LOCUS       sequence-418120-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH66 - [BAGp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    760..793
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1417..1434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1417..1433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1426..1448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1547..1651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1652..1720,1721..2512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1870..1889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    2612..2636
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      2753..3341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3242..3261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3494..3516
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3513..3529
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3513..3529
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaaactgaaa acctacctgg gtatctggag aagcattgaa ccccgtagca aaatgttgtc
       61 accagggtcg gccatggtac tggtaacttg ccagtagtgc aaatgaactt cagggtcagc
      121 ttaccgtagg tagcgtcgcc ttctccttct ccagacacgg aaaacttgtg gccattaaca
      181 tctggaaaaa agcaacttat taaaaacaaa aggtaattac aagtagaaaa tcccgaaaat
      241 cgataataaa actcacctcc gtcaagctcg actaagattg ggactactcc cgtgaacaat
      301 tcttcgccct tagagggccc catttttggt ctctttttca tctcatatga ggggcattta
      361 tcccgcaaac agtaacaatt cttatatatt ttttacagga atgtgttgat ggaacgaatt
      421 ccaatatatg tagaagtaaa atgtaaaaaa ttcggagtag ttggattgat gaatctccga
      481 aggaggtgtg aagtcttatg tagccactca cttcttatca tgccggaagg ggccatcgga
      541 agtggataga tggggtttaa tggaaagaag gcaagtgtgt gtcacaacaa gcgtcgaatc
      601 ctacgttttt ctgatgtaca ctgatgacta ggttggcatt agcgtatcca tcgttgtgag
      661 tgatttcagc catttttaag ctctgaaaat ttaaatgaga atagttagtt agtaagatca
      721 tgtttaaact tacagcttgg cgtaatcatt tttgatatca taacttcgta taatgtatgc
      781 tatacgaagt tatagagatg aacgttgttt atcagttttc aatgtttatt tatttcataa
      841 cccattaaaa agtaaagagt tgataattta ttcaaaaatc aacaagataa actcaacaat
      901 gaagatttat gcatcatatg agtagtcggc tttgttgtgc atgacgagtt tgttatcgac
      961 gaaatagaag ctgtcagaac gagtctcgtt tggattgttg atcatgtcgt ccactgaaaa
     1021 agagattagt cttttgaatt gtacttttta gaataatgac tcactgagtt gttgagagag
     1081 ttgagggaac tcatagatat gttcacagtt gtttcgtgaa ttcggaatac agaatccgaa
     1141 ttcaaagtca aaacacttca gaaggcgatc tttgaagaag tgacgttcga tcattcggaa
     1201 atgatggatt gggatgtctc ctactttgaa ttccacagtt gctccgaccg ttttgagttt
     1261 caaaaagttt ggagcaaatc tataacgcac gtatcttgcc gactcctgtg gcgactcatc
     1321 attctcctga tcgttctccg gtttggcgat ctcgaaaagc acttaggtca ggcggaatgg
     1381 cacttcctta ttccagatgc gtgcggatta tgcatgactg gccgtcgttt tacaacgtcg
     1441 tgactgggaa aaccctggcg cttgtggtgg atcacagtta ttggagactt ccttggccgt
     1501 acgatgggtt ttgtttcagg tatctggcac ttttcgggga aatgtgcgcg gaacccctat
     1561 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1621 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1681 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1741 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1801 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1861 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1921 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1981 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2041 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2101 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2161 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2221 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2281 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2341 tgataaatct ggagccggtg agcgtggctc tcgcggtatc attgcagcac tggggccaga
     2401 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2461 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2521 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2581 ctaggtgaag atccttgaat tgccattatc aacaagtttg tacaaaaaag caggctccga
     2641 attggtatca cgaggacgat ttgaagcccc ctacccattt acgctgactt gacgggacgg
     2701 cggctttgtt gaatagagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2761 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2821 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2881 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2941 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3001 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3061 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3361 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcca gcgacatatg
     3421 ccatacgccg aactgaactc cacctaatgt cttggagatt gtcggatagc gcggaatagg
     3481 attgtgccct ctgagcggat aacaatttca cacaggaaac agctatgacc atgacacatg
     3541 catccaccat cgcagactta tcatcactga gcctccacct agccttcgag ttacactccg
     3601 tcagactcat ccttgagaca gcgttccgta tggaaccatc gttcgaaact atccaacttc
     3661 ctatcgtgtt aagcttccgc aagaaagacg gtatgggctc gccatacgaa ataatccctt
     3721 tccaactaca gggtgcccta ctagctcttg aagaagccgt agagtgttgg atgaataaag
     3781 gcccgtaccc atcttctcga ctaacacggg agccgcatga tgtcagcatc gatacggcgc
     3841 gtttcaaatg ataagacatt gccgcacaga ttctccgttt cctggatatc agaatcacat
     3901 tttgatagca ttcacttcac tcagatgcaa acttttcagt gactataaaa agtcataaaa
     3961 taataacatt aaaaaagcaa ataaattagc gagagaattt tttgacaaaa agaaagaaga
     4021 gtgatagaga agaagggaat gcttgaaagg attttgcata gctcagagac catcacccgg
     4081 gcttgtacag ttcatccatt ccgtgagtaa ttcctgctgc ggtaacgaac tccaaaagaa
     4141 ccatatggtc acgtttttca ttcggatcct tagagagggc agactgagtg gaaaggtagt
     4201 ggttatctgg aagcaaaacc ggtccatctc caattggagt attttgttgg tagtggtcag
     4261 caagttggac agatccatct tcgatattgt ggcgaatctt gaagttaacc ttaataccgt
     4321 ttttttgttt gtcggccata atgtagacat tatgactatt ataattatat tcaagcttgt
     4381 ggcccaaaat gtttccatct tccttgaaat cgattccctg gaattaagaa attaaaatgg
     4441 tttattttgg aatttagaag cttgaaaaat ttaccttgag ctcaatacgg ttcacgagag
     4501 tatcgccttc aaatttaacc tccgcacgtg tcttgtagtt accatcgtcc ttaaaaaaaa
     4561 tggtacgttc ttgaacgtaa ccctctggca tggcggattt aaaaaagtcg tgtcttttca
     4621 tatgatctga aaacatagag ttgaggtagt tgcaacaaaa att
//
