LOCUS       sequence-418095-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-36-gamma
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             598..627
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atgagaggca ctccaggaga cgctgatggt
      121 ggaggaaggg ccgtctatca atcaatgtgt aaacctatta ctgggactat taatgatttg
      181 aatcagcaag tgtggaccct tcagggtcag aaccttgtgg cagttccacg aagtgacagt
      241 gtgaccccag tcactgttgc tgttatcaca tgcaagtatc cagaggctct tgagcaaggc
      301 agaggggacc ccatttattt gggaatccag aatccagaaa tgtgtttgta ttgtgagaag
      361 gttggagaac agcccacatt gcagctaaaa gagcagaaga tcatggatct gtatggccaa
      421 cccgagcccg tgaaaccctt ccttttctac cgtgccaaga ctggtaggac ctccaccctt
      481 gagtctgtgg ccttcccgga ctggttcatt gcctcctcca agagagacca gcccatcatt
      541 ctgacttcag aacttgggaa gtcatacaac actgcctttg aattaaatat aaatgacgaa
      601 caaaaactca tctcagaaga ggatctgtag gaattc
//
