LOCUS       sequence-418076-        1358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of gene  in mammalian cells by retroviral transduction .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pMSCV-Myc-Caspase-5
FEATURES             Location/Qualifiers
     source          1..1358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..47
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             329..340
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcggccgcgc caccatggaa caaaaactca tctcagaaga ggatctgatg gctgaagaca
       61 gtggcaaaaa aaaaaggcgt aagaattttg aagctatgtt caaaggtatc cttcagagtg
      121 gattggataa cttcgtgata aaccacatgc taaagaacaa cgtggctgga caaacatcta
      181 tccagaccct agtacctaat acggatcaaa agtcaaccag tgtaaaaaaa gacaaccaca
      241 aaaaaaaaac agttaagatg ttggaatacc tgggcaaaga tgttcttcat ggtgttttta
      301 attatttggc aaaacacgat gttctgacat tgaaggaaga ggaaaagaaa aaatattatg
      361 ataccaaaat tgaagacaag gccctgatct tggtagactc tttgcgaaag aatcgcgtgg
      421 ctcatcaaat gtttacccaa acacttctca atatggacca aaagatcacc agtgtaaaac
      481 ctcttctgca aatcgaggct ggaccacctg agtcagcaga atctacaaat atactcaaac
      541 tttgtcctcg tgaagaattc ctgagactgt gtaaaaaaaa tcatgatgag atctatccaa
      601 taaaaaagag agaggaccgc agacgcctgg ctctcatcat atgcaataca aagtttgatc
      661 acctgcctgc aaggaatggg gctcactatg acatcgtggg gatgaaaagg ctgcttcaag
      721 gcctgggcta cactgtggtt gacgaaaaga atctcacagc cagggatatg gagtcagtgc
      781 tgagggcatt tgctgccaga ccagagcaca agtcctctga cagcacgttc ttggtactca
      841 tgtctcatgg catcctagag ggaatctgcg gaactgcgca taaaaagaaa aaaccggatg
      901 tgctgcttta tgacaccatc ttccagatat tcaacaaccg caactgcctc agtctaaagg
      961 acaaacccaa ggtcatcatt gtccaggcct gcagaggtga aaaacatggg gaactctggg
     1021 tcagagactc tccagcatcc ttggcactca tctcttcaca gtcatctgag aacctggagg
     1081 cagattctgt ttgcaagatc cacgaggaga aggacttcat tgctttctgt tcttcaacac
     1141 cacataacgt gtcctggaga gaccgcacaa ggggctccat cttcattacg gaactcatca
     1201 catgcttcca gaaatattct tgctgctgcc acctaatgga aatatttcgg aaggtacaga
     1261 aatcatttga agttccacag gctaaagccc agatgcccac catagaacga gcaaccttga
     1321 caagagattt ctacctcttt cctggcaatt gagtcgac
//
