAL   bioRxiv 2024.02.23.581838; doi: 
            https://doi.org/10.1101/2024.02.23.581838
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.23.581838; doi: https://doi.org/10.1101/2024.02.23.581838"
COMMENT     Sequence Label: T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..1111)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1073..1092
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1112..1189)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1395..1414
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1498..1517
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1498..1516
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1517..1541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1556..1578
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1593..1637
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1644..1661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1671..1688
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1692..1985
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGISIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             5817..5834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5887..5905)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5901..5948
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5985..6440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6072..6091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6282..6303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6533..7348)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6533..7348)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7256..7275
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7340..7359
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7470..8058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7959..7978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8212..8229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8244..8383
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8309..8328)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8469..8491
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8485..8676)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
       61 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      121 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
      181 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
      241 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
      301 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
      361 gcgccatctg atcgttggca accagcatcg cagtgggaac gataccctca ttcagcattt
      421 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
      481 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
      541 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
      601 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
      661 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
      721 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
      781 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
      841 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
      901 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
      961 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     1021 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     1081 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     1141 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     1201 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     1261 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     1321 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     1381 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     1441 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
     1501 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
     1561 ttaactttaa gaaggagata taccccatgg gcggtctgaa cgacatcttc gaggctcaga
     1621 aaatcgaatg gcacgaaagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
     1681 gcggcagcca tatgtcggac tcagaagtca atcaagaagc taagccagag gtcaagccag
     1741 aagtcaagcc tgagactcac atcaatttaa aggtgtccga tggatcttca gagatcttct
     1801 tcaagatcaa aaagaccact cctttaagaa ggctgatgga agcgttcgct aaaagacagg
     1861 gtaaggaaat ggactcctta agattcttgt acgacggtat tagcattcaa gctgatcaga
     1921 cccctgaaga tttggacatg gaggataacg atattattga ggctcacaga gaacagattg
     1981 gtggaagtat gacgactact atgaagattt caattgaatt cctcgagccg tttcggatga
     2041 ccaaatggca ggaaagcact aggcgaaaca agaacaacaa ggaatttgtt cgcggccaag
     2101 cttttgcaag atggcataga aataagaagg ataacacaaa agggcggcct tacataactg
     2161 ggacattgct gcggtccgca gtaattagaa gcgcggaaaa cctgctgaca ttgagtgatg
     2221 ggaaaattag cgaaaagaca tgttgtcctg ggaaatttga tacggaagat aaagacaggt
     2281 tgctgcaatt gaggcagcgg tccacccttc gttggaccga taagaacccc tgtccagata
     2341 acgctgaaac ttactgcccg ttttgcgaac ttctcgggag atccggtaac gatggaaaga
     2401 aggctgagaa gaaggattgg cgctttcgta ttcattttgg caatctttcc cttccaggga
     2461 agcccgattt cgacgggcca aaagctatag gcagccaacg ggtacttaac agggtcgatt
     2521 tcaaatccgg taaggcccat gacttcttta aggcttacga agtcgaccat actaggtttc
     2581 cccgcttcga aggggagatt accatagata ataaagtcag cgctgaagcc aggaaactgc
     2641 tctgtgattc tctcaagttt acggatcggc tgtgtggagc tctgtgcgta atcaggttcg
     2701 atgaatatac accagcagcc gatagtggga aacaaaccga aaatgtccaa gcagaaccga
     2761 atgctaatct cgctgaaaag accgcagagc aaattattag cattctggac gataataaga
     2821 aaaccgaata tacccgcctg cttgctgatg ctattcgttc actccgccgc tctagtaaac
     2881 ttgtcgctgg cttgccaaag gatcatgacg ggaaggatga ccattacctc tgggatatcg
     2941 gcaagaagaa gaaagacgaa aactctgtca ctattaggca aatccttacg acctcagcag
     3001 acaccaagga actcaagaat gccggaaaat ggagagaatt ctgcgagaag ctgggtgaag
     3061 cgctgtacct caagagtaaa gacatgagtg gcggcctgaa aattactcgg agaatactgg
     3121 gcgatgctga attccatgga aagcccgatc ggcttgaaaa gagccggtct gtgtcaatcg
     3181 gatctgtgtt gaaagaaact gtggtatgcg gcgaactggt cgctaaaacg cccttcttct
     3241 tcggagcgat agacgaagat gcaaaacaaa ccgcactgca agtactcctc actcccgata
     3301 acaagtatag actgccaaga agcgccgtgc gaggtatact ccgtcgagat cttcaaacct
