LOCUS       sequence-418178-       17238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BGC08 pathway. SGE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 17238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-BGC08-SGE
FEATURES             Location/Qualifiers
     source          1..17238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..36
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        292..635
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             666..1475
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(733..752)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1481..1678
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             1815..1832
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1885..1903)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1899..1946
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1973..1992
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1973..1991
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        11947..12038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12039..12107,12108..12899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      12903..12942
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            13089..13197
                     /note="incP origin of transfer"
     rep_origin      13216..13604
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             15402..17219
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     promoter        complement(17220..17238)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(17221..17238)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gggtgccagg gcgtgcccgt gggctccccg ggcgcgtata gggataacag ggtaatacgt
       61 caaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
      121 ctttgaaaac aactttgaaa aggctgtttt ctgtatttaa ggttttagaa tgcaaggaac
      181 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
      241 aaaagaaatt gcataccttt gttcctcggt tatatgtttg ctcatctgca agacatggag
      301 gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
      361 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
      421 ggccgcacgg cgcgaagcaa aaattacggc acctcgctgc agacctgcga gcagggaaac
      481 gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa
      541 ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga
      601 tacagttctc acatcacatc cgaacataaa caaccaaaaa tttgtaatta agaaggagtg
      661 attacatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg
      721 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      781 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      841 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      901 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      961 tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1021 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1081 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     1141 ttgatgtgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     1201 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1261 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1321 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1381 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1441 tgcagtttca tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct
