LOCUS       sequence-418092-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-33
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             901..930
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atgaagccta aaatgaagta ttcaaccaac
      121 aaaatttcca cagcaaagtg gaagaacaca gcaagcaaag ccttgtgttt caagctggga
      181 aaatcccaac agaaggccaa agaagtttgc cccatgtact ttatgaagct ccgctctggc
      241 cttatgataa aaaaggaggc ctgttacttt aggagagaaa ccaccaaaag gccttcactg
      301 aaaacaggta gaaagcacaa aagacatctg gtactcgctg cctgtcaaca gcagtctact
      361 gtggagtgct ttgcctttgg tatatcaggg gtccagaaat atactagagc acttcatgat
      421 tcaagtatca caggaatttc acctattaca gagtatcttg cttctctaag cacatacaat
      481 gatcaatcca ttacttttgc tttggaggat gaaagttatg agatatatgt tgaagacttg
      541 aaaaaagatg aaaagaaaga taaggtgtta ctgagttact atgagtctca acacccctca
      601 aatgaatcag gtgacggtgt tgatggtaag atgttaatgg taaccctgag tcctacaaaa
      661 gacttctggt tgcatgccaa caacaaggaa cactctgtgg agctccataa gtgtgaaaaa
      721 ccactgccag accaggcctt ctttgtcctt cataatatgc actccaactg tgtttcattt
      781 gaatgcaaga ctgatcctgg agtgtttata ggtgtaaagg ataatcatct tgctctgatt
      841 aaagtagact cttctgagaa tttgtgtact gaaaatatct tgtttaagct ctctgaaact
      901 gaacaaaaac tcatctcaga agaggatctg taggaattc
//