     3361 attttgatag cccatgcaat gctgagttgg gtggacggcc atgcatgtgt aagacgtgta
     3421 ggattatgag agggatcacg gtgatggatg cgcgcagtga gtacaatgcc ccgccagaaa
     3481 taaggcatcg tacccgcatt aatcccttca caggcacggt tgcggaaggt gccctgttta
     3541 atatggaagt agcccccgag gggattgtct ttccatttca actccggtac cggggctctg
     3601 aagatgggct gcccgatgca ctgaaaacgg tgttgaaatg gtgggctgag gggcaggcat
     3661 tcatgagtgg cgctgcctca accgggaagg gccgattccg gatggaaaat gctaaatatg
     3721 aaacgctgga tctgagcgac gagaatcaaa ggaatgacta tcttaagaat tggggatggc
     3781 gtgacgagaa ggggctcgag gaactgaaga aacgactgaa ctcaggtctg ccagagcccg
     3841 gtaattatag ggatccaaaa tggacaaaag tagggtatga gattaaaata cagtcgcccc
     3901 ttcttagcaa cgatcctatc gcagcattgt tcgacactgc aaaccacgca gcggtcgcgt
     3961 ataagaaacg agtgtgggag aacggacgta tcatagagcg acacactttc aaaggagagt
     4021 ctatacgagg ggtgctgcgc actgcacttg ggcgtgagta cggattgttc gaattaaaac
     4081 acgaagactg tccttgtagt ctttgcacaa ttttcggtaa cgaacacgaa gcgggcaaaa
     4141 tacgtttcga agacttagaa atcgaggacg gtgttgccga aaagcacttc gatcacgttg
     4201 ctatcgatcg cttcacaggc ggagccgtag acaagaagaa attcgacgat ttctctttag
     4261 ctggttcccc gggaaagccc attaaactca aagggtgctt ttggttgcgt gcggatatat
     4321 ttagtgacga gaagaaggac gagaagaaga aaataataaa cgctctttgt gacgtgcgtg
     4381 atggcttgta ccctctcggt ggcaagggcg gtgtcggata cgggtgggta aaagagctga
     4441 gaataacgga cggcccagac gattttaagc tcccggaacc gaaatgtaag gaaattccgc
     4501 cagacggtac tccaagcgat tacccttttc cgcagttaaa gccactgcaa acagagaagg
     4561 ataaagttta ctcgccccac tactttctga aaccagcaga gaagaaagtt gaccggaact
     4621 gggaaaagcg gctgatcgga cacgagcgtt tcgataagga cctgttgact ggtaaaattg
     4681 agtgtacact gaaaacgctg agccctttaa taataccgga cagtgaggtc gttacggaga
     4741 acggaaacgg ccacaagtct taccagttct tcagattaaa taatgaaatc atcattccgg
     4801 gaagcgaaat tcggggtatg atcagttccg tgtacgaagc acttactaac tcttgcttcc
     4861 gtgtcgtgga cgacatcatc ccggtggtga tctcccgtag cgtgtcaccg taccagatcg
     4921 ctcagaagct gcccagtccg gatctgcgtc cttgcgagcg tgagattctt gaggactcag
     4981 aaattggatc tttagacaag tgttcagaga aacgcctttt ccgcctgcat gaagacggtc
     5041 tttgcccatc gtgtcgtctg ttcggcacta cacattataa gggacgcgtt cggttcggct
     5101 tcgcaaagca cgagggcggc gaaaagtggc tcatggacga caagactaag gacgagtatc
     5161 tgactttacc gttgttggaa cggccgcgcc caacatggag catgccaagc gacgaagacg
     5221 acgtaccggg acggaaattc tacgtacacc acaatggttg ggagaaagtt aagcaagata
     5281 gtttagataa taaaacgagc aaaaccgaga ataatcgaag cgtggaacca ctcgataagg
     5341 ataacctgtt taaattcgac gtgttcttcg agaacctgga accctgggaa cttggtttgc
     5401 ttctctactc attagaattg gaagaggggt tagcccacaa gtttggaatg gcaaaagcgt
     5461 tcggcttcgg ctcgacaaag attgacgccg acaaaatact gttacgtgag gctccgaacg
     5521 agtggcaaga tgctaccaat cgtaaggacg acatgttacg caagggcttc ggcgagctga
     5581 catcctggtt cgacaaggat tgggacaaag tcgagcacat agacggtctg cgttccttac
     5641 ttcagattcc ggaagcgctt tcaagagccg aagtgcgcta cccggacctt acggaagacg
     5701 acccagagaa gcccccgggc tacgtgcaat tgtctgataa gaaagagggc cgcaagtact
     5761 tgatccgtcc ctggcaacct tggtactaag tcgacaagct tgcggccgca ctcgagcacc
     5821 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     5881 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     5941 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
     6001 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6061 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6121 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6181 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6241 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6301 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6361 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6421 actagtaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
     6541 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     6601 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     6661 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     6721 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     6781 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     6841 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     6901 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     6961 ggcgcaggaa cactgccagc gcatcaacaa tgttttcacc tgaatcagga tattcttcta
     7021 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     7081 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     7141 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     7201 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     7261 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     7321 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     7381 acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8221 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     8281 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8341 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     8401 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     8461 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     8521 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     8581 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     8641 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     8701 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     8761 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     8821 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
     8881 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca
     8941 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt
     9001 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg
     9061 tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag
     9121 gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc
     9181 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
     9241 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat
     9301 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
     9361 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     9421 caagggcatc ggtcgagatc ccggtgccta atgagt
//