     1501 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     1561 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgtccag atgcgaagtt
     1621 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     1681 ctagcataac cccgcggggc ctctttcggg gatctcgcgg ggttttttgc tgaaagaagc
     1741 ttcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttatgtt gttgtcgctg
     1801 cggccgcact cgagcaccac caccaccacc actgggatcc ggctgctaac aaagcccgaa
     1861 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     1921 ctaaacgggt cttgaggggt tttttgctga aggccatcat ggccgtttaa actaatacga
     1981 ctcactatag ggagacttaa attttttcat ttttttaacc gcgccactga aattttagca
     2041 tgcgaacaga ggggccctcc ttgaacgatt tttcacaaag aaaaaaagtt ttttatataa
     2101 aagcaatact tgggtcgact tgttatacgc ggatttttaa gtatttaccg tctaaatttt
     2161 taactaacta aaggaatacg ggagtagact atcgaggagt ataaaaatgt taagaggagt
     2221 aggaatcgat atagtagacc tcgaccgtat ccgtgaacag attaaaaaag gtgatctgtt
     2281 tgcgaaacgt gttctgacct ccaaagaatt tgagcagtac cagaagtacc aggaaaaccg
     2341 tcaagtagaa tacctggcgg gtcgtttcag ctgcaaagaa tcgtttagca aggcgatggg
     2401 tgtaggtctg ggcaccatcg ttaagttcaa ggaagttgaa attattgata acgacctggg
     2461 ccagccggaa atgactgcga agtcgtacca cggccgcatc ttcgttagca tcagccatga
     2521 aaagcgttac ttagtaaccc aagtagtact ggaagatatc tagtaatata tatatataac
     2581 tgtctagaaa taaagagtat catctttcaa aatttttttt ttgtctcccg cgccacagag
     2641 gggcgggggc ggtgactgaa attttttttg acaagtcaag tcaggaaaaa aaaatataaa
     2701 agggcgctgc gtaaggagtg ctgccaggtg gttttttaag aaatcaaaag aataattttt
     2761 aagtaactaa cgtatatttt atccaccgat cagaggagcc gaaaaatgaa aaaagaacgc
     2821 aaaaacatat ttctgctgct gagcgcgaag gttatcagca acattggtga taattttttc
     2881 gacttcagca acaacacctg gatcgcaagc ctcggtgcga aaggtcaaaa gattctgacc
     2941 atctaccaga ttacggaatc catcactggt atcgttctga acgtatttgg tggtgttttt
     3001 gcggacaact ataaccgcaa gaagataatc accatcaccg acctgatcgc aggcgtagcc
     3061 tgcctcgttc tgagcatcgt atacccgacc aaatttttcg tctttgccat gatcctggtt
     3121 aacatcatcc tggcaatcct gagcagcttt cgttctccgg catacaaaag cattatccca
     3181 aataccgttc acaaagataa gatcgttaaa gttaatagca acctggaaat ttgcaatcag
     3241 ctcgtaagcg tctctagccc agttatcagc ctgatcgcgt acaaggtttt tggtatctgc
     3301 ggtgcgctgc tgattgacgg cattagcttc ctgatttccg ccctgctgaa caactgtctg
     3361 caggttattc acaaaaaaag cgatgaaaac atccgtcaca ttaaaaacaa aatcatcaaa
     3421 aacatcatcg aaggtatcga atacattacc aagaactcac aagtcgccat cctgctgatt
     3481 gtatccagcg cggttaattt ttttctggcg ggctacaact atagctttcc gttcgttaat
     3541 aaggcattcg gtacgggtaa cagcacctac actatcctcc tggtttcgca gagcctgggc
     3601 ggtatcatcg gcggcatcat tagcccgaaa ctgacgaaaa acgactccat ctcgcatctg
     3661 ctgaatctgc tgacgttctg cggcctgtcc attgccggta tctatctgct attccagtgg
     3721 taccacagcc tgctgaccgt tttcgttttc atgttcctgt tccatatgct gctgacggcc
     3781 tacaacatcg acttctttag catcattcaa acgaaggttg acgacaaata cctgggtcgc
     3841 gtcttcagcg tagtctttac cgttgcaatc atcttcatgc cgatcggtac cgcggttttc
     3901 tcctattaca gcaaagccta cgttgtcgaa aactacatta tcatcggcat cggtattatc
     3961 atcgttagca tcgtagcaca agtactgagc cgtttcaagg gtagcaagta gtaatatata
     4021 actgtctaga aataaatttt ttcaaatttt tttttttttc cttccccgcg ccactgaaat
