LOCUS       sequence-418104-       12141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild-type neonatal isoform of human SCN2A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Thompson CH, Potet F, Abramova TV, DeKeyser JM, Ghabra NF, Vanoye 
            CG, Millichap J, George AL Jr
  TITLE     Epilepsy-associated SCN2A (Na (V) 1.2) Variants Exhibit Diverse and 
            Complex Functional Properties.
  JOURNAL   bioRxiv. 2023 Feb 23:2023.02.23.529757. doi: 
            10.1101/2023.02.23.529757. Preprint.
  PUBMED    36865317
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Feb 23:2023.02.23.529757. doi: 10.1101/2023.02.23.529757. 
            Preprint."
COMMENT     Sequence Label: pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
FEATURES             Location/Qualifiers
     source          1..12141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          4552..4684
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    join(6159..6732,6733..6735,6736..6744)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6326..6343)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6553..6572
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6745..7440
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6878..6896)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7146..7165
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7715..7836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7752..7771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7806..7825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7843..8298)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7980..8001)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8192..8211
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8325..8429
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8431..8622
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8454..8474)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             8769..9563
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8769..9563
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8823..8842)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9433..9452
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9751..9770)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9795..9842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     repeat_region   10072..10134
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      10442..11030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10931..10950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   11160..11194
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        11533..11836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11837..12040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11991..12011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        12086..12104
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      join(12136..12141,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcacagt cagtgctggt accgccagga cctgacagct tccgcttctt taccagggaa
       61 tcccttgctg ctattgaaca acgcattgca gaagagaaag ctaagagacc caaacaggaa
      121 cgcaaggatg aggatgatga aaatggccca aagccaaaca gtgacttgga agcaggaaaa
      181 tctcttccat ttatttatgg agacattcct ccagagatgg tgtcagtgcc cctggaggat
      241 ctggacccct actatatcaa taagaaaacg tttatagtat tgaataaagg gaaagcaatc
      301 tctcgattca gtgccacccc tgccctttac attttaactc ccttcaaccc tattagaaaa
      361 ttagctatta agattttggt acattcttta ttcaatatgc tcattatgtg cacgattctt
      421 accaactgtg tatttatgac catgagtaac cctccagact ggacaaagaa tgtggagtat
      481 acctttacag gaatttatac ttttgaatca cttattaaaa tacttgcaag gggcttttgt
      541 ttagaagatt tcacattttt acgggatcca tggaattggt tggatttcac agtcattact
      601 tttgcgtatg taacagaatt tgtaaaccta ggcaatgttt cagctcttcg aactttcaga
      661 gtcttgagag ctttgaaaac tatttctgta attccaggcc tgaagaccat tgtgggggcc
      721 ctgatccagt cagtgaagaa gctttctgat gtcatgatct tgactgtgtt ctgtctaagc
      781 gtgtttgcgc taataggatt gcagttgttc atgggcaacc tacgaaataa atgtttgcaa
      841 tggcctccag ataattcttc ctttgaaata aatatcactt ccttctttaa caattcattg
      901 gatgggaatg gtactacttt caataggaca gtgagcatat ttaactggga tgaatatatt
      961 gaggataaaa gtcactttta ttttttagag gggcaaaatg atgctctgct ttgtggcaac
     1021 agctcagatg caggccagtg tcctgaagga tacatctgtg tgaaggctgg tagaaacccc
     1081 aactatggct acacgagctt tgacaccttt agttgggcct ttttgtcctt atttcgtctc
     1141 atgactcaag acttctggga aaacctttat caactgacac tacgtgctgc tgggaaaacg
     1201 tacatgatat tttttgtgct ggtcattttc ttgggctcat tctatctaat aaatttgatc
     1261 ttggctgtgg tggccatggc ctatgaggaa cagaatcagg ccacattgga agaggctgaa
     1321 cagaaggaag ctgaatttca gcagatgctc gaacagttga aaaagcaaca agaagaagct
     1381 caggcggcag ctgcagccgc atctgctgaa tcaagagact tcagtggtgc tggtgggata
     1441 ggagtttttt cagagagttc ttcagtagca tctaagttga gctccaaaag tgaaaaagag
     1501 ctgaaaaaca gaagaaagaa aaagaaacag aaagaacagt ctggagaaga agagaaaaat
     1561 gacagagtcc gaaaatcgga atctgaagac agcataagaa gaaaaggttt ccgtttttcc
     1621 ttggaaggaa gtaggctgac atatgaaaag agattttctt ctccacacca gtccttactg
     1681 agcatccgtg gctccctttt ctctccaaga cgcaacagta gggcgagcct tttcagcttc
     1741 agaggtcgag caaaggacat tggctctgag aatgactttg ctgatgatga gcacagcacc
     1801 tttgaggaca atgacagccg aagagactct ctgttcgtgc cgcacagaca tggagaacgg
     1861 cgccacagca atgtcagcca ggccagccgt gcctccaggg tgctccccat cctgcccatg
