LOCUS       sequence-418223-        6227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of NAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, 
            Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S
  TITLE     Control of mitophagy initiation and progression by the TBK1 adaptors
            NAP1 and SINTBAD
  JOURNAL   bioRxiv September 25, 202
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            September 25, 202"
COMMENT     Sequence Label: pBMN-HA-NAP1
FEATURES             Location/Qualifiers
     source          1..6227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(94..111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(265..853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(345..364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1017..1808,1809..1877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1640..1659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1878..1982)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2528..2997
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3062..3419
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3484..3900
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3830..3852
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3868..3884
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3910..4284
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     4222..4238
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             4296..4322
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             5722..6191
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
       61 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      961 tttgcggccg gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1981 cgcacatttc cccgaaaagt gccaccagct ttgctcttag gagtttccta atacatccca
     2041 aactcaaata tataaagcat ttgacttgtt ctatgcccta gggggcgggg ggaagctaag
     2101 ccagcttttt ttaacattta aaatgtttaa aatgttaatt ccattttaaa tgcacagatg
     2161 tttttatttc ataagggttt caatgtgcat gaatgctgca atattcctgt taccaaagct
     2221 agtataaata aaaatagata aacgtggaaa ttacttagag tttctgtcat taacgtttcc
     2281 ttcctcagtt gacaacataa atgcgctgct gagaagccag tttgcatctg tcaggatcaa
     2341 tttcccatta tgccagtcat attaattact agtcaattag ttgattttta tttttgacat
     2401 atacatgtga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     2461 aaggcatgga aaaatacata actgagaata gaaaagttca gatcaaggtc aggaacagat
     2521 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     2581 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     2641 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     2701 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     2761 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     2821 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc
     2881 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     2941 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
     3001 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
     3061 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
     3121 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     3181 ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc
     3241 gtttttgtgg cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc
     3301 cccttagagg agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct
     3361 ccgtctgaat ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc
     3421 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc
     3481 ccgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
     3541 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     3601 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     3661 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     3721 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     3781 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
     3841 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
     3901 cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac
     3961 cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac
     4021 ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga
     4081 ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact
     4141 aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc
     4201 ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc tgccgacccc
     4261 gggggtggac catcctctag actgccgcca ccatgtaccc atacgatgtt ccagattacg
     4321 cttccggact cagatctcga gctcaagctt tggatgcact ggtagaagat gatatctgta
     4381 ttctgaatca tgaaaaagcc cataagagag atacagtgac tccagtttca atatattcag
     4441 gagatgaatc tgttgcttcc cattttgctc ttgtcactgc atatgaagac atcaaaaaac
     4501 gacttaagga ttcagagaaa gagaactctt tgttaaagaa gagaataaga tttttggaag
     4561 aaaagctaat agctcgattt gaagaagaaa caagttccgt gggacgagaa caagtaaata
     4621 aggcctatca tgcatatcga gaggtttgca ttgatagaga taatttgaag agcaaactgg
     4681 acaaaatgaa taaagacaac tctgaatctt tgaaagtatt gaatgagcag ctacaatcta
     4741 aagaagtaga actcctccag ctgaggacag aggtggaaac tcagcaggtg atgaggaatt
     4801 taaatccacc ttcatcaaac tgggaggtgg aaaagttgag ctgtgacctg aagatccatg
     4861 gtttggaaca agagctggaa ctgatgagga aagaatgtag cgatctcaaa atagaactac
     4921 agaaagccaa acaaacggat ccatatcagg aagacaatct gaagagcaga gatctccaaa
     4981 aactaagcat ttcaagtgat aatatgcagc atgcatactg ggaactgaag agagaaatgt
     5041 ctaatttaca tctggtgact caagtacaag ctgaactact aagaaaactg aaaacctcaa
     5101 ctgcaatcaa gaaagcctgt gcccctgtag gatgcagtga agaccttgga agagacagca
     5161 caaaactgca cttgatgaat tttactgcaa catacacaag acatccccct ctcttaccaa
     5221 atggcaaagc tctttgtcat accacatctt cccctttacc aggagatgta aaggttttat
     5281 cagagaaagc aatcctccaa tcatggacag acaatgagag atccattcct aatgatggta
     5341 catgctttca ggaacacagt tcttatggca gaaattctct ggaagacaat tcctgggtat
     5401 ttccaagtcc tcctaaatca agtgagacag catttgggga aactaaaact aaaactttgc
     5461 ctttacccaa ccttccacca ctgcattact tggatcaaca taatcagaac tgcctttata
     5521 agaattaggg atccaccgga tctagataac tgatcacgat aaaataaaag attttattta
     5581 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     5641 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     5701 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     5761 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     5821 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     5881 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     5941 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     6001 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     6061 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     6121 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     6181 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt ttttttt
//