     4081 ttgggggcgg tggctcaacg gcatttttga ggagaagttt ttacaaaaaa actataaaag
     4141 aacgatctac cgactgtttc gcagagggcc ttttttagct actaccccta ttatttttaa
     4201 gtaactaaat taggattcca atatcggtcc caggagcatt aaaatgaaat tcgatgaaga
     4261 atactataaa gaagtaataa agaaatacaa ctacaacccg ctgctgttta acaagatcga
     4321 tttccaactg ccgggtatct ccaagaacga gatgcaactg atgtggctgt gctcctacca
     4381 tagcctgaaa aaccgcaaag tcaattgtga aaacacgctg ttcacgacgg gcatcggtct
     4441 gagcggtgta ccgcacatgg gtacgatcag ccaaatcatt cgcgcgatta aactccaacg
     4501 tggtggcaac aaagttcaga tcgtactcgg tgacatcgac gcgtatactg gtcgtcacat
     4561 cagctggacg cagacccagc atctgaagga aaagtatcgt aacttcattc tgaaactggg
     4621 tttcaactcc cagaccggca tcctgcgtgc acagtctgac aagtccgttt tcctgaccca
     4681 ggctaaaatt gcgggttact ttaaagatga aatcgcggac gaatccgaag aagacctgca
     4741 cgatctgtac gtaaaggata acaaagtaga gtctaagatg acctttaacc gcaaatacag
     4801 cctgagcctg atgtgcgccg actttatcga accgctgctg atggataact ttaccaacat
     4861 cgtagtcttc ttaggtctgg acgagcacaa atacgtacgt gcgtccaaac aagtcatgga
     4921 cctgatgaaa caagaattca aggttctgga tagcaagaaa atttacgcac tgtattcccc
     4981 gctgatccgt ggttttaacg gcttcccgaa aatgagcaag agctttacgg aaagttctct
     5041 gagcatccag aacaccccgt tcgaaatcga acacaaaatc atgaacaaaa aagaggacga
     5101 aatgccgctg caactgaacg ttaattacca actgatgaaa ggtctgatgg acatcagcga
     5161 ggaaaaagcg aaacaagttc acagcgatta caagaaccaa aatgtaggct ggatcgtaga
     5221 taaagaagag ctgctgaagc gtgttatcga aattcgggat atgtggtacg aatagtaata
     5281 tataactgtc tagaaataaa gagtatcatc taaaaagcac tttttttttt ttacccgcgc
     5341 ccctccttga agggggcggt ggctcaacgg cactgaaatt tacagagggg caagaagtta
     5401 cgaaaaaaaa tcttttttta ttataaaagc aatacttggg tcgacttgtt atacgcggat
     5461 ttttgtaaga acatcacaca aatttttaac taagtaaaat tcttacacgc ggccacgttt
     5521 aggaggcaca aaatgaacga caaatttaac caggtaaatt taatctcaaa acagctgcaa
     5581 caaatcgtca acgacaagaa gtacgacatc ctcgaattaa aagacattct gtactactta
     5641 atgagcagcg acaagaaata ttccgacgcg ttcctgtcgt ccctgctgat gggtttagaa
     5701 ctggtaaaca tgccgagcaa agacattaag acgctgattg acgttattat ccgcattaac
     5761 cattacaacc cgttcagcgt tagcctgtca aaatacccaa acattacttg cgttgcgggc
     5821 agcggcaaaa aatattacaa aactctgaac atcacgaccc tggcgacgat cgttgcgtcc
     5881 ggtctgggtg cggttatcgt taaacctacc agcaagggtg taacgagccg tctgggcagc
     5941 gaagatctgc tggaatatct gaaactgccg atcaccaact cgattaccaa ggctgaagcc
     6001 ttcatcagca aaaactccat cgcgttcttc agcatcgaaa acctgattcc gaaattcgac
     6061 gctatatacg gtggtcgcca actgacgatc tctccgctgt ctaacgcgct gccggggctg
     6121 atgagcccaa tctcttgcca gaacatctat ttcggtctga gcagcaacta tcataacaaa
     6181 gcgctcaacc tgctgcgtag ctatggtatt ggcgggaaca tctcgatttc atcgacgaag
     6241 ctgaatgagg gttatctgga cgagctgacc ttcagcagcg gttccaagct gacctttgaa
     6301 tccagcaata actccacgat catcttagaa ggtatcacct tcgacaaagc agatctgaac
     6361 aacgcccgtt cgaccaataa catcaccaac aacattaaca agatcattag cgagatcaag
     6421 agcacagaag tcaccgatta catgaagatc gtatgtctga acgcagccgg tattctgctg
     6481 acggcgggtg tttacaaaaa cattcgtgta gcatatgaac aagcgaaact ctatgtccag
     6541 aagggaaaac tgctggaccg tattaatgcg ctgcgtggga agcaactgta gtaatatata
     6601 actgtctaga aataaagagt atcatccaaa aaaatttttt tttttcaccg cgccgctcaa
     6661 cggcacagag gggccctcct tgaatagcat gtgaactgaa atttatctca tttttttttt
