LOCUS       sequence-418106-       11712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Syt7 with C terminal HaloTag and 
            PM-targeted msGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
FEATURES             Location/Qualifiers
     source          1..11712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(76..405)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      90..225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(144..163)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     390..410
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(419..847)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(529..550)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     741..760
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    893..1117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1106..1123
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1149..1329
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1834..1850
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1835..1851
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1854..2442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1960..1971
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2369..2389
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2530..2553)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2587..2608)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3195..3216
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3523..3579)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4489..4509)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     promoter        complement(5798..6244)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    6289..6406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6704..6748)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6933..7166
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7659..7784
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7831..8011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8029..8227)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8054..8074)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8229..8608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8780..8799
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8874..8978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8979..9047,9048..9839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9197..9216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10010..10598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10499..10518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10752..10769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10886..10907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10922..10927,10928..10945,10946..10952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10960..10976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10965..10987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10984..11000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10984..11000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    11049..11170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11060..11079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11114..11133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(11300..11674)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(join(11693..11712,1..28))
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca
       61 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      121 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
      181 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      241 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
      301 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
      361 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa
      421 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      481 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      541 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      601 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
      661 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
      721 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
      781 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
      841 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
      901 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      961 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     1021 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     1081 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     1141 acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     1201 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     1261 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     1321 gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct
     1381 gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg
     1441 gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg
     1501 tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta
     1561 gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     1621 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     1681 atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga ctggaaaacc
     1741 cacctcctcc tcctcttgtg cttctagcca ggcacaatca gcattggtag ctgctgtatt
     1801 gctacttgtg attgctccat gtttttctag gtctcgaggt cgacggtatc gatgcgggga
     1861 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     1921 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     1981 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     2041 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     2101 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     2161 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     2221 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     2281 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     2341 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     2401 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     2461 gaattctagc tgaggagtgc ggccttagag ggcaacttca ttttcatagc aaaagctgtt
     2521 tgcgttctct ttatcgtcgt catccttgta gtcactggat ccgccgagag tgatcccggc
     2581 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     2641 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     2701 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctccacgt tgtggcggat
     2761 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct
     2821 gttgaagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     2881 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     2941 gtaggtgccg tcgtccttga aggtgatggt gcgctcctgg acgtagcctt cgggcatggc
     3001 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     3061 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     3121 gaacttcagg gtcagcttgc cgttggtggc atcgccctcg ccctcgccgc gcacgctgaa
     3181 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     3241 gctctcagtg ctatccatac tagatccgct ggaccccgaa ctgcccgaag aacctgaccc
     3301 ctggcgtctc cgctcccttc ttttctgcag gtacttgaag aagatgccta gcccaatgaa
     3361 aagcaggagg ccggcgacgc cccccagcac aatcagggcc attggctgaa caggtgtaga
     3421 ccatgtgggc agaacagatc ctccacctcc acttccacct ccaccgctag ccctcgcagc
     3481 actgagcaga agcagcattg cagccaccaa agagagcttc atggggcctg ggttctcctc
     3541 cacgtcccca gcttgtttaa gcaatgaaaa attagtggcc ccgctaccac cggaaatctc
     3601 cagagtagac agccagcgcg cgatctcgct gccgatcagg tccgggttgt cttcttgcag
     3661 cagattcaga cccgggccga tgtccacagc cttgcagtta ggcaggcttt tggccaggcg
     3721 agcggcttcg gccggtggga tcagaacgcc tggggtgccc cagaacagca gcttcgggac
     3781 aggggactgg tgcagccagt ccatgtattc ttcgaccagc gcgacgatgt tcgctggctc
     3841 accggcgatt ggcagctcgt ttgggaagcg ccacagtggc tcgcggtcaa caggattcag
