LOCUS       sequence-418059-       10812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid containing a multiple cloning site (MCS) 
            under the spleen focus-forming virus (SFFV) promoter and expressing 
            Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10812)
  TITLE     Plasmid DNA with two promoters
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-SFFV-MCS-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..10812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(2564..2584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2982..2993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4032..4051
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4225..4234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4231..4233,4234..4236,4237..4950)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4276..4297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4537..4556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4884..4905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5045..5278
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5303..5321)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5304..5321)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5611..5630)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5844..5863)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5963..5985
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6023..6041)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6109..6213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6214..6282,6283..7074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6432..6451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7245..7833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7734..7753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7987..8004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8074..8094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8079..8408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8259..8394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9542..9607
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9737..9757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10182..10316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10219..10238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10273..10292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10484..10812,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatcccgta cggtgaacgc gtggcgttac atatgttaat taagtttaaa cgcggccgcg
     2461 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     2521 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2581 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2641 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2701 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2761 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2821 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2881 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2941 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3001 accttccttc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     3061 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     3121 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     3181 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     3241 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     3301 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     3361 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
     3421 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     3481 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
     3541 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
     3601 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     3661 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     3721 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     3781 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     3841 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     3901 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     3961 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     4021 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     4081 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     4141 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc
     4201 ttggtaccga gctcggatca attcgccacc atggtgagca agggcgagga gctgttcacc
     4261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
     4441 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     4681 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     4741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4921 actctcggca tggacgagct gtacaagtaa ctcgagggaa ttaattcgag ctcggtacct
     4981 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5041 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5101 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5161 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5221 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     5281 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     5341 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     5401 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     5461 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     5521 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     5581 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     5641 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     5701 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     5761 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     5821 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5881 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5941 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6001 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6061 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6181 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6241 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6301 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6361 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6421 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6481 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6541 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6601 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6661 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6721 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6781 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6841 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6901 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6961 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7021 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7081 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7141 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7381 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7861 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7921 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7981 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8041 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     8101 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8161 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8221 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8281 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8341 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8401 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     8461 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     8521 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     8581 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     8641 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     8701 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     8761 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     8821 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     8881 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     8941 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     9001 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     9061 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     9121 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     9181 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     9241 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     9301 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     9361 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     9421 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     9481 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     9541 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     9601 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     9661 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     9721 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     9781 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     9841 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     9901 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     9961 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
    10021 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
    10081 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    10141 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    10201 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10261 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10321 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10381 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10441 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    10501 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10561 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    10621 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    10681 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    10741 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    10801 ttcaagaact gc
//