     6721 ttatttcgcg taatataaaa gcaatacttg ggtcgacttg ttatacgcgg atttttaaga
     6781 aagcatagca atctttttta actaactaat attattattc acaggtattt ttaggagact
     6841 taaaatgtca ttattcgagc aattattaaa cttaaaacaa tcgccgaagg tcgttgttaa
     6901 ggactgcaac aacgattata gcggccgtca actgctggag aaggttggta agatccagaa
     6961 cgtactgctg catctgggcg ttaaaaacgg cgataaaatt ccggttatcg tcgaaccatc
     7021 cattgacgca attgctctgg tttacgccat tggttttctg ggcgcggttt acgttccgat
     7081 tagcccgaaa agcccgaaga agcgtatcaa cttcatcatt aaagacctgt ctgccaagct
     7141 ggttcttacc aatcaaaact acctgctgat agataacaat atcaacatgt tcactatcaa
     7201 agacttactg agcatgacga tcaaggacaa aaccccgacc gtaatgagcc gtcgtcagaa
     7261 cgataccgcc tacatcatct atacgtccgg tagcacgggc aatccgaagg gtgttgaagt
     7321 tagcttcgac aacctggaat cctttatcca cagcttcaac atttgttttc cgagctccca
     7381 aaacagcacc tatctgctga acacggcact gcagtttgac gtatcgatct ccgaactcta
     7441 cggttggatc agccaaggtg cagtactgca gctgctgtcc gcggagcagc tgaaagatct
     7501 taaaaacctg ccgctgtatg ttaagaagta tcacatcacg cacctgtgtg cagcgccgtc
     7561 cacgttacaa gttatggata actcccgtct ggacctgctg gatgaatcca gcctgtcatt
     7621 cctgctgatc gccggtgaag agttcccggt atctctggcg aaacgtctga agaagctggt
     7681 taagagcggt aaggtctata actgctacgg cccgaccgaa gcgacagttt acgcgctgta
     7741 ccacaaagtt accatgaaag acattctgca gggtaaaatc ccgattggtc gtccgttccc
     7801 aaacgcgacc atcaagctgc tgccgcaaaa acgcaagaac tttgaactgc tgatcggtgg
     7861 ctacggcgta tccaaaggtt atctgaacct gaagcgtaag aatagctaca gctttgttga
     7921 actcgatggt gagcgtttct ataaaacggg tgacctggtt aagtacgacc aagacatgaa
     7981 caacatcatc tttgttggcc gtaaggactt tcagttagaa attaacagca ttcgtgttga
     8041 gccgagcgaa atcgagggcg ttattaacca agtcgataaa gttatcgact ctctggtcgt
     8101 aatgaacaag ggcatcctga cgtgcatcta cacgaccttc aagcacagca tcgtagacag
     8161 caacagcatc aaactgaaaa tcagcgaaaa actgccgaag tacatgattc cgaaacgttg
     8221 gtaccgtgtc agcgaatttc cgctgaccat taatggtaag atcgaccgca aaacgctgct
     8281 gaaaacgttc tcgaatccga ttaacaacag cgacacggaa gacaaattaa tctccaaaat
     8341 tgaacagatt tgcgacatca gcaacctgtc cgaaaacgat aacatctttg atctcggcat
     8401 cgatagcctg atgactgttg aaatagaaat tatgctagaa aaacattacc actctaaaat
     8461 cccgacgggt tttctgtatg aacacccgac ggtagaaaaa atcaaacgtt acttcaaaga
     8521 cgaatcgaag ctgaaattca acaacattga gcagatcctg aaaaagatca cggaacgcta
     8581 cgtaatcgac aaagacaagc tgatcattta ctcgaacgat aaaaacgtcg aaaacaagct
     8641 gcagaagcat ctgtacaacc tgagcctgtt taaagaaatc aaaattaacg acatctcgat
     8701 ctttaacaac gaaaacaatt gtgagacggt agttgacggc ggcaagatca acgaggaccg
     8761 taactacgaa tcgagcattt ttcagaaggt ctacaccacc atcaagctgc cgtccttcac
     8821 ggtcgttcgt ttcagcatcc aggatgacct ggttggcagc aagaccaaga cccttattac
     8881 gcgtctgatt tacacgacgg aagcattccg taccaaaatc atcaagacga ccgatggtaa
     8941 ctacatctca accgtctata ccgatctgaa catccgttct aacatagagg atataaccta
     9001 cctgagcaag gaaaaacagg ttaactacat caacaaaaag attggcaagt acgaaaatca
     9061 aatcctgtct aagctgactc gcaattatct gtacaagatc ctgagctttc gcaccagctc
     9121 gcagaacatt gagctggtca ttatttgcca ccacaacatc tgtgacggtg cgagcgaaca
     9181 gctgcttaac aaaaagattg gacaactgct gaacaaccag tccgtcacag atctaaagat
     9241 gaaggactac ctcgaaacca tcaagatgaa cagcgcaatc gaaaacatcc tgaacgatcc
