LOCUS       sequence-418222-        2131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_MCD2
FEATURES             Location/Qualifiers
     source          1..2131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(187..281)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(302..961)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     876..895
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1123..1141
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1145..1188)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1191..1210
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     RBS             1250..1258
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1295..1352
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1376..1393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1547..2131,1..4))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1627..1646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
       61 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
      181 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      241 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
      301 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      361 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      421 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      481 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat
      541 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      601 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
      661 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
      721 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
      781 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      841 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      901 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      961 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1021 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     1081 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     1141 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     1201 ccgcttctag aatctagaga cgcataggtc tcagtactct taatcatgct aaggaggttt
     1261 tcataagaga cccatgccgt ctcattagct gcagtccggc aaaaaagggc aaggtgtcac
     1321 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctaccag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat c
//
