LOCUS       sequence-418077-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28_Caspase-4_p20_C258A
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc gctggaccac ctgagtcagg agaatctaca
      121 gatgccctca agctttgtcc tcatgaagaa ttcctgagac tatgtaaaga aagagctgaa
      181 gagatctatc caataaagga gagaaacaac cgcacacgcc tggctctcat catatgcaat
      241 acagagtttg accatctgcc tccgaggaat ggagctgact ttgacatcac agggatgaag
      301 gagctacttg agggtctgga ctatagtgta gatgtagaag agaatctgac agccagggat
      361 atggagtcag cgctgagggc atttgctacc agaccagagc acaagtcctc tgacagcaca
      421 ttcttggtac tcatgtctca tggcatcctg gagggaatct gcggaactgt gcatgatgag
      481 aaaaaaccag atgtgctgct ttatgacacc atcttccaga tattcaacaa ccgcaactgc
      541 ctcagtctga aggacaaacc caaggtcatc attgtccagg ccgcaagagg tgcaaaccgt
      601 ggggaactgt gggtcagaga ctgagcggcc gc
//
