LOCUS       sequence-418216-        2153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_ElvJ-MCD2
FEATURES             Location/Qualifiers
     source          1..2153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(188..282)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(303..962)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     877..896
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1124..1142
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1146..1189)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1318..1375
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1399..1416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1570..2153,1..5))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1650..1669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
       61 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga
      181 ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      241 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      301 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      361 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      421 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      481 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgacac gaaaaacata
      541 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
      601 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
      661 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
      721 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      781 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      841 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      901 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      961 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1021 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
     1081 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1141 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttcg gtctcagtac
     1201 agagccccat agggtggtgt gtaccacccc tgatgagtcc aaaaggacga aatggggcct
     1261 ctacaaataa ttttgtttaa cctcttaatc atgctaagga tcgtttcata agagacctcc
     1321 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     1381 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1561 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1981 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctac
     2101 cagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gat
//
