LOCUS       sequence-418073-       10802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid containing a multiple cloning site (MCS) 
            under the spleen focus-forming virus (SFFV) promoter and expressing 
            mCherry under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  TITLE     Plasmid DNA with two promoters
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-SFFV-MCS-WPRE-UbC-mCherry
FEATURES             Location/Qualifiers
     source          1..10802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(2563..2583)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2981..2992)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4031..4050
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4224..4233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4230..4940
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4375..4393)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4643..4662
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5035..5268
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5293..5311)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5294..5311)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5601..5620)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5834..5853)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5953..5975
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6013..6031)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6099..6203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6204..6272,6273..7064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6422..6441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7235..7823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7724..7743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7977..7994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8064..8084)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8069..8398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8249..8384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9532..9597
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9727..9747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10172..10306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10209..10228)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10263..10282
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10474..10802,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatcccgtac ggtgaacgcg tggcgttaca tatgttaatt aagtttaaac gcggccgcga
     2461 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     2521 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2581 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2641 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2701 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2761 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2821 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2881 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2941 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3001 ccttccttcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     3061 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     3121 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     3181 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     3241 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     3301 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     3361 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     3421 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     3481 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     3541 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     3601 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     3661 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     3721 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     3781 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     3841 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     3901 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     3961 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     4021 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     4081 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     4141 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct
     4201 tggtaccgag ctcggatcaa ttcgccacca tggtgagcaa gggcgaggag gataacatgg
     4261 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     4321 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4381 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     4441 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     4501 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     4561 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     4621 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4681 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     4741 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     4801 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     4861 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     4921 tggacgagct gtacaagtaa ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     4981 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5041 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5101 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5161 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5221 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     5281 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     5341 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     5401 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     5461 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     5521 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     5581 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     5641 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     5701 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     5761 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     5821 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5881 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5941 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6001 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6061 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7981 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8041 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8101 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8161 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8221 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8281 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8401 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     8461 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     8521 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     8581 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     8641 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     8701 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     8761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     8821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     8881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     8941 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     9001 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     9061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     9121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     9181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     9241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     9301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     9361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     9421 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     9481 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     9541 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     9601 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     9661 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     9721 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     9781 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     9841 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     9901 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     9961 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    10021 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    10081 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    10141 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    10201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10261 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10321 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10381 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10441 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
    10501 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
    10561 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
    10621 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
    10681 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
    10741 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
    10801 gc
//