     9301 ggttatcaaa gaaatcggaa agatgaaaaa ccagatcggc aatgagaaac tgacatggtc
     9361 ggaaaaacgc ctggttggtg tagaaaagct gaacaacttt caaaaaatga tgatggttgc
     9421 aaactacgtt actaaggagt acctgaagga gtccggtcac cagtttatga ccttccagat
     9481 gctgtttaac tttcgtaaag tcaataacaa ggacttctct accctgatca acgactgcca
     9541 cgaaaccctg acgttctttc gccgtaagag cgatacggat attcagttcg ttaacaacct
     9601 tttttaccgt ctgaacgact ttcactataa ccgtggtatc agcaccggtt atgcgatcta
     9661 taacaacttt ccggcattta acaacgaaca gctgcaaatc cagaagatcc tggaagaagc
     9721 gccgattaac attgattata tcggcgaagt tgaaaccaag aacctggata gcaaagccaa
     9781 agctattatt aacctgcgta agcaactgaa agacctgaag catcagatcc gttttacggc
     9841 gttttctagc aagaacgaaa tgttcatcta ccgtatttca ggtgaacaga cttggcaata
     9901 ctagtaatat ataactgtct agaaataaag agtatcatcc aaaaatcgcc acgtttaaat
     9961 cgaccgcgcc actgaaattt cctccttgaa acagaggggc gggggcggtg gctcaacggc
    10021 agcttcttag tttttcacgt atccacttta tataaaaggg cgctgcgtaa ggagtgctgc
    10081 caggtggttt tttagaacat cacacaaaga ttttttaagt aagtaatata taaattctat
    10141 cttatccgaa ggaggcgaaa aatggcgata ctgaaaataa acgtctcaga cactattcct
    10201 ctgtcccaag acatcacgaa gcgcctgatt aacgtaaact acgttggcat ctgtggcagc
    10261 gataagcaaa agctggacga cccgccgatc aagtatctgg gccatgaaat cattggcaaa
    10321 gatatggata atggtaaatt cgtcgtcatc aacccgaaca tcacttgcgg taaatgttac
    10381 agctgcttaa acggttatga aaacctgtgc agcaaggaaa aggccatcgg tacgaacgtt
    10441 aacggtggtt tccagggctg gctgcgtgtt ccgtcgacca atctggaata tattgaaaaa
    10501 ccggatatca agtatatctt tacggacccg ctggcagtag ttctgcacgg tatgtctatg
    10561 attaaaatcc tgtcaaacta caaagttatt gtactgggta acggctccat cgcgaaaatc
    10621 ctggtatggc gtctgaacct gctgggcatc aaaccggatg ttatgtgccg cagcggcatt
    10681 tggaacaata aagaactcac gatcagcaac ttatataaac tgaccgaaaa ggtttccctc
    10741 aactattacg acatcgcttt tgaatgtatc ggttttaaac agagcgaaac tattgaaaaa
    10801 agcctgggca tgatccgccc gaagggtgaa ttcatctgct ttggcgtctt ccctgaagac
    10861 tacagcccga ctctgaaagt tcgtcgtctg ttcgaaaaag aaatcacgat cctgggcgtt
    10921 cgctctttca ccaaaaaaga cttcaaatac gccaagaaca tactggacct gcacggtagc
    10981 aaacttatct cccgtctgtg tatttcggtt gtctcctccg aaaacatcaa cgctattatg
    11041 aatgacatcg aggaccgtaa catcgacaaa gttatagttc gtatgaaata gtaatatata
    11101 actgtctaga aataaaggtg caggcatttc aaaatttttt ttttcggcgc cccgcgccgg
    11161 gggcggtgac tgaaatttgc tcaacggctt cttcttaaca ctttttgcag gaaaaaagta
    11221 taaaagccga tagggtgggc gaaggggcgc aggtcctttt tatcaaaaca aataaaacat
    11281 ttttaactaa gtaaatttcg taggcaaatc tcctcccagg agttttaaaa tgatatcaaa
    11341 aaacttaaaa tcaaatataa tacacgagaa caaaggcgcg atcgttatta tgtctgacaa
    11401 tccggaagtc gaaggtcaca tgctggtttt tgcaaaaaaa cactttccga cgctggaact
    11461 ggcggaccaa agcagctggt actacatcgg tgaaattctg aacgagctgg gtaaacgtct
    11521 gcgctcccag ggctatgatg gttataattt actatcagcc aacggagtat cagcaggtca
    11581 atccgttaag catttccact tccacgtcat tccgcgcaag aagaacgacg ggataaacgc
    11641 actaccaaac ttcgaaacca agccgaacaa taccagcgac ttcttcgaga aagttaagtt
    11701 ctagtaatat ataactgtct agaaataaaa actcatttac ttatgtaggt ttcaaaggct
    11761 cctgaggcca cgggcccggg cctgtgaggc cggaccaaaa cgaaaaaagg cccccctttc
    11821 gggaggcctc ttttctggaa tttggtaccg aggggtgcca gggcgtgccc cagggctccc