     1921 aatgggaaga tgcatagcgc tgtggactgc aatggtgtgg tctccctggt cgggggccct
     1981 tctaccctca catctgctgg gcagctccta ccagagggca caactactga aacagaaata
     2041 agaaagagac ggtccagttc ttatcatgtt tccatggatt tattggaaga tcctacatca
     2101 aggcaaagag caatgagtat agccagtatt ttgaccaaca ccatggaaga acttgaagaa
     2161 tccagacaga aatgcccacc atgctggtat aaatttgcta atatgtgttt gatttgggac
     2221 tgttgtaaac catggttaaa ggtgaaacac cttgtcaacc tggttgtaat ggacccattt
     2281 gttgacctgg ccatcaccat ctgcattgtc ttaaatacac tcttcatggc tatggagcac
     2341 tatcccatga cggagcagtt cagcagtgta ctgtctgttg gaaacctggt cttcacaggg
     2401 atcttcacag cagaaatgtt tctcaagata attgccatgg atccatatta ttactttcaa
     2461 gaaggctgga atatttttga tggttttatt gtgagcctta gtttaatgga acttggtttg
     2521 gcaaatgtgg aaggattgtc agttctccga tcattccggc tgctccgagt tttcaagttg
     2581 gcaaaatctt ggccaactct aaatatgcta attaagatca ttggcaattc tgtgggggct
     2641 ctaggaaacc tcaccttggt attggccatc atcgtcttca tttttgctgt ggtcggcatg
     2701 cagctctttg gtaagagcta caaagaatgt gtctgcaaga tttccaatga ttgtgaactc
     2761 ccacgctggc acatgcatga ctttttccac tccttcctga tcgtgttccg cgtgctgtgt
     2821 ggagagtgga tagagaccat gtgggactgt atggaggtcg ctggccaaac catgtgcctt
     2881 actgtcttca tgatggtcat ggtgattgga aatctagtgg ttctgaacct cttcttggcc
     2941 ttgcttttga gttccttcag ttctgacaat cttgctgcca ctgatgatga taacgaaatg
     3001 aataatctcc agattgctgt gggaaggatg cagaaaggaa tcgattttgt taaaagaaaa
     3061 atacgtgaat ttattcagaa agcctttgtt aggaagcaga aagctttaga tgaaattaaa
     3121 ccgcttgaag atctaaataa taaaaaagac agctgtattt ccaaccatac caccatagaa
     3181 ataggcaaag acctcaatta tctcaaagac ggaaatggaa ctactagtgg cataggcagc
     3241 agtgtagaaa aatatgtcgt ggatgaaagt gattacatgt catttataaa caaccctagc
     3301 ctcactgtga cagtaccaat tgctgttgga gaatctgact ttgaaaattt aaatactgaa
     3361 gaattcagca gcgagtcaga tatggaggaa agcaaagaga agctaaatgc aactagttca
     3421 tctgaaggca gcacggttga tattggagct cccgccgagg gagaacagcc tgaggttgaa
     3481 cctgaggaat cccttgaacc tgaagcctgt tttacagaag actgtgtacg gaagttcaag
     3541 tgttgtcaga taagcataga agaaggcaaa gggaaactct ggtggaattt gaggaaaaca
     3601 tgctataaga tagtggagca caattggttc gaaaccttca ttgtcttcat gattctgctg
     3661 agcagtgggg ctctggcctt tgaagatata tacattgagc agcgaaaaac cattaagacc
     3721 atgttagaat atgctgacaa ggttttcact tacatattca ttctggaaat gctgctaaag
     3781 tgggttgcat atggttttca agtgtatttt accaatgcct ggtgctggct agacttcctg
     3841 attgttgatg tctcactggt tagcttaact gcaaatgcct tgggttactc agaacttggt
     3901 gccatcaaat ccctcagaac actaagagct ctgaggccac tgagagcttt gtcccggttt
     3961 gaaggaatga gggttgttgt aaatgctctt ttaggagcca ttccatctat catgaatgta
     4021 cttctggttt gtctgatctt ttggctaata ttcagtatca tgggagtgaa tctctttgct
     4081 ggcaagtttt accattgtat taattacacc actggagaga tgtttgatgt aagcgtggtc
     4141 aacaactaca gtgagtgcaa agctctcatt gagagcaatc aaactgccag gtggaaaaat
     4201 gtgaaagtaa actttgataa cgtaggactt ggatatctgt ctctacttca agtagccacg
     4261 tttaagggat ggatggatat tatgtatgca gctgttgatt cacgaaatgt agaattacaa
     4321 cccaagtatg aagacaacct gtacatgtat ctttattttg tcatctttat tatttttggt
     4381 tcattcttta ccttgaatct tttcattggt gtcatcatag ataacttcaa ccaacagaaa
     4441 aagaagtttg gaggtcaaga catttttatg acagaagaac agaagaaata ctacaatgca
     4501 atgaaaaaac tgggttcaaa gaaaccacaa aaacccatac ctcgacctgc tgtaagtatc
     4561 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
     4621 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
     4681 acagaacaaa ttccaaggaa tggtctttga ttttgtaacc aaacaagtct ttgatatcag
     4741 catcatgatc ctcatctgcc ttaacatggt caccatgatg gtggaaaccg atgaccagag
     4801 tcaagaaatg acaaacattc tgtactggat taatctggtg tttattgttc tgttcactgg
     4861 agaatgtgtg ctgaaactga tctctcttcg ttactactat ttcactattg gatggaatat
     4921 ttttgatttt gtggtggtca ttctctccat tgtaggaatg tttctggctg aactgataga
     4981 aaagtatttt gtgtccccta ccctgttccg agtgatccgt cttgccagga ttggccgaat
     5041 cctacgtctg atcaaaggag caaaggggat ccgcacgctg ctctttgctt tgatgatgtc
     5101 ccttcctgcg ttgtttaaca tcggcctcct tcttttcctg gtcatgttca tctacgccat
     5161 ctttgggatg tccaattttg cctatgttaa gagggaagtt gggatcgatg acatgttcaa
     5221 ctttgagacc tttggcaaca gcatgatctg cctgttccaa attacaacct ctgctggctg
     5281 ggatggattg ctagcaccta ttcttaatag tggacctcca gactgtgacc ctgacaaaga
     5341 tcaccctgga agctcagtta aaggagactg tgggaaccca tctgttggga ttttcttttt
     5401 tgtcagttac atcatcatat ccttcctggt tgtggtgaac atgtacatcg cggtcatcct
     5461 ggagaacttc agtgttgcta ctgaagaaag tgcagagcct ctgagtgagg atgactttga
     5521 gatgttctat gaggtttggg agaagtttga tcccgatgcg acccagttta tagagtttgc
     5581 caaactttct gattttgcag atgccctgga tcctcctctt ctcatagcaa aacccaacaa
     5641 agtccagctc attgccatgg atctgcccat ggtgagtggt gaccggatcc actgtcttga
     5701 catcttattt gcttttacaa agcgtgtttt gggtgagagt ggagagatgg atgcccttcg
     5761 aatacagatg gaagagcgat tcatggcatc aaacccctcc aaagtctctt atgagcccat
     5821 tacgaccacg ttgaaacgca aacaagagga ggtgtctgct attattatcc agagggctta
     5881 cagacgctac ctcttgaagc aaaaagttaa aaaggtatca agtatataca agaaagacaa
     5941 aggcaaagaa tgtgatggaa cacccatcaa agaagatact ctcattgata aactgaatga
     6001 gaattcaact ccagagaaaa ccgatatgac gccttccacc acgtctccac cctcgtatga
     6061 tagtgtgacc aaaccagaaa aagaaaaatt tgaaaaagac aaatcagaaa aggaagacaa
     6121 agggaaagat atcagggaaa gtaaaaagta aggatccgcc cctctccctc ccccccccct
