LOCUS       sequence-418096-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
  TITLE     Structural insights into cytokine cleavage by inflammatory 
            caspase-4.
  JOURNAL   Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9.
            Epub 2023 Nov 22.
  PUBMED    37993712
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06751-9"; journalName: "Nature"; date: 
            "2023-12"; volume: "624"; issue: "7991"; pages: "451-459"
COMMENT     Sequence Label: pET28a_Pro-IL-37
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             745..774
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggaaaacc tgtattttca gggcggatcc atgtcctttg tgggggagaa ctcaggagtg
      121 aaaatgggct ctgaggactg ggaaaaagat gaaccccagt gctgcttaga agacccggct
      181 ggaagccccc tggaaccagg cccaagcctc cccaccatga attttgttca cacaagtcca
      241 aaggtgaaga acttaaaccc gaagaaattc agcattcatg accaggatca caaagtactg
      301 gtcctggact ctgggaatct catagcagtt ccagataaaa actacatacg cccagagatc
      361 ttctttgcat tagcctcatc cttgagctca gcctctgcgg agaaaggaag tccgattctc
      421 ctgggggtct ctaaagggga gttttgtctc tactgtgaca aggataaagg acaaagtcat
      481 ccatcccttc agctgaagaa ggagaaactg atgaagctgg ctgcccaaaa ggaatcagca
      541 cgccggccct tcatctttta tagggctcag gtgggctcct ggaacatgct ggagtcggcg
      601 gctcaccccg gatggttcat ctgcacctcc tgcaattgta atgagcctgt tggggtgaca
      661 gataaatttg agaacaggaa acacattgaa ttttcatttc aaccagtttg caaagctgaa
      721 atgagcccca gtgaggtcag cgatgaacaa aaactcatct cagaagagga tctgtaggaa
      781 ttc
//
