LOCUS       sequence-418218-        2153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_AraJ-MCD2
FEATURES             Location/Qualifiers
     source          1..2153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(189..283)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(304..963)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     878..897
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1125..1143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1147..1190)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1319..1376
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1400..1417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1571..2153,1..6))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1651..1670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
       61 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
      181 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      241 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      301 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      361 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      421 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      481 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaca cgaaaaacat
      541 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      601 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      661 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      721 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      781 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      841 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      901 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      961 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1021 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
     1081 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     1141 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc ggtctcagta
     1201 cagagtggtc gtgatctgaa actcgatcac ctgatgagct caaggcagag cgaaaccacc
     1261 tctacaaata attttgttta acctcttaat catgctaagg atcgtttcat aagagacctc
     1321 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
     1381 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1441 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1501 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1561 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1741 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1801 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1861 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1921 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1981 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2041 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgcta
     2101 ccagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa gga
//
