LOCUS       sequence-418091-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-1-alpha
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             904..933
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atggccaaag ttccagacat gtttgaagac
      121 ctgaagaact gttacagtga aaatgaagaa gacagttcct ccattgatca tctgtctctg
      181 aatcagaaat ccttctatca tgtaagctat ggcccactcc atgaaggctg catggatcaa
      241 tctgtgtctc tgagtatctc tgaaacctct aaaacatcca agcttacctt caaggagagc
      301 atggtggtag tagcaaccaa cgggaaggtt ctgaagaaga gacggttgag tttaagccaa
      361 tccatcactg atgatgacct ggaggccatc gccaatgact cagaggaaga aatcatcaag
      421 cctaggtcag caccttttag cttcctgagc aatgtgaaat acaactttat gaggatcatc
      481 aaatacgagt tcatcctgaa tgacgccctc aatcaaagta taattcgagc caatgatcag
      541 tacctcacgg ctgctgcatt acataatctg gatgaagcag tgaaatttga catgggtgct
      601 tataagtcat caaaggatga tgctaaaatt accgtgattc taagaatctc aaaaactcaa
      661 ttgtatgtga ctgcccaaga tgaagaccaa ccagtgctgc tgaaggagat gcctgagata
      721 cccaaaacca tcacaggtag tgagaccaac ctcctcttct tctgggaaac tcacggcact
      781 aagaactatt tcacatcagt tgcccatcca aacttgttta ttgccacaaa gcaagactac
      841 tgggtgtgct tggcaggggg gccaccctct atcactgact ttcagatact ggaaaaccag
      901 gcggaacaaa aactcatctc agaagaggat ctgtaagaat tc
//
