LOCUS       sequence-418094-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-36-beta
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             583..612
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atgaacccac aacgggaggc agcacccaaa
      121 tcctatgcta ttcgtgattc tcgacagatg gtgtgggtcc tgagtggaaa ttctttaata
      181 gcagctcctc ttagccgcag cattaagcct gtcactcttc atttaatagc ctgtagagac
      241 acagagttca gtgacaagga aaagggtaat atggtttacc tgggaatcaa gggaaaagat
      301 ctctgtctct tctgtgcaga aattcagggc aagcctactt tgcagcttaa gcttcagggc
      361 tcccaagata acatagggaa ggacacttgc tggaaactag ttggtattca cacatgcata
      421 aacctggatg tgagagagag ctgcttcatg ggaacccttg accaatgggg aataggagtg
      481 ggtagaaaga agtggaagag ttcctttcaa catcaccatc tcaggaagaa ggacaaagat
      541 ttctcatcca tgcggaccaa cataggaatg ccaggaagga tggaacaaaa actcatctca
      601 gaagaggatc tgtaggaatt c
//
