LOCUS       sequence-418224-        2071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 RBS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-R_MCD17
FEATURES             Location/Qualifiers
     source          1..2071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      189..777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     678..697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     931..948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(972..1029)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      1076..1119
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1123..1141)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1303..1962
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1369..1388)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(1983..2071,1..6)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gagaatccaa gcctcgagct gtcagaccaa gtttactcat atatacttta gattgattta
       61 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      241 ccgctggtag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac
      781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata
      961 acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt
     1021 tttgccggag gtctcttatg aaaccctcct ccgcatgatt aagagtactg agaccgaaat
     1081 catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat
     1141 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc actcgagttg
     1201 atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
     1261 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1321 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1381 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1441 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1501 atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat
     1561 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1621 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1681 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtgtca
     1741 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1801 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1861 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1921 gaattacaac agtactgcga tgagtggcag ggcggggcgt aatttgatat cgagctcgct
     1981 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     2041 gctcggttgc cgccgggcgt tttttattgg t
//
