LOCUS       sequence-418051-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For curing of Orbit-integrated plasmids and associated drug marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, 
            Sassetti CM
  TITLE     ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial 
            Chromosomes.
  JOURNAL   MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 
            10.1128/mBio.01467-18.
  PUBMED    30538179
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2018-12-11"; volume: "9(6). pii: mBio.01467-18. doi"; pages: 
            " 10.1128/mBio.01467-18"
COMMENT     Sequence Label: pKM512
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             643..1266
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(716..735)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      1330..1918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1819..1838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2072..2089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2179..2206
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             3639..5141
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      5176..5222
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5290..5337
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5356..5730
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      5772..5858
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5946..5962)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5947..5963)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(9129..9215,9216..10140,1..410)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg
       61 actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg
      121 gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt
      181 taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc
      241 aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag
      301 acaaacaatc aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg
      361 ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa gcggccgctg
      421 attagctaag cagaaggcca tcctgacgga tgcctttttc gctttaatac tgcatgcact
      481 ctagaaatat tggatcgtcg caccgggtta agcctggagt gcggtggtgc ctggtcggca
      541 ttttcgcagt cgagggctct cgtgtagcct gggcgagttg ccgacgcagg cgaccctcct
      601 gccacggatc cgcatggtac catcaggagg aatcacttcg caatgtctag attagataaa
      661 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
      721 acccgtaaac tcgcccagaa gcttggtgta gagcagccta cattgtattg gcatgtaaaa
      781 aataagcggg ccctgctcga cgccttagcc attgagatgt tagataggca ccatactcac
      841 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
      901 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
      961 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     1021 ctagagaacg cgttatatgc actcagcgct gtggggcatt ttaccttagg ttgcgtattg
     1081 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     1141 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     1201 ttattcggcc ttgaattgat catctgcgga ttagaaaaac aacttaaatg tgaaagtggg
     1261 tcttaatgac catgggctag atttaaatca ctgagcgtca gaccccgtag aaaagatcaa
     1321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1381 accgctacca gcggtggttt ttttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctagaa
     2161 ctagttgatt agctaagcag aaggccatcc tgacggatgg cctttttgcg tttaatactg
     2221 tttaaactct agaaatattg gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc
     2281 cgcgacgtga tgatcggccg catccccacg gtgctgcgca gtgagctcta cgccatcccg
     2341 gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct gacgagcggg
     2401 agaactccct atcagtgata gagtttgtcc tccctatcag tgatagatag gctctgggag
     2461 tacccgtgtg tacgaccagc acggcataca tcatttcgac gccgagagat tcgccgcccg
     2521 aaatgagcac gatccgcatg cttaattaag aaggagatat acatatgact cagcgtatcg
     2581 tctttctacc cgatactcag ttgcctttcg aggcgcgcaa agagatgcaa gcggtcatcc
     2641 gcttcatcgg ggatgtccag ccgtacggcg tggtacatat cggtgacgtc ctagacctgc
     2701 cgcagccctc gcgctggaac cggggaacca agggcgagtt cgagggctcg gtgtaccgcg
     2761 acgcggacta cgccaagaag aacctgatgg agccgctgcg caaggtctac gacggctgga
     2821 tcgggatgca cgagggcaac cacgatctgc gagcccgcga gtacctggcc aagaacgcac
     2881 cggccctgga gggtacgcac gctttcgaca tcgacgtgct gctcgacttc gacgggttcg
     2941 gtgtggagct gctgcctgac ttctacgaca tcgctccggg ctggatctcc actcacgggc
     3001 acatgggcaa gatgacgcta tcccagatcg ccggatcgac agcgctcaac ggtgccaaga
