LOCUS       sequence-418215-        2151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_BydvJ-MCD2
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(182..276)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(297..956)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     871..890
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1118..1136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1140..1183)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1310..1367
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1391..1408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1562..2150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1642..1661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
      181 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
      241 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      301 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      361 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      421 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      481 tttaaatcaa aactggtgaa actcacccag ggattggctg acacgaaaaa catattctca
      541 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
      601 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
      661 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
      721 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
      781 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
      841 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
      901 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
      961 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1021 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt
     1081 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     1141 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttcggtctca gtacagaggg
     1201 tgtctcaagg tgcgtacctt gactgatgag tccgaaagga cgaaacaccc ctctacaaat
     1261 aattttgttt aacctcttaa tcatgctaag gatcgtttca taagagacct ccggcaaaaa
     1321 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     1381 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1561 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct accagcggtg
     2101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa g
//