     3901 gaacggctcg cggtaatggt ccatctcgac ttcagtcagc gggcggacga cacccatcgg
     3961 cagcgtaccc tcgataaaaa cgttctgatc gatgatcagc ttgcggccga cgtcggtggt
     4021 gcggaaggcc tggaaggtct cgcgggcaaa ttctggccat tcgtcccagg tcgggatagg
     4081 gcggttgaac tccataaatg caataccttt gacgcgctct ggattgcgct tggcccagtg
     4141 gaaacccaga gcggagcccc agtcgtgaat gaccaggacg acctcttcca gacccagggc
     4201 ttcgatgaag gcatccatga agcggacgtg gtcgtcgaag aaataaccca ggtctggttt
     4261 gtcggatttg cccataccga tcaggtctgg agcaatgcag cgatgggtcg gtgcaacatg
     4321 cgggatgatg ttgcgccaca cgtaggagga ggtcgggtta ccgtgcagga acagcacagg
     4381 ggtgccatcg cgcggaccaa catcgacgta gtgcatgcgc tcgcccagga cttccacata
     4441 atgggggtcg aatggaaagc caataccgat ttcggatcca tcgttatcgc tctgaaagta
     4501 cagatcctcg gaactgcctg aagaaccgct ggaccccgaa cttccggatc cggctttcag
     4561 ctggtgccac tgggccacag gctgccgggg acgagcgatc atgtccttcc agtgtttcac
     4621 ttcacctggt ccgctcttcc aggacaggta gatcttgccg atgacgtcgt tgcggctgag
     4681 cttgtctttg tccatgacag tgatgatgat cgtggtctcc ctcagcttct ccgtgggtat
     4741 gtcgaaggcg aaagactcat tgaagatggg gttcaggttc ctcttctttg tcacagtctt
     4801 ctttttctct acccgcttgt ctttatacat cagccacacc ttcacatagg ggtctgatgt
     4861 gcccccgatg tccatggctt tgaggtttcg agctttgatg atgttcacga tgatggagtt
     4921 ggcagagggg ttgtagcaga gggacaagag cagctcccct cggctcccac tcccatcgct
     4981 gcatggcttc agatccttcc agaaggtctg catctgggtc aggtccacct tgttcagagg
     5041 gatggacacc tccccaatgg ggtcattgcg gctgaaacgg tcataatcca ggacctggag
     5101 gtagaggacc ctctgcacca ctttctcgta gggaaaacct tcaaatagaa aggtctcatt
     5161 ccagtgcggg tttagattct tccgcttcac cttggtctcc agtttgtgct tcttgtcggg
     5221 tagcaggtag atcttgacaa aggggtcact agtaccgctg aagtccttgg ctggcagctc
     5281 ttgggccttc atgaccttca cggtgagtgt ggactcttgg aagttgtagc caacactgaa
     5341 ctggattcgg cccaggttct ctcggctgca gccctcgtgg gcctcatcct cctcagaacc
     5401 tggggagagc atgagcatct cgctggtaag ggagttgacg aggtccgaga cagaggaacg
     5461 ggtctccgtt ctgcggtcag actcatcgtg tggcgtctgg ccgggcactg gggctgtatt
     5521 cacagccttc cctcctgcag gcaacttgat ggctttcttc tcaccgcgcc cacgccccga
     5581 gtctggcgtg cccaccgtct ccaaggaatt cttgtagcgt ttgcccagtt tgcgctgaca
     5641 ccagtggcac aggccgcaga ggacgatagt gacgctaagg ctgacggtga tgattgcaga
     5701 gaccagcagg acatcgcggg taggtgcccc tgggctggcc gcctccgggt cccggtacat
     5761 ggtggcgggc ccgcggtacc ggatccgcta gccgtacctg cgctctcagg cacgacacga
     5821 ctcctccgct gcccaccgca gactgaggca gcgctgagtc gccggcgccg cagcgcagat
     5881 ggtcgcgccc gtgcccccct atctcgcgcc tcgcgtggtg cggtccggct gggccggcgg
     5941 cggcgcggac gcgaccaagg tggccgggaa ggggagttta cgggggaccg gcgagtgacg
     6001 tcagcgcgcc ttcagtgctg aggcggcggt ggcgcgcgcc gccaggcggg ggcgaaggca
     6061 ctgtccgcgg tgctgaagct ggcagtgcgc acgcgcctcg ccgcatcctg tttcccctcc
     6121 ccctctctga taggggatgc gcaatttggg gaatgggggt tgggtgcttg tccagtgggt
     6181 cggggtcggt cgtcaggtag gcacccccac cccgcctcat cctggtccta aaaccacttg
     6241 cactttaatt aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     6301 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     6361 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     6421 tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc
     6481 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     6541 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     6601 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     6661 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     6721 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     6781 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     6841 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     6901 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     6961 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     7021 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     7081 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     7141 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     7201 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     7261 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     7321 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     7381 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     7441 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     7501 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     7561 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     7621 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     7681 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     7741 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     7801 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     7861 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     7921 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     7981 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
     8041 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     8101 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     8161 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     8221 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     8281 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     8341 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     8401 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     8461 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     8521 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     8581 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     8641 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     8701 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     8761 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     8821 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8881 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8941 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9001 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9061 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9121 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9181 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9241 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9301 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9361 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9421 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9481 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9541 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9601 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9661 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9721 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9781 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9841 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10141 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10741 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10801 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10861 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10921 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10981 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
    11041 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
    11101 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
    11161 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
    11221 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
    11281 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg
    11341 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
    11401 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
    11461 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
    11521 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
    11581 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
    11641 tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt
    11701 atactatgcc ga
//
