LOCUS       sequence-418093-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-36-alpha
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             565..594
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atggaaaaag cattgaaaat tgacacacct
      121 cagcagggga gcattcagga tatcaatcat cgggtgtggg ttcttcagga ccagacgctc
      181 atagcagtcc cgaggaagga ccgtatgtct ccagtcacta ttgccttaat ctcatgccga
      241 catgtggaga cccttgagaa agacagaggg aaccccatct acctgggcct gaatggactc
      301 aatctctgcc tgatgtgtgc taaagtcggg gaccagccca cactgcagct gaaggaaaag
      361 gatataatgg atttgtacaa ccaacccgag cctgtgaagt cctttctctt ctaccacagc
      421 cagagtggca ggaactccac cttcgagtct gtggctttcc ctggctggtt catcgctgtc
      481 agctctgaag gaggctgtcc tctcatcctt acccaagaac tggggaaagc caacactact
      541 gactttgggt taactatgct gtttgaacaa aaactcatct cagaagagga tctgtaggaa
      601 ttc
//
