LOCUS       sequence-418119-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for AFD neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH64 - [AFDp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1899..1915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1899..1915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1912..1934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2087..2675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2167..2186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2792..2816)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2916..3707,3708..3776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3539..3558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3777..3881)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3980..4002
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3994..4011
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3995..4011
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4635..4663,1..5))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gttatgatat caaaaatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
       61 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
      121 cgctaatgcc tgtgaataga gttctcgtaa atcgacaatg gagttttcaa aaaatacata
      181 acgatgagta atccttgtta tattttccat tagagtaaat tgatataaag aaaacagagt
      241 tatatctgaa gttgagaagc ttaaaagatt tatgactacc gtaagaatat ccggatgagc
      301 tgatacagat ggctcatgaa gtgacgcaac gggtttcatt tcgtacattt ttatcaactc
      361 atattcgatc cacggtaact atcgtgagaa gctgtaaaag gattttcgat tctacctttt
      421 ccacataaaa agagaccaaa aatggggccc tctaagggcg aagaattgtt cacgggagta
      481 gtcccaatct tagtcgagct tgacggaggt gagttttatt atcgattttc gggattttct
      541 acttgtaatt accttttgtt tttaataagt tgcttttttc cagatgttaa tggccacaag
      601 ttttccgtgt ctggagaagg agaaggcgac gctacctacg gtaagctgac cctgaagttc
      661 atttgcacta ctggcaagtt accagtacca tggccgaccc tggtgacaac attttgctac
      721 ggggttcaat gcttctccag atacccaggt aggttttcag tttcaatttt tgttgcaact
      781 acctcaactc tatgttttca gatcatatga aaagacacga cttttttaaa tccgccatgc
      841 cagagggtta cgttcaagaa cgtaccattt tttttaagga cgatggtaac tacaagacac
      901 gtgcggaggt taaatttgaa ggcgatactc tcgtgaaccg tattgagctc aaggtaaatt
      961 tttcaagctt ctaaattcca aaataaacca ttttaatttc ttaattccag ggaatcgatt
     1021 tcaaggaaga tggaaacatt ttgggccaca agcttgaata taattataat agtcataatg
     1081 tctacattat ggccgacaaa caaaaaaacg gtattaaggt taacttcaag attcgccaca
     1141 atatcgaaga tggatctgtc caacttgctg accactacca acaaaatact ccaattggag
     1201 atggaccggt tttgcttcca gataaccact acctttccac tcagtctgcc ctctctaagg
     1261 atccgaatga aaaacgtgac catatggttc ttttggagtt cgttaccgca gcaggaatta
     1321 ctcacggaat ggatgaactg tacaagcccg ggtgatggtc tctgagctat gcaaaatcct
     1381 ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct
     1441 aatttatttg cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg
     1501 catctgagtg aagtgaatgc tatcaaaatg tgattctgat atccaggaaa cggagaatct
     1561 gtgcggcaat gtcttatcat ttgaaacgcg ccgtatcgat gctgacatca tgcggctccc
     1621 gtgttagtcg agaagatggg tacgggcctt tattcatcca acactctacg gcttcttcaa
     1681 gagctagtag ggcaccctgt agttggaaag ggattatttc gtatggcgag cccataccgt
     1741 ctttcttgcg gaagcttaac acgataggaa gttggatagt ttcgaacgat ggttccatac
     1801 ggaacgctgt ctcaaggatg agtctgacgg agtgtaactc gaaggctagg tggaggctca
     1861 gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt catagctgtt tcctgtgtga
     1921 aattgttatc cgctcagagg gcacaatcct attccgcgct atccgacaat ctccaagaca
     1981 ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag
     2041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2701 ggtctgacgc tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg
     2761 gcttcaaatc gtcctcgtga taccaattcg gagcctgctt ttttgtacaa acttgttgat
     2821 aatggcaatt caaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagag
     3061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3181 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3241 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3301 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3361 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3421 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3481 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3541 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3601 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3661 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3721 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3781 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3841 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3901 ccagatacct gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca
     3961 ccacaagcgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa
     4021 tccgcacgca tctggaataa ggaagtgcca ttccgcctga cctaagtgct tttcgagatc
     4081 gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc aagatacgtg
     4141 cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac tgtggaattc
     4201 aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt cttcaaagat
     4261 cgccttctga agtgttttga ctttgaattc ggattctgta ttccgaattc acgaaacaac
     4321 tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc attattctaa
     4381 aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa caatccaaac
     4441 gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca caacaaagcc
     4501 gactactcat atgatgcata aatcttcatt gttgagttta tcttgttgat ttttgaataa
     4561 attatcaact ctttactttt taatgggtta tgaaataaat aaacattgaa aactgataaa
     4621 caacgttcat ctctataact tcgtatagca tacattatac gaa
//
