LOCUS       sequence-418087-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-18_dog
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atggctgcta acctaataga agacaattgc
      121 atcaaccttg tgaaaatgaa atttgttaac aatacactgt actttaaagc ggaaagtgat
      181 gaaggcctgg aatcagatta ctttggcaag cttgaaccta aactctcaat catacgaaat
      241 ttgaacgacc aagtcctctt cgttaacgag ggaaatcaac ctgtatttga ggatatgccc
      301 gattctgact gtacagataa tgcaccccat accatattta tcatctatat gtataaagat
      361 agcctcacta gaggtctggc agtaactatc tctgtgaagt ataagacaat gtctactctc
      421 tcctgtaaga acaaaactat ttcctttcag aaaatgagtc ctccggatag tatcaatgat
      481 gaaggaaatg acatcatatt ctttcagaga agtgttccag gccatgatga taagatacaa
      541 tttgagtctt cattgtacaa aggacacttt ctagcttgta aaaaagagaa cgatcttttc
      601 aaactcattt tgaaagacaa ggatgaaaat ggggataaat ccataatgtt cactgttcaa
      661 aacaagagct aggaattc
//
