LOCUS       sequence-418211-        2154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_SarJ-MCD2
FEATURES             Location/Qualifiers
     source          1..2154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(182..276)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(297..956)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     871..890
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1118..1136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1140..1183)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1313..1370
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1394..1411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1565..2153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1645..1664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
      181 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
      241 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      301 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      361 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      421 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      481 tttaaatcaa aactggtgaa actcacccag ggattggctg acacgaaaaa catattctca
      541 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
      601 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
      661 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
      721 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
      781 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
      841 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
      901 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
      961 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1021 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt
     1081 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     1141 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttcggtctca gtacagagac
     1201 tgtcgccgga tgtgtatccg acctgacgat ggcccaaaag ggccgaaaca gtcctctaca
     1261 aataattttg tttaacctct taatcatgct aaggatcgtt tcataagaga cctccggcaa
     1321 aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
     1381 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1561 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctaccagcg
     2101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caag
//