     6181 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6241 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6301 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6361 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6421 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6481 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6541 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6601 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6661 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6721 cacgatgata atatggccac aaccatggtg agcaagggcg aggcagtgat caaggagttc
     6781 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     6841 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     6901 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
     6961 ttcaccaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     7021 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     7081 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     7141 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     7201 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggaggc
     7261 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     7321 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     7381 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     7441 taacctgcag gaattcccct tcccaacagt tgcgcagcct gaatggcgaa tggagatcca
     7501 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattca
     7561 cagtcccaag gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag
     7621 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     7681 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     7741 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7801 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     7861 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     7921 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     7981 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     8041 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     8101 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     8161 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     8221 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     8281 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8401 tgcttcaata atattgaaaa aggaagaatc ctgaggcgga aagaaccagc tgtggaatgt
     8461 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8521 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8581 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgccccatg gctgactaat
     8641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8701 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     8761 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8821 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8881 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8941 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9001 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9061 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9121 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9181 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9241 tggacgaaga acatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9301 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9361 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9421 atcaggacat agcgttggct acccgtgata ttgctgaaga acttggcggc gaatgggctg
     9481 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9541 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9601 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9661 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9721 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     9781 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     9841 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac tgcctaacgc
     9901 gtttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     9961 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
    10021 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
    10081 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaac
    10141 gcgttaggca tacgcatcca ccgagacccc attggggcca atacgcccgc gtttcttcct
    10201 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
    10261 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
    10321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11101 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatttaac
    11161 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
    11221 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
    11281 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
    11341 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
    11401 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
    11461 tagataatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    11521 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    11581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    11641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    11701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    11761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    11821 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
    11881 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    11941 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
    12001 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
    12061 ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc
    12121 tccaccgcgg tggcggccgc c
//
