LOCUS       sequence-418105-       10811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing mCherry under the spleen 
            focus-forming virus (SFFV) promoter and containing a multiple 
            cloning site (MCS) under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10811)
  TITLE     Plasmid DNA with two promoters
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-SFFV-mCherry-WPRE-UbC-MCS
FEATURES             Location/Qualifiers
     source          1..10811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2413..2422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2419..3129
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2564..2582)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2832..2851
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3243..3263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3661..3672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4711..4730
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     LTR             5044..5277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5302..5320)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5303..5320)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5610..5629)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5843..5862)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5962..5984
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6022..6040)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6108..6212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6213..6281,6282..7073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6431..6450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7244..7832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7733..7752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7986..8003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8073..8093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8078..8407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8258..8393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9541..9606
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9736..9756
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10181..10315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10218..10237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10272..10291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10483..10811,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2461 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2521 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2581 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2641 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2701 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2761 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     2821 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     2881 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     2941 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3001 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3061 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3121 tacaagtaaa gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3181 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3241 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3301 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3361 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3421 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3481 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3541 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3601 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3661 tcggccctca atccagcgga ccttccttcc cgcgggcgcc cccctcctca cggcgagcgc
     3721 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     3781 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     3841 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     3901 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     3961 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     4021 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     4081 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     4141 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     4201 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     4261 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     4321 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     4381 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     4441 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     4501 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     4561 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     4621 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     4681 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     4741 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     4801 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     4861 cttttttgtt agacgaagct tggtaccgag ctcggatcaa ttcttcgaaa tcgtggccag
     4921 tttaaaccca acaacctact ggcggaccgc tcgagggaat taattcgagc tcggtacctt
     4981 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5041 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5101 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5161 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5221 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     5281 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     5341 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     5401 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     5461 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     5521 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     5581 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     5641 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     5701 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     5761 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     5821 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5881 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5941 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6001 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6061 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8041 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     8101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8161 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8221 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8281 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8341 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8401 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     8461 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     8521 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     8581 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     8641 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     8701 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     8761 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     8821 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     8881 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     8941 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     9001 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     9061 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     9121 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     9181 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     9241 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     9301 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     9361 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     9421 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     9481 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     9541 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     9601 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     9661 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     9721 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     9781 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     9841 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     9901 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     9961 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    10021 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    10081 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    10141 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    10201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10321 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    10381 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    10441 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
    10501 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
    10561 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
    10621 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
    10681 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
    10741 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
    10801 tcaagaactg c
//