    11881 cgggcgcgta taatcgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt
    11941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    12001 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg
    12061 tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc
    12121 tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg
    12181 atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga
    12241 gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac
    12301 aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg
    12361 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga
    12421 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg
    12481 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga
    12541 acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc
    12601 tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt
    12661 ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt
    12721 ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg
    12781 gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga
    12841 tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa
    12901 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt gtccttttcc
    12961 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
    13021 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
    13081 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
    13141 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
    13201 gccggccggc gcgccgatct gaagatcagc agttcaacct gttgatagta cgtactaagc
    13261 tctcatgttt cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa
    13321 ctaaaccctc atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt
    13381 cacgtactaa gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc
    13441 ctctaaggtt ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcctttaa
    13501 ggtttaacgg ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct
    13561 ctcaaagcaa ttttgagtga cacaggaaca cttaacggct gacatggggc gcgcccaggt
    13621 cagtcacagt ggagcctagc actcactcag cgtgacggct cagagcagaa ttcacgagcc
    13681 agaaatagta acttttgcct aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca
    13741 catgcttata atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac
    13801 ataaatgaaa taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa
    13861 aagaaactgt tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccggctt
    13921 acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa
    13981 tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa
    14041 caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt
    14101 taagtattgt ttgtgcactt gcctgcaagc cttttgaaaa gcaagcataa aagatctaaa
    14161 cataaaatct gtaaaataac aagatgtaaa gataatgcta aatcatttgg ctttttgatt
    14221 gattgtacag gaaaatatac atcgcagggg gttgactttt accatttcac cgcaatggaa
    14281 ttaaacttgt tgaagagaat gttcacaggc gcatacgcta caatgacccg attcttgcta
    14341 gccgaattcc agtcaggctg ctagcaccag agctacgtga ccgcaggact agctccagct
    14401 gagcgacagc ggcaagaaag ccatccacgc cgaaaacccc gcttcggcgg ggttttgccg
    14461 catcttgccg taactgacaa attaaccgaa ggtttcttcc ggccactggg atgcaatgac
    14521 cttaccaaca acgctacagg attcgttgca cgggatcatt gggtattgcg gattcagcgg
    14581 ctgcaggaaa acttggccag agtcgcggat cagcttctta aaggtgaatt catcaccacc
