LOCUS       sequence-418217-        2183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_CchJ-MCD2
FEATURES             Location/Qualifiers
     source          1..2183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(181..275)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(296..955)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     870..889
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1117..1135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1139..1182)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1341..1398
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1422..1439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1593..2181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1673..1692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
      181 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      241 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      301 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      361 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      421 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      481 ttaaatcaaa actggtgaaa ctcacccagg gattggctga cacgaaaaac atattctcaa
      541 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      601 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      661 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      721 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      781 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      841 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      901 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      961 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1021 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     1081 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     1141 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tcggtctcag tacagagttc
     1201 cagtcgagac ctgaagtggg tttcctgatg aggctgtgga gagagcgaaa gctttactcc
     1261 cgcacaagcc gaaactggaa cctctacaaa taattttgtt taacctctta atcatgctaa
     1321 ggatcgtttc ataagagacc tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
     1381 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
     1441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1561 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     1621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2101 tccggcaaac aaaccaccgc taccagcggt ggtttttttg tttgcaagca gcagattacg
     2161 cgcagaaaaa aaggatctca aga
//