     3061 agttcggcaa gtccgtggtc tgcggccaca cgcaccggca ggctgtcgtc tcgcactcgt
     3121 tcggggacgg cggctcggtg cgcaagaccg tcaccggcat ggaagtcggg cacctgatgg
     3181 acatgaagaa ggccaactat ctaaagggcg gagccgggaa ctggcagatg ggcttcggga
     3241 tgctcacggt cgacggcaag catgtcaagg ctgagatcgt cccgatcctg ggaggcaagt
     3301 tcaccgttga cggccaggtc tgggaagtct gagcgccccg gcgaattccg acgcggtggt
     3361 tgatctgcgc tagtggaggc gcccgacgtg cagatggcca atcccgatca gctgaagcgc
     3421 ctcggcgaga tccagaaggc cgaaaagtac aacgacgccg attggttcaa gttcctcgcc
     3481 gattcggccg gcgtcaaagc cacgcgcgcc gccgacctca cgttcgacga ggcgaaggct
     3541 gtcatcgaca tgttcgacgg gcccaacgca tgagcgcccc ggcgaattcc gacgcggtgg
     3601 ttgatctgcg ctagtggagg cgccagaggc acttcgagat gagagccctg gtagtcatcc
     3661 gcctgtcccg cgtcaccgat gctacgactt caccggagcg tcagctggag tcttgccagc
     3721 agctctgcgc ccagcgcggc tgggacgtcg tcggggtagc ggaggatctg gacgtctccg
     3781 gggcggtcga tccgttcgac cggaagcgca gaccgaacct ggcccggtgg ctagcgttcg
     3841 aggagcaacc gttcgacgtg atcgtggcgt accgggtaga ccggttgacc cgatcgatcc
     3901 ggcatctgca gcagctggtc cactgggccg aggaccacaa gaagctggtc gtctccgcga
     3961 ccgaagcgca cttcgatacg acgacgccgt ttgcggcggt cgtcatcgcg cttatgggaa
     4021 cggtggcgca gatggaatta gaagcgatca aagagcggaa ccgttcggct gcgcatttca
     4081 atatccgcgc cgggaaatac cgaggatccc tgccgccgtg gggatacctg cctacgcgcg
     4141 tggacgggga gtggcggctg gtgccggacc ctgtgcagcg agagcgcatc ctcgaggtgt
     4201 atcaccgcgt cgtcgacaac cacgagccgc tgcacctggt ggcccacgac ctgaaccggc
     4261 gtggtgtcct gtcgccgaag gactacttcg cgcagctgca aggccgcgag ccgcagggcc
     4321 gggagtggtc ggctaccgcg ctgaagcgat cgatgatctc cgaggcgatg ctcgggtacg
     4381 cgactctgaa cggtaagacc gtccgagacg acgacggagc cccgctggtg cgggctgagc
     4441 cgatcctgac ccgtgagcag ctggaggcgc tgcgcgccga gctcgtgaag acctcccggg
     4501 cgaagcccgc ggtgtctacc ccgtcgctgc tgctgcgggt gttgttctgc gcggtgtgcg
     4561 gggagcccgc gtacaagttc gccgggggag gacgtaagca cccgcgctac cgctgccgct
     4621 cgatggggtt cccgaagcac tgcgggaacg gcacggtggc gatggccgag tgggacgcgt
     4681 tctgcgagga gcaggtgctg gatctgctcg gggacgcgga gcgtctggag aaagtctggg
     4741 tagccggctc ggactccgcg gtcgaactcg cggaggtgaa cgcggagctg gtggacctga
     4801 cgtcgctgat cggctccccg gcctaccggg ccggctctcc gcagcgagaa gcactggatg
     4861 cccgtattgc ggcgctggcc gcgcggcaag aggagctgga gggtctagag gctcgcccgt
     4921 ctggctggga gtggcgcgag accgggcagc ggttcgggga ctggtggcgg gagcaggaca
     4981 ccgcggcaaa gaacacctgg cttcggtcga tgaacgttcg gctgacgttc gacgtccgcg
     5041 gcgggctgac tcgcacgatc gacttcgggg atctgcagga gtacgagcag catctcaggc
     5101 tcggcagcgt ggtcgaacgg ctacacaccg ggatgtcgta ggttaacaac tagcgtacga
     5161 tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     5221 atgccatcat ggccgcggga cgtcgggggg gggggaaagc cacgttgtgt ctcaaaatct
     5281 ctgatgtttg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     5341 gtgaggaact aagccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     5401 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     5461 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     5521 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     5581 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctctgggcc ggccatgacc
     5641 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     5701 gtgcacttcg tggccgagga gcaggactga atgcatctct ccccatgcga gagtagggaa
     5761 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     5821 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     5881 ttgcgtcaga tatcaagctt tagctaatta attggggacc ctagaggtcc ccttttttat
     5941 tttatcgata ccgtcgacct cgaggggggg cccccgggga tcatcgagcc gagaacgtta
     6001 tcgaagttgg tcatgtgtaa tcccctcgtt tgaactttgg attaagcgta gatacaccct
     6061 tggacaagcc agttggattc ggagacaagc aaattcagcc ttaaaaaggg cgaggccctg
     6121 cggtggtgga acaccgcagg gcctctaacc gctcgacgcg ctgcaccaac cagcccgcga
     6181 acggctggca gccagcgtaa ggcgcggctc atcgggcggc gttcgccacg atgtcctgca
     6241 cttcgagcca agcctcgaac acctgctggt gtgcacgact cacccggttg ttgacaccgc
     6301 gcgcggccgt gcgggctcgg tggggcggct ctgtcgccct tgccagcgtg agtagcgcgt
     6361 acctcacctc gcccaacagg tcgcacacag ccgattcgta cgccataaag ccaggtgagc
     6421 ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc cgcgcattca ggcggcaggg
     6481 ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc ggctctcagc ggcccggaaa
