LOCUS       sequence-418080-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Caspase-11_rat_catalytic domain
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc gacactctca agctttgttc ctccgaggag
      121 ttcacaaggc tgtgcagaga aaagaagcaa gaaatttacc caataaagga gaccaatggc
      181 cgtactcgaa aggctcttat catatgcaat acagagttca aacatctctc cctgagatat
      241 ggggctaaca ttgacattag tggcatgaaa ggccttcttg aagagttggg ctatgatgtg
      301 gtggtgaaag aggagcttac ggctgagggc atggaatcag aaatgaaaga ctttgctgca
      361 ctctcagagc accaaacatc agacagcaca ttcctggtgt taatgtctca tggcacattg
      421 cagggccttt gtggaacaat gcacagtgaa gcaactccag atgtgctatt atatgatact
      481 atctatcaga tatttaacaa ttgtcactgt ccaggtctac gagacaaacc caaagtcatc
      541 attgtgcagg cctgcagagg tggaaaccct ggggaagtgt ggatcagaga gtcttcagga
      601 gcccactcct acagagctgt agatctacct aggaatatgg aagctgatgc tgtccgcatg
      661 agccatgtgg agaaggactt cattgccctc tactctacaa caccacacca cttgtcctac
      721 agagacaaaa caagaggctc ttacttcatc tccaaactca tttcctgctt ccggatacat
      781 gcctgctctt gtcatctatt tgatatattt ctgaaggtgc aacaatcatt tgaaaaggca
      841 agtattaatt cccagatgcc caccattgat cgggcaacct tgacaagata tttctacctc
      901 tttcctggca attgagaatt c
//