    14641 caggcgcgca atgcaaaagt ctcccggctc aaccgcctgc tcaggatcaa ccaggatcag
    14701 cataccatcc ggaaatgacg gcttgctgcc cgtcggggcc gtcatgctgt tgccttctac
    14761 ttccagccaa aacgcgctat cgctcgcttt tttggtagtc gatacccaac gctcagcgtc
    14821 acctttggtg aaggtacgca gttccgggct aaacatacct gcttgaacgt ggctgaagac
    14881 cgggtattcg tattcagagc gcagagacgg ttgcatgcta accgcttcat acatttcata
    14941 gatttcacga gctatagaag gagagaactc ctcgacggaa actttcagaa tcttggtcag
    15001 cagtgcggcg ttgtacgcgt tcagcgcatt aataccgtta aacagagcgc caacgccgct
    15061 ctgacccatg cccatcttgt cggctacgct ttcctgagac agacccagct cattcttttt
    15121 cttctcatag attgctttca gacgacgtgc gtcctctagc tgctcttgtg taagcggctt
    15181 tttcttcgtt gacattttga tcctccttta tatggaggta ctaaatcgga acgttaaatc
    15241 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
    15301 gataatggtt gcatcaaatt tgcgcgccac attattattc atacctttgt ggaccgtatt
    15361 acaaagagaa gtgttaaatc aaacaaaaag gaggattaat catggacacg tacgcgggtg
    15421 cttacgaccg tcagtcgcgc gagcgcgaaa attcgagcgc agcaagccca gcgacacagc
    15481 gtagcgccaa cgaagacaag gcggccgacc ttcagcgcga agtcgagcgc gacgggggcc
    15541 ggttcaggtt cgtcgggcat ttcagcgaag cgccgggcac gtcggcgttc gggacggcgg
    15601 agcgcccgga gttcgaacgc atcctgaacg aatgccgcgc cgggcggctc aacatgatca
    15661 ttgtctatga cgtgtcgcgc ttctcgcgcc tgaaggtcat ggacgcgatt ccgattgtct
    15721 cggaattgct cgccctgggc gtgacgattg tttccactca ggaaggcgtc ttccggcagg
    15781 gaaacgtcat ggacctgatt cacctgatta tgcggctcga cgcgtcgcac aaagaatctt
    15841 cgctgaagtc ggcgaagatt ctcgacacga agaaccttca gcgcgaattg ggcgggtacg
    15901 tcggcgggaa ggcgccttac ggcttcgagc ttgtttcgga gacgaaggag atcacgcgca
    15961 acggccgaat ggtcaatgtc gtcatcaaca agcttgcgca ctcgaccact ccccttaccg
    16021 gacccttcga gttcgagccc gacgtaatcc ggtggtggtg gcgtgagatc aagacgcaca
    16081 aacaccttcc cttcaagccg ggcagtcaag ccgccattca cccgggcagc atcacggggc
    16141 tttgtaagcg catggacgct gacgccgtgc cgacccgggg cgagacgatt gggaagaaga
    16201 ccgcttcaag cgcctgggac ccggcaaccg ttatgcgaat ccttcgggac ccgcgtattg
    16261 cgggcttcgc cgctgaggtg atctacaaga agaagccgga cggcacgccg accacgaaga
    16321 ttgagggtta ccgcattcag cgcgacccga tcacgctccg gccggtcgag cttgattgcg
    16381 gaccgatcat cgagcccgct gagtggtatg agcttcaggc gtggttggac ggcagggggc
    16441 gcggcaaggg gctttcccgg gggcaagcca ttctgtccgc catggacaag ctgtactgcg
    16501 agtgtggcgc cgtcatgact tcgaagcgcg gggaagaatc gatcaaggac tcttaccgct
    16561 gccgtcgccg gaaggtggtc gacccgtccg cacctgggca gcacgaaggc acgtgcaacg
    16621 tcagcatggc ggcactcgac aagttcgttg cggaacgcat cttcaacaag atcaggcacg
    16681 ccgaaggcga cgaagagacg ttggcgcttc tgtgggaagc cgcccgacgc ttcggcaagc
    16741 tcactgaggc gcctgagaag agcggcgaac gggcgaacct tgttgcggag cgcgccgacg
    16801 ccctgaacgc ccttgaagag ctgtacgaag accgcgcggc aggcgcgtac gacggacccg
    16861 ttggcaggaa gcacttccgg aagcaacagg cagcgctgac gctccggcag caaggggcgg
    16921 aagagcggct tgccgaactt gaagccgccg aagccccgaa gcttcccctt gaccaatggt
    16981 tccccgaaga cgccgacgct gacccgaccg gccctaagtc gtggtggggg cgcgcgtcag
    17041 tagacgacaa gcgcgtgttc gtcgggctct tcgtagacaa gatcgttgtc acgaagtcga
    17101 ctacgggcag ggggcaggga acgcccatcg agaagcgcgc ttcgatcacg tgggcgaagc
    17161 cgccgaccga cgacgacgaa gacgacgccc aggacggcac ggaagacgta gcggcgtaat
    17221 ctatagtgtc acctaaat
//