     6541 cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca ggtggttggg ggtgctcggc
     6601 tgtcgctggt gttccaccac cagggctcga cgggagagcg ggggagtgtg cagttgtggg
     6661 gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg gaccatacac cggtgattaa
     6721 tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa tttgagcagc tctggctgcc
     6781 gtactggccg ctggcaagcg acgatctgct cgaggggatc taccgccaaa gccgcgcgtc
     6841 ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt
     6901 ggacgtagac catccagacg cagcgctccg agcgctcagc gcccgggggt cccatccgct
     6961 gcccaacgcg atcgtgggca atcgcgccaa cggccacgca cacgcagtgt gggcactcaa
     7021 cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg
     7081 cgccgaaggc cttcggcgcg ccgtcgacgg cgaccgcagt tactcaggcc tcatgaccaa
     7141 aaaccccggc cacatcgcct gggaaacgga atggctccac tcagatctct acacactcag
     7201 ccacatcgag gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc agcagaccac
     7261 gtacaaagcg gctccgacgc cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt
     7321 gtgggcctat cgtcccgccc tcatgcggat ctacctgccg acccggaacg tggacggact
     7381 cggccgcgcg atctatgccg agtgccacgc gcgaaacgcc gaattcccgt gcaacgacgt
     7441 gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc gccaacagca tttggcgttg
     7501 gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg ccacactcag
     7561 tgcgcgccag tcggccatct cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt
     7621 tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg ctatgagcga cggctacagc
     7681 gacggctaca accggcagcc gactgtccgc aaaaagcggc gcgtgaccgc cgccgaaggc
     7741 gctcgaatca ccggactatc cgaacgccac gtcgtccggc tcgtggcgca ggaacgcagc
     7801 gagtggctcg ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca cgacgacgag
     7861 ggccactctt ggccgcaaac ggccaaacat ttcgggctgc atctggacac cgttaagcga
     7921 ctcggctatc gggcgaggaa agagcgtgcg gcagaacagg aagcggctca aaaggcccac
     7981 aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt cgcgggggtt
     8041 ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga cgctagtttt
     8101 ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg aataccagcc
     8161 agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct gaatatcggg
     8221 gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg ttgtagtgcc
     8281 tgtggtggca tccgtggcca ctctcgttgc acggttcgtt gtgccgttac aggccccgtt
     8341 gacagctcac cgaacgtagt taaaacatgc tggtcaaact aggtttacca acgatacgag
     8401 tcagctcatc tagggccagt tctaggcgtt gttcgttgcg cggttcgttg cgcatgtttc
     8461 gtgtggttgc tagatggctc cgcaaccaca cgcttcgagg ttgagtgctt ccagcacggg
     8521 cgcgatccag aagaacttcg tcgtgcgact gtcctcgttg atccttgccg agctgggatg
     8581 gaagcccggc cgacccaccc tggaggagat gatcgaggat gccagggcct ttcacgcccg
     8641 ccgctgctga gcgtccgccg ccgggcccgc accgccgtcg gccggcccgc tccgggctcg
     8701 cagcagcggg cttcggcgcg ggcccggggc tcccgggccg ccgggcgggg ctccgcccgg
     8761 cggccgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt
     8821 ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga
     8881 ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat
     8941 cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta
     9001 tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc
     9061 catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga
     9121 catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg
     9181 cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata
     9241 aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc
     9301 aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt
     9361 ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg
     9421 caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg
     9481 acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa
     9541 acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag
     9601 accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat
     9661 tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta
     9721 acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct
     9781 ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca
     9841 gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat
     9901 acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat
     9961 ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc
    10021 tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc
    10081 taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa
//
